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  • Featured Product
  • KD/KO Validated

SLC25A3 Polyclonal antibody

SLC25A3 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10420-1-AP

Synonyms

OK/SW cl.48, PHC, Phosphate transport protein, PiC, PTP, SLC25A3

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using SLC25A3 Polyclonal antibody (10420-1-AP)

    WB analysis of HeLa using 10420-1-AP

    WB result of SLC25A3 antibody (10420-1-AP; 1:500; incubated at room temperature for 1.5 hours) with sh-Control and sh-SLC25A3 transfected HeLa cells.

  • Western Blot (WB) analysis of various lysates using SLC25A3 Polyclonal antibody (10420-1-AP)

    WB analysis using 10420-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10420-1-AP (SLC25A3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using SLC25A3 Polyclonal antibody (10420-1-AP)

    WB analysis using 10420-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10420-1-AP (SLC25A3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using SLC25A3 Polyclonal antibody (10420-1-AP)

    IHC staining of human lung cancer using 10420-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10420-1-AP (SLC25A3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using SLC25A3 Polyclonal antibody (10420-1-AP)

    IHC staining of human lung cancer using 10420-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10420-1-AP (SLC25A3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHEK-293 cells, HepG2 cells, HeLa cells, MOLT-4 cells, PC-12 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10420-1-AP targets SLC25A3 in WB, IHC, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SLC25A3 fusion protein Ag0667
Full Name solute carrier family 25 (mitochondrial carrier; phosphate carrier), member 3
Calculated Molecular Weight 40 kDa
Observed Molecular Weight 40-43 kDa
GenBank Accession NumberBC003504
Gene Symbol SLC25A3
Gene ID (NCBI) 5250
RRIDAB_2877735
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The mitochondrial phosphate carrier SLC25A3 belongs to the SLC25 family , which is the largest family of solute carriers. SLC25A3 encodes the phosphate carrier PiC that catalyses the import of phosphate into mitochondria where it is needed for ATP synthesis. SLC25A3 has two isoforms SLC25A3-A (a heart-type isoform) and SLC25A3-B (a liver-type isoform) as a result of alternative splicing of exon 3. It has been reported that SLC25A3 mutations are associated with mitochondrial phosphate carrier deficiency, respiratory distress and hypertrophic cardiomyopathy. (PMID: 15984930, 27780865, 20877624)

Protocols

Product Specific Protocols
WB protocol for SLC25A3 antibody 10420-1-APDownload protocol
IHC protocol for SLC25A3 antibody 10420-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols