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SPR Polyclonal antibody

SPR Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 16822-1-AP

Synonyms

SDR38C1, Sepiapterin reductase, SPR

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SPR Polyclonal antibody (16822-1-AP)

    WB analysis using 16822-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16822-1-AP (SPR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat liver tissue using SPR Polyclonal antibody (16822-1-AP)

    WB analysis of rat liver using 16822-1-AP

    rat liver tissue were subjected to SDS PAGE followed by western blot with 16822-1-AP (SPR Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat heart tissue using SPR Polyclonal antibody (16822-1-AP)

    WB analysis of rat heart using 16822-1-AP

    rat heart tissue were subjected to SDS PAGE followed by western blot with 16822-1-AP (SPR Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using SPR Polyclonal antibody (16822-1-AP)

    WB analysis of human liver using 16822-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 16822-1-AP (SPR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using SPR Polyclonal antibody (16822-1-AP)

    WB analysis of HepG2 using 16822-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 16822-1-AP (SPR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using SPR Polyclonal antibody (16822-1-AP)

    WB analysis of K-562 using 16822-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 16822-1-AP (SPR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse liver tissue using SPR Polyclonal antibody (16822-1-AP)

    IP experiment of mouse liver using 16822-1-AP

    IP result of anti-SPR (IP:16822-1-AP, 4ug; Detection:16822-1-AP 1:500) with mouse liver tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using SPR Polyclonal antibody (16822-1-AP)

    IHC staining of human liver cancer using 16822-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16822-1-AP (SPR Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using SPR Polyclonal antibody (16822-1-AP)

    IHC staining of human liver cancer using 16822-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16822-1-AP (SPR Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SPR Polyclonal antibody (16822-1-AP)

    IF Staining of HeLa using 16822-1-AP

    Immunofluorescent analysis of Hela cells, using SPR antibody 16822-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inHepG2 cells, rat heart tissue, rat liver tissue, human liver tissue, K-562 cells
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

16822-1-AP targets SPR in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SPR fusion protein Ag10395
Full Name sepiapterin reductase (7,8-dihydrobiopterin:NADP+ oxidoreductase)
Calculated Molecular Weight 261 aa, 28 kDa
Observed Molecular Weight 28 kDa
GenBank Accession NumberBC017310
Gene Symbol SPR
Gene ID (NCBI) 6697
RRIDAB_2286632
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for SPR antibody 16822-1-APDownload protocol
IHC protocol for SPR antibody 16822-1-APDownload protocol
IF protocol for SPR antibody 16822-1-APDownload protocol
IP protocol for SPR antibody 16822-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Chem Biol

Metabolic determinants of cancer cell sensitivity to canonical ferroptosis inducers.

Authors - Mariluz Soula
View Article
mouseWB

Acta Physiol (Oxf)

Tetrahydrobiopterin synthesis and metabolism is impaired in dystrophin-deficient mdx mice and humans.

Authors - Angus Lindsay
View Article
humanWB

Phytomedicine

Cimifugin suppresses type 2 airway inflammation by binding to SPR and regulating its protein expression in a non-enzymatic manner

Authors - Xiaoqun Gu
View Article