STK4/MST1 Polyclonal antibody

STK4/MST1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 22245-1-AP

Synonyms

KRS2, MST 1, MST1, serine/threonine kinase 4, STE20 like kinase MST1, STK4, YSK3



Tested Applications

Positive WB detected inJurkat cells, HeLa cells, rat liver tissue, Ramos cells, C2C12 cells, mouse spleen tissue, rat spleen tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman prostate cancer tissue, human prostate hyperplasia tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 22245-1-AP is STK4/MST1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, sheep
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen STK4/MST1 fusion protein Ag17738
Full Name serine/threonine kinase 4
Calculated molecular weight 487 aa, 56 kDa
Observed molecular weight 56 kDa
GenBank accession numberBC093768
Gene symbol STK4
Gene ID (NCBI) 6789
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for STK4/MST1 antibody 22245-1-APDownload protocol
IHC protocol for STK4/MST1 antibody 22245-1-APDownload protocol
IF protocol for STK4/MST1 antibody 22245-1-APDownload protocol
IP protocol for STK4/MST1 antibody 22245-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
sheepWB,IHC,IF

Theriogenology

Expression of Hippo signaling pathway components in Hu sheep male reproductive tract and spermatozoa.

Authors - Guo-Min Zhang
humanIHC

Cell Death Dis

Alginate oligosaccharide attenuates α2,6-sialylation modification to inhibit prostate cancer cell growth via the Hippo/YAP pathway.

Authors - Yang Han
humanWB

Front Oncol

FAM83A Promotes Lung Cancer Progression by Regulating the Wnt and Hippo Signaling Pathways and Indicates Poor Prognosis.

Authors - Yi-Wen Zheng
sheepWB,IHC

Tissue Cell

Energy restriction affect liver development in Hu sheep ram lambs through Hippo signaling pathway.

Authors - Ting-Ting Zhang
mouseWB

Neuroscience

Differential Levels of Hippo Signaling in Selected Brain and Peripheral Tissues in Streptozotocin-Induced Cognitive Dysfunction in Mice.

Authors - Fan Yu
humanWB

Cell Death Dis

REGγ deficiency suppresses tumor progression via stabilizing CK1ε in renal cell carcinoma.

Authors - Shaojun Chen