CoraLite® Plus 488-conjugated SKIL Polyclonal antibody

• Featured Product
• KD/KO Validated

SKIL Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-19218

Synonyms

SKI like oncogene, Ski like protein, Ski related oncogene, Ski related protein, SKIL, SNO, SnoA, SnoI, SnoN

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Validation Data Gallery

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  IF Staining of HepG2 using CL488-19218

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 SKIL antibody (CL488-19218) at dilution of 1:200.

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Tested Applications

• Positive IF detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-19218 targets SKIL in IF applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SKIL fusion protein Ag5282
• Full Name: SKI-like oncogene
• Calculated Molecular Weight: 77 kDa
• Observed Molecular Weight: 77 kDa
• GenBank Accession Number: BC059386
• Gene Symbol: SKIL
• Gene ID (NCBI): 6498
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SKIL, also known as SnoN, is a mediator of the transforming growth factor-β (TGF-β) signaling pathway and exerts pro-oncogenic activity through inhibiting TGF-β/Smad pathway(PMID: 19114989). SKIL is involved in reduction of autophagy and subsequent tumor cell apoptosis in arsenic trioxide-treated promyelocytic leukemia and ovarian carcinoma. In addition, SKIL may regulate cell division or differentiation in response to extracellular signals.