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SERBP1 Monoclonal antibody

SERBP1 Monoclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, pig, Canine

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1D4H1

Cat no : 67792-1-Ig

Synonyms

CGI 55, CHD3IP, DKFZp564M2423, FLJ90489, HABP4L, PAI RBP1, PAI1 RNA binding protein 1, PAIRBP1, SERBP1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SERBP1 Monoclonal antibody (67792-1-Ig)

    WB analysis using 67792-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67792-1-Ig (SERBP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Western Blot (WB) analysis of MDCK cells using SERBP1 Monoclonal antibody (67792-1-Ig)

    WB analysis of MDCK using 67792-1-Ig

    MDCK cells were subjected to SDS PAGE followed by western blot with 67792-1-Ig (SERBP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HSC-T6 cells using SERBP1 Monoclonal antibody (67792-1-Ig)

    WB analysis of HSC-T6 using 67792-1-Ig

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67792-1-Ig (SERBP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using SERBP1 Monoclonal antibody (67792-1-Ig)

    IP experiment of HeLa using 67792-1-Ig

    IP result of anti-SERBP1 (IP:67792-1-Ig, 5ug; Detection:67792-1-Ig 1:2000) with HeLa cells lysate 1880 ug.

  • Immunoprecipitation (IP) experiment of HeLa cells using SERBP1 Monoclonal antibody (67792-1-Ig)

    IP experiment of HeLa using 67792-1-Ig

    IP result of anti-SERBP1 (IP:67792-1-Ig, 5ug; Detection:67792-1-Ig 1:2000) with HeLa cells lysate 1880 ug.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using SERBP1 Monoclonal antibody (67792-1-Ig)

    IHC staining of human liver cancer using 67792-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67792-1-Ig (SERBP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using SERBP1 Monoclonal antibody (67792-1-Ig)

    IHC staining of human liver cancer using 67792-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67792-1-Ig (SERBP1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using SERBP1 Monoclonal antibody (67792-1-Ig)

    IF Staining of human liver cancer using 67792-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using SERBP1 antibody (67792-1-Ig, Clone: 1D4H1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). DAPI (blue).

Tested Applications

Positive WB detected inLNCaP cells, MDCK cells, HSC-T6 cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman liver cancer tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67792-1-Ig targets SERBP1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with Human, Mouse, Rat, pig, Canine samples.

Tested Reactivity Human, Mouse, Rat, pig, Canine
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SERBP1 fusion protein Ag30202
Full Name SERPINE1 mRNA binding protein 1
Calculated Molecular Weight 45 kDa
Observed Molecular Weight50 kDa
GenBank Accession NumberBC008045
Gene Symbol SERBP1
Gene ID (NCBI) 26135
RRIDAB_2918556
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The plasminogen activator system (PA) plays an important role in invasion and metastasis of solid tumors. The PA Inhibitor type 1 (PAI-1) is the main physiologic regulator of plasminogen activation. PAI-RBP1 (PAI-1 mRNA Binding Protein 1, also named SERBP1), is an RNA-binding protein containing an RG-rich domain and an RGG box domain that might be methylated by protein arginine N-methyltransferases (PRMTs). SERBP1 was identified as an interacting partner of a Tudor domain containing protein TDRD3, and can localize to cytoplasmic stress granules (SGs).

Protocols

Product Specific Protocols
WB protocol for SERBP1 antibody 67792-1-IgDownload protocol
IHC protocol for SERBP1 antibody 67792-1-IgDownload protocol
IF protocol for SERBP1 antibody 67792-1-IgDownload protocol
IP protocol for SERBP1 antibody 67792-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols