SERBP1 Monoclonal antibody, PBS Only (Capture)

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  WB analysis using 67792-1-Ig (same clone as 67792-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67792-1-Ig (SERBP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67792-1-PBS in a different storage buffer formulation.

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  WB analysis of MDCK using 67792-1-Ig (same clone as 67792-1-PBS)

  MDCK cells were subjected to SDS PAGE followed by western blot with 67792-1-Ig (SERBP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67792-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 67792-1-Ig (same clone as 67792-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67792-1-Ig (SERBP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67792-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 67792-1-Ig (same clone as 67792-1-PBS)

  IP result of anti-SERBP1 (IP:67792-1-Ig, 5ug; Detection:67792-1-Ig 1:2000) with HeLa cells lysate 1880 ug. This data was developed using the same antibody clone with 67792-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 67792-1-Ig (same clone as 67792-1-PBS)

  IP result of anti-SERBP1 (IP:67792-1-Ig, 5ug; Detection:67792-1-Ig 1:2000) with HeLa cells lysate 1880 ug. This data was developed using the same antibody clone with 67792-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67792-1-Ig (same clone as 67792-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67792-1-Ig (SERBP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67792-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67792-1-Ig (same clone as 67792-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67792-1-Ig (SERBP1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67792-1-PBS in a different storage buffer formulation.

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  IF Staining of human liver cancer using 67792-1-Ig (same clone as 67792-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using SERBP1 antibody (67792-1-Ig, Clone: 1D4H1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). DAPI (blue). This data was developed using the same antibody clone with 67792-1-PBS in a different storage buffer formulation.

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  Cytometric bead array sample test of MP51733-1

  Cytometric bead array sample test of MP51733-1, SERBP1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67792-1-PBS. Detection antibody: 67792-2-PBS.

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  Cytometric bead array standard curve of MP51733-1

  Cytometric bead array standard curve of MP51733-1, SERBP1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67792-1-PBS. Detection antibody: 67792-2-PBS. Standard:Ag30202. Range: 3.125-400 ng/mL.

SERBP1 Monoclonal Antibody for WB, IHC, IF-P, IP, Cytometric bead array, Indirect ELISA

Cat No. 67792-1-PBS

Clone No.1D4H1

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, canine

Applications

WB, IHC, IF-P, IP, Indirect ELISA

Synonyms

1D4H1, CGI-55, CHD3IP, HABP4L, PAI RBP1

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Product Information

67792-1-PBS targets SERBP1 as part of a matched antibody pair:

MP51733-1: 67792-1-PBS capture and 67792-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat, pig, canine
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag30202

  Product name: Recombinant human SERBP1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 108-408 aa of BC008045

  Sequence: RVGRRPDQQLQGEGKIIDRRPERRPPRERRFEKPLEEKGEGGEFSVDRPIIDRPIRGRGGLGRGRGGRGRGMGRGDGFDSRGKREFDRHSGSDRSSFSHYSGLKHEDKRGGSGSHNWGTVKDELTESPKYIQKQISYNYSDLDQSNVTEETPEGEEHHPVADTENKENEVEEVKEEGPKEMTLDEWKAIQNKDRAKVEFNIRKPNEGADGQWKKGFVLHKSKSEEAHAEDSVMDHHFRKPANDITSQLEINFGDLGRPGRGGRGGRGGRGRGGRPNRGSRTDKSSASAPDVDDPEAFPALA

• Full Name: SERPINE1 mRNA binding protein 1
• Calculated Molecular Weight: 45 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC008045
• Gene Symbol: SERBP1
• Gene ID (NCBI): 26135
• RRID: AB_2918556
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q8NC51
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

The plasminogen activator system (PA) plays an important role in invasion and metastasis of solid tumors. The PA Inhibitor type 1 (PAI-1) is the main physiologic regulator of plasminogen activation. PAI-RBP1 (PAI-1 mRNA Binding Protein 1, also named SERBP1), is an RNA-binding protein containing an RG-rich domain and an RGG box domain that might be methylated by protein arginine N-methyltransferases (PRMTs). SERBP1 was identified as an interacting partner of a Tudor domain containing protein TDRD3, and can localize to cytoplasmic stress granules (SGs).