Syndecan-3 Polyclonal antibody

Syndecan-3 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10886-1-AP

Synonyms

KIAA0468, N syndecan, SDC3, SDCN, SYND3, syndecan 3, Syndecan3



Tested Applications

Positive WB detected inA549 cells, Jurkat cells, L02 cells, MCF-7 cells, mouse kidney tissue, mouse lung tissue, rat kidney tissue, SH-SY5Y cells
Positive IP detected inmouse lung tissue, A549 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inPC-12 cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10886-1-AP targets Syndecan-3 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Syndecan-3 fusion protein Ag1317
Full Name syndecan 3
Calculated molecular weight 38 kDa
Observed molecular weight 60-70 kDa
GenBank accession numberBC013974
Gene symbol SDC3
Gene ID (NCBI) 9672
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Syndecan-3 is a member o f the Syndecan proteoglycan family. It plays a role in the organization of cell shape by affecting the actin cytoskeleton, possibly by transferring signals from the cell surface in a sugar-dependent mechanism.

Protocols

Product Specific Protocols
WB protocol for Syndecan-3 antibody 10886-1-APDownload protocol
IHC protocol for Syndecan-3 antibody 10886-1-APDownload protocol
IF protocol for Syndecan-3 antibody 10886-1-APDownload protocol
IP protocol for Syndecan-3 antibody 10886-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

BMC Cancer

Prognostic significance of the expression of GFRα1, GFRα3 and syndecan-3, proteins binding ARTEMIN, in mammary carcinoma.

Authors - Wu Zheng-Sheng ZS
humanWB

J Cell Sci

Electrophoresis of cell membrane heparan sulfate regulates galvanotaxis in glial cells.

Authors - Yu-Ja Huang
humanIHC

Am J Pathol

No haploinsufficiency but loss of heterozygosity for EXT in multiple osteochondromas.

Authors - Reijnders Christianne M A CM
humanIHC

Am J Pathol

Screening for potential targets for therapy in mesenchymal, clear cell, and dedifferentiated chondrosarcoma reveals Bcl-2 family members and TGFβ as potential targets.

Authors - van Oosterwijk Jolieke G JG

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Ryan (Verified Customer) (02-28-2019)

Tissue was fixed in PFA with tris-HCl antigen retrieval ph=6. Co-localisation with microglia based on known markers (not shown).

  • Applications: Immunohistochemistry,Immunofluorescence,
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Zebrafish retina (Microglia) at 3dpf
Syndecan-3 Antibody Immunohistochemistry,Immunofluorescence, validation (1:100 dilution) in Zebrafish retina (Microglia) at 3dpf (Cat no:10886-1-AP)