SMN-Exon7 Monoclonal antibody

SMN-Exon7 Monoclonal Antibody for IF, WB,ELISA

Host / Isotype

Mouse / IgG1









Cat no : 60255-1-Ig


Component of gems 1, Gemin 1, SMN, SMN1, SMN2, SMNC, SMN-Exon7, SMNT, Survival motor neuron protein, T BCD541

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells
Positive IF detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 60255-1-Ig is SMN-Exon7 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SMN-Exon7 fusion protein Ag16615
Full Name survival of motor neuron 1, telomeric
Calculated molecular weight 294 aa, 32 kDa
Observed molecular weight 40 kDa
GenBank accession numberBC062723
Gene symbol SMN1
Gene ID (NCBI) 6606
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Spinal muscular atrophy (SMA) is an autosomal recessive neurodegenerative disease characterized by loss of anterior horn cells in the spinal cord and concomitant symmetrical muscle weakness and atrophy (PMID: 16364894 ). SMA is caused by deletion or mutations of the survival motor neuron (SMN1) gene. SMA patients lack a functional SMN1 gene, but they possess an intact SMN2 gene, which though nearly identical to SMN1, is only partially functional (PMID: 17355180). A large majority of SMN2 transcripts lack exon 7, resulting in production of a truncated, less stable SMN protein (PMID: 10369862). The level of SMN protein correlates with phenotypic severity of SMA. This antibody, 60255-1-Ig, raised against the C-terminal region (275-294aa) encoded by the exon 7.


Product Specific Protocols
WB protocol for SMN-Exon7 antibody 60255-1-IgDownload protocol
IF protocol for SMN-Exon7 antibody 60255-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols