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alpha-synuclein Antibody 17 Publications

Rabbit Polyclonal| Catalog number: 10842-1-AP

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Con: 76 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, ELISA
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Images See all

alpha-synuclein Antibody WB mouse brain tissue 10842-1-AP

mouse brain tissue were subjected to SDS PAGE followed by western blot with 10842-1-AP( alpha-synuclein antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours

alpha-synuclein Antibody IHC mouse brain tissue 10842-1-AP

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10842-1-AP( alpha-synuclein antibody at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

alpha-synuclein Antibody IHC mouse brain tissue 10842-1-AP

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10842-1-AP( alpha-synuclein antibody at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

alpha-synuclein Antibody IF SH-SY5Y cells 10842-1-AP

Immunofluorescent analysis of SH-SY5Y cells using 10842-1-AP(alpha-synuclein antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

alpha-synuclein Antibody IF mouse brain tissue 10842-1-AP

Immunofluorescent analysis of ( 4% PFA ) fixed mouse brain tissue using 10842-1-AP(alpha-synuclein antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:
human, mouse, rat, zebra finches
Positive WB detected in:
mouse brain tissue, human brain tissue, SH-SY5Y cells
Positive IHC detected in:
mouse brain tissue, human kidney tissue, human lung tissue, human placenta tissue, human skin tissue, human testis tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
SH-SY5Y cells, mouse brain tissue
Recommended dilution:
WB : 1:500-1:2000
IHC : 1:50-1:500
IF : 1:10-1:100
Sample-dependent, check data in Validation tab

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
alpha-synuclein fusion protein Ag1285
Full name:
synuclein, alpha (non A4 component of amyloid precursor)
Calculated molecular weight:
14 kDa
Observed molecular weight:
15-19 kDa
GenBank accession number:
BC013293
Gene ID (NCBI):
6622
Gene symbol
SNCA
Synonyms
Alpha synuclein, NACP, PARK1, PARK4, SNCA

Background

alpha-synuclein (α-syn) is a 14-19 kDa phosphoprotein that is primarily localize to the presynaptic terminals of mature neurons, where it is involved in synaptic function and plasticity. Α-syn has drawn intense interest ever since the late 1990s, when the first α-synuclein missense mutation was identified as a cause of familial Parkinson’s disease (PD). Aggregated and hyper-phosphorylated forms of α-syn protein are the pathological hallmark of Lewy body disease, which includes Parkinson's disease (PD), diffuse Lewy body disease (DLBD), and Lewy body variant of Alzheimer's disease (LBV). This antibody can recognize all the isoforms of α-syn.


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