SKIL Polyclonal antibody
SKIL Polyclonal Antibody for IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IP, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 19218-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | A431 cells, HepG2 cells, human skeletal muscle tissue |
| Positive IP detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 2 publications below |
| CoIP | See 2 publications below |
Product Information
The immunogen of 19218-1-AP is SKIL Fusion Protein expressed in E. coli.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | SKIL fusion protein Ag5282 |
| Full Name | SKI-like oncogene |
| Calculated molecular weight | 77 kDa |
| Observed molecular weight | 77 kDa |
| GenBank accession number | BC059386 |
| Gene symbol | SKIL |
| Gene ID (NCBI) | 6498 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SKIL antibody 19218-1-AP | Download protocol |
| IP protocol for SKIL antibody 19218-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Biochim Biophys Acta De novo GLI3 mutation in esophageal atresia: Reproducing the phenotypic spectrum of Gli3 defects in murine models. | ||
Cell Death Dis SKIL facilitates tumorigenesis and immune escape of NSCLC via upregulating TAZ/autophagy axis. | ||
J Cell Mol Med MDM2 is implicated in high-glucose-induced podocyte mitotic catastrophe via Notch1 signalling. | ||
J Neurosci The Transcriptional Regulator SnoN Promotes the Proliferation of Cerebellar Granule Neuron Precursors in the Postnatal Mouse Brain. | ||
Cell Death Differ PIAS1 and TIF1γ collaborate to promote SnoN SUMOylation and suppression of epithelial-mesenchymal transition. |
