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  • KD/KO Validated

SREBF1 Polyclonal antibody

SREBF1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (4)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14088-1-AP

Synonyms

bHLHd1, SREBF1, SREBP 1, SREBP1



Tested Applications

Positive WB detected inMCF-7 cells, L02 cells, HeLa cells
Positive IP detected inL02 cells
Positive IHC detected inhuman kidney tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

14088-1-AP targets SREBF1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity bovine, chicken, cow, goat, human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SREBF1 fusion protein Ag5219
Full Name sterol regulatory element binding transcription factor 1
Calculated molecular weight 1177 aa, 125 kDa
Observed molecular weight 125 kDa
GenBank accession numberBC063281
Gene symbol SREBF1
Gene ID (NCBI) 6720
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SREBF1, also named as BHLHD1 and SREBP1, contains one basic helix-loop-helix (bHLH) domain and belongs to the SREBP family. It is a transcriptional activator required for lipid homeostasis. The SREBPs are synthesized as precursors anchored to endoplasmic reticulum (ER) membranes and complexed with SCAP. When the cellular cholesterol level is low, SREBP-SCAP complexes move to the Golgi apparatus, where SREBPs undergo a two-step proteolytic processing, leading to the release of the mature form, an N-terminal fragment, i.e, basic helix-loop-helix leucine zipper transcription factor. These factors enter the nucleus where they bind to sterol regulatory elements (SRE) in the promoter regions of a number of genes whose products mediate the synthesis of cholesterol and fatty acids. [PMID: 21698267]. This antibody can recognize the 125kd precursor form and the 68kd mature form of human SREBF1.

Protocols

Product Specific Protocols
WB protocol for SREBF1 antibody 14088-1-APDownload protocol
IHC protocol for SREBF1 antibody 14088-1-APDownload protocol
IF protocol for SREBF1 antibody 14088-1-APDownload protocol
IP protocol for SREBF1 antibody 14088-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

J Ethnopharmacol

Chronic treatment with the modified Longdan Xiegan Tang attenuates olanzapine-induced fatty liver in rats by regulating hepatic de novo lipogenesis and fatty acid beta-oxidation-associated gene expression mediated by SREBP-1c, PPAR-alpha and AMPK-alpha.

Authors - Liying Ren
cowWB

J Cell Physiol

NUCKS1 is a novel regulator of milk synthesis in and proliferation of mammary epithelial cells via the mTOR signaling pathway.

Authors - Xiaohan Yuan
cowWB

Cell Biochem Funct

Cyclase-associated protein 1 is a key negative regulator of milk synthesis and proliferation of bovine mammary epithelial cells.

Authors - Xiaohan Yuan
bovineWB

Cell Biochem Funct

U2AF65 enhances milk synthesis and growth of bovine mammary epithelial cells by positively regulating the mTOR-SREBP-1c signalling pathway.

Authors - Yanbo Yu
humanWB

Biochem Biophys Res Commun

Inhibition of fatty acid synthesis arrests colorectal neoplasm growth and metastasis: Anti-cancer therapeutical effects of natural cyclopeptide RA-XII.

Authors - Yurong Wang
  • KD Validated
humanWB

J Cell Physiol

Morin reduces inflammatory responses and alleviates lipid accumulation in hepatocytes.

Authors - Lin Wu