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STK31 Polyclonal antibody

STK31 Polyclonal Antibody for IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP,ELISA

Conjugate

Unconjugated

Cat no : 14140-1-AP

Synonyms

serine/threonine kinase 31, SGK396, STK31, Sugen kinase 396, TDRD8

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using STK31 Polyclonal antibody (14140-1-AP)

    WB analysis of mouse testis using 14140-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 14140-1-AP (STK31 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat testis tissue using STK31 Polyclonal antibody (14140-1-AP)

    WB analysis of rat testis using 14140-1-AP

    rat testis tissue were subjected to SDS PAGE followed by western blot with 14140-1-AP (STK31 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using STK31 Polyclonal antibody (14140-1-AP)

    WB analysis of mouse testis using 14140-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 14140-1-AP (STK31 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using STK31 Polyclonal antibody (14140-1-AP)

    WB analysis of mouse testis using 14140-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 14140-1-AP (STK31 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse testis tissue using STK31 Polyclonal antibody (14140-1-AP)

    IP experiment of mouse testis using 14140-1-AP

    IP result of anti-STK31 (IP:14140-1-AP, 4ug; Detection:14140-1-AP 1:1000) with mouse testis tissue lysate 2400ug.

Tested Applications

Positive WB detected inmouse testis tissue, rat testis tissue
Positive IP detected inmouse testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14140-1-AP targets STK31 in WB, IP,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen STK31 fusion protein Ag5221
Full Name serine/threonine kinase 31
Calculated Molecular Weight 1019 aa, 116 kDa
Observed Molecular Weight 115 kDa
GenBank Accession NumberBC059374
Gene Symbol STK31
Gene ID (NCBI) 56164
RRIDAB_2878021
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for STK31 antibody 14140-1-APDownload protocol
IP protocol for STK31 antibody 14140-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols