STK4/MST1 Polyclonal antibody
STK4/MST1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 22245-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | Jurkat cells, HeLa cells, rat liver tissue, Ramos cells, C2C12 cells, mouse spleen tissue, rat spleen tissue |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human prostate cancer tissue, human prostate hyperplasia tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:50-1:200 |
| Immunofluorescence (IF) | IF : 1:20-1:200 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 8 publications below |
| IHC | See 5 publications below |
| IF | See 2 publications below |
Product Information
The immunogen of 22245-1-AP is STK4/MST1 Fusion Protein expressed in E. coli.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, sheep |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | STK4/MST1 fusion protein Ag17738 |
| Full Name | serine/threonine kinase 4 |
| Calculated molecular weight | 487 aa, 56 kDa |
| Observed molecular weight | 56 kDa |
| GenBank accession number | BC093768 |
| Gene symbol | STK4 |
| Gene ID (NCBI) | 6789 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for STK4/MST1 antibody 22245-1-AP | Download protocol |
| IHC protocol for STK4/MST1 antibody 22245-1-AP | Download protocol |
| IF protocol for STK4/MST1 antibody 22245-1-AP | Download protocol |
| IP protocol for STK4/MST1 antibody 22245-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Theriogenology Expression of Hippo signaling pathway components in Hu sheep male reproductive tract and spermatozoa. | ||
Cell Death Dis Alginate oligosaccharide attenuates α2,6-sialylation modification to inhibit prostate cancer cell growth via the Hippo/YAP pathway. | ||
Front Oncol FAM83A Promotes Lung Cancer Progression by Regulating the Wnt and Hippo Signaling Pathways and Indicates Poor Prognosis. | ||
Tissue Cell Energy restriction affect liver development in Hu sheep ram lambs through Hippo signaling pathway. | ||
Neuroscience Differential Levels of Hippo Signaling in Selected Brain and Peripheral Tissues in Streptozotocin-Induced Cognitive Dysfunction in Mice. | ||
Cell Death Dis REGγ deficiency suppresses tumor progression via stabilizing CK1ε in renal cell carcinoma. |
