Alpha Synuclein Polyclonal antibody

Alpha Synuclein Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10842-1-AP

Synonyms

Alpha synuclein, NACP, PARK1, PARK4, SNCA



Tested Applications

Positive WB detected inmouse brain tissue, human brain tissue
Positive IHC detected inmouse brain tissue, human kidney tissue, human placenta tissue, human testis tissue, human skin tissue, human lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inSH-SY5Y cells, mouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

10842-1-AP targets Alpha Synuclein in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat, yeast, zebra finches
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Alpha Synuclein fusion protein Ag1285
Full Name synuclein, alpha (non A4 component of amyloid precursor)
Calculated molecular weight 14 kDa
Observed molecular weight 15-19 kDa
GenBank accession numberBC013293
Gene symbol SNCA
Gene ID (NCBI) 6622
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Alpha Synuclein (α-syn) is a 14-19 kDa phosphoprotein that is primarily localize to the presynaptic terminals of mature neurons, where it is involved in synaptic function and plasticity. Α-syn has drawn intense interest ever since the late 1990s, when the first α-synuclein missense mutation was identified as a cause of familial Parkinson's disease (PD). Aggregated and hyper-phosphorylated forms of α-syn protein are the pathological hallmark of Lewy body disease, which includes Parkinson's disease (PD), diffuse Lewy body disease (DLBD), and Lewy body variant of Alzheimer's disease (LBV). This antibody can recognize all the isoforms of α-syn.

Protocols

Product Specific Protocols
WB protocol for Alpha Synuclein antibody 10842-1-APDownload protocol
IHC protocol for Alpha Synuclein antibody 10842-1-APDownload protocol
IF protocol for Alpha Synuclein antibody 10842-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
zebra finchesWB

Behav Brain Res

Social context-dependent singing alters molecular markers of dopaminergic and glutamatergic signaling in finch basal ganglia Area X.

Authors - Lisa Y So
ratWB

Neurosci Lett

Alpha-synuclein overexpression induced mitochondrial damage by the generation of endogenous neurotoxins in PC12 cells.

Authors - Zhang Yanyan Y
humanWB

Neurosci Lett

α-Synuclein binds to cytoplasmic vesicles in U251 glioblastoma cells.

Authors - Jinyan Duan
humanWB

PLoS One

Inactive USP14 and inactive UCHL5 cause accumulation of distinct ubiquitinated proteins in mammalian cells.

Authors - Jayashree Chadchankar
humanWB

PLoS One

Myocardial cathepsin D is downregulated in sudden cardiac death.

Authors - Yu Kakimoto
yeastWB

Biosci Rep

The effect of copy number on α-synuclein's toxicity and its protective role in Bax-induced apoptosis, in yeast.

Authors - Damilare D Akintade

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Sheela (Verified Customer) (07-16-2019)

This antibody works as expected!

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Mouse brain RIPA lysate
Alpha Synuclein Antibody Western Blot, validation (1:1000 dilution) in Mouse brain RIPA lysate (Cat no:10842-1-AP)