|Positive WB detected in||A549 cells, Jurkat cells, L02 cells, MCF-7 cells, mouse kidney tissue, mouse lung tissue, rat kidney tissue, SH-SY5Y cells|
|Positive IP detected in||mouse lung tissue, A549 cells|
|Positive IHC detected in||human colon cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||PC-12 cells, MCF-7 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10886-1-AP targets Syndecan-3 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Syndecan-3 fusion protein Ag1317|
|Full Name||syndecan 3|
|Calculated molecular weight||38 kDa|
|Observed molecular weight||60-70 kDa|
|GenBank accession number||BC013974|
|Gene ID (NCBI)||9672|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Syndecan-3 is a member o f the Syndecan proteoglycan family. It plays a role in the organization of cell shape by affecting the actin cytoskeleton, possibly by transferring signals from the cell surface in a sugar-dependent mechanism.
|Product Specific Protocols|
|WB protocol for Syndecan-3 antibody 10886-1-AP||Download protocol|
|IHC protocol for Syndecan-3 antibody 10886-1-AP||Download protocol|
|IF protocol for Syndecan-3 antibody 10886-1-AP||Download protocol|
|IP protocol for Syndecan-3 antibody 10886-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Ryan (Verified Customer) (02-28-2019)
Tissue was fixed in PFA with tris-HCl antigen retrieval ph=6. Co-localisation with microglia based on known markers (not shown).