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SKIL Polyclonal antibody

SKIL Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 19218-1-AP

Synonyms

SKI like oncogene, Ski like protein, Ski related oncogene, Ski related protein, SKIL, SNO, SnoA, SnoI, SnoN

Validation Data Gallery

  • WB analysis of A431 using 19218-1-AP

    WB analysis of A431 using 19218-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 19218-1-AP (SKIL antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IP experiment of HepG2 using 19218-1-AP

    IP experiment of HepG2 using 19218-1-AP

    IP Result of anti-SKIL (IP:19218-1-AP, 4ug; Detection:19218-1-AP 1:500) with HepG2 cells lysate 1600ug.

Tested Applications

Positive WB detected inA431 cells, HepG2 cells, human skeletal muscle tissue
Positive IP detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 19218-1-AP is SKIL Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SKIL fusion protein Ag5282
Full Name SKI-like oncogene
Calculated molecular weight 77 kDa
Observed molecular weight 77 kDa
GenBank accession numberBC059386
Gene symbol SKIL
Gene ID (NCBI) 6498
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SKIL antibody 19218-1-APDownload protocol
IP protocol for SKIL antibody 19218-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Biochim Biophys Acta

De novo GLI3 mutation in esophageal atresia: Reproducing the phenotypic spectrum of Gli3 defects in murine models.

Authors - Lin Yang
View Article
humanWB

J Cell Mol Med

MDM2 is implicated in high-glucose-induced podocyte mitotic catastrophe via Notch1 signalling.

Authors - Hui Tang
View Article

J Neurosci

The Transcriptional Regulator SnoN Promotes the Proliferation of Cerebellar Granule Neuron Precursors in the Postnatal Mouse Brain.

Authors - Xiaoying Chen
View Article
humanCoIP

Cell Death Differ

PIAS1 and TIF1γ collaborate to promote SnoN SUMOylation and suppression of epithelial-mesenchymal transition.

Authors - Ayan Chanda
View Article