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SMN (Human-Specific) Monoclonal antibody

SMN (Human-Specific) Monoclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

2C6D9

Cat no : 60154-1-Ig

Synonyms

C BCD541, Component of gems 1, Gemin 1, SMN, SMN1, SMN1,SMN, SMN2, SMNC, SMNT, Survival motor neuron protein



Tested Applications

Positive WB detected inA375 cells, Raji cells, HEK-293 cells, HepG2 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman brain tissue, human heart tissue, human kidney tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inJurkat cells
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

60154-1-Ig targets SMN (Human-Specific) in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen SMN (Human-Specific) fusion protein Ag14333
Full Name survival of motor neuron 2, centromeric
Calculated molecular weight 282 aa, 30 kDa
Observed molecular weight 38 kDa
GenBank accession numberBC000908
Gene symbol SMN2
Gene ID (NCBI) 6607
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The survival of motor neurons (SMN) genes are the disease genes of spinal muscular atrophy (SMA), a common motor neuron degenerative disease. The level of SMN protein correlates with phenotypic severity of SMA. SMA patients lack a functional SMN1 gene, but they possess an intact SMN2 gene, which though nearly identical to SMN1, is only partially functional, because a large majority of SMN2 transcripts lack exon 7, resulting in production of a truncated, less stable SMN protein. This antibody 60154-1-Ig is specific to human SMN2. It can't recognize mouse and rat SMN.

Protocols

Product Specific Protocols
WB protocol for SMN (Human-Specific) antibody 60154-1-IgDownload protocol
IHC protocol for SMN (Human-Specific) antibody 60154-1-IgDownload protocol
IF protocol for SMN (Human-Specific) antibody 60154-1-IgDownload protocol
IP protocol for SMN (Human-Specific) antibody 60154-1-IgDownload protocol
FC protocol for SMN (Human-Specific) antibody 60154-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols