|Positive WB detected in||mouse brain tissue, human brain tissue|
|Positive IHC detected in||mouse brain tissue, human kidney tissue, human placenta tissue, human testis tissue, human skin tissue, human lung tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||SH-SY5Y cells, mouse brain tissue|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
10842-1-AP targets Alpha Synuclein in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, yeast, zebra finches|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Alpha Synuclein fusion protein Ag1285|
|Full Name||synuclein, alpha (non A4 component of amyloid precursor)|
|Calculated molecular weight||14 kDa|
|Observed molecular weight||15-19 kDa|
|GenBank accession number||BC013293|
|Gene ID (NCBI)||6622|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.1% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Alpha Synuclein (α-syn) is a 14-19 kDa phosphoprotein that is primarily localize to the presynaptic terminals of mature neurons, where it is involved in synaptic function and plasticity. Α-syn has drawn intense interest ever since the late 1990s, when the first α-synuclein missense mutation was identified as a cause of familial Parkinson's disease (PD). Aggregated and hyper-phosphorylated forms of α-syn protein are the pathological hallmark of Lewy body disease, which includes Parkinson's disease (PD), diffuse Lewy body disease (DLBD), and Lewy body variant of Alzheimer's disease (LBV). This antibody can recognize all the isoforms of α-syn.
Behav Brain Res
Social context-dependent singing alters molecular markers of dopaminergic and glutamatergic signaling in finch basal ganglia Area X.
Alpha-synuclein overexpression induced mitochondrial damage by the generation of endogenous neurotoxins in PC12 cells.
α-Synuclein binds to cytoplasmic vesicles in U251 glioblastoma cells.
Inactive USP14 and inactive UCHL5 cause accumulation of distinct ubiquitinated proteins in mammalian cells.
Myocardial cathepsin D is downregulated in sudden cardiac death.
The effect of copy number on α-synuclein's toxicity and its protective role in Bax-induced apoptosis, in yeast.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Sheela (Verified Customer) (07-16-2019)
This antibody works as expected!