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- KD/KO Validated
STK4/MST1 Polyclonal antibody
STK4/MST1 Polyclonal Antibody for IF, IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IP, IHC, IF,ELISA
Conjugate
Unconjugated
Cat no : 22245-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | Jurkat cells, HeLa cells, HepG2 cells, rat liver tissue, Ramos cells, C2C12 cells, mouse spleen tissue, rat spleen tissue |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human prostate cancer tissue, human prostate hyperplasia tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
Immunohistochemistry (IHC) | IHC : 1:50-1:200 |
Immunofluorescence (IF) | IF : 1:20-1:200 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 1 publications below |
WB | See 9 publications below |
IHC | See 6 publications below |
IF | See 2 publications below |
Product Information
The immunogen of 22245-1-AP is STK4/MST1 Fusion Protein expressed in E. coli.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, sheep |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | STK4/MST1 fusion protein Ag17738 |
Full Name | serine/threonine kinase 4 |
Calculated molecular weight | 487 aa, 56 kDa |
Observed molecular weight | 56 kDa |
GenBank accession number | BC093768 |
Gene symbol | STK4 |
Gene ID (NCBI) | 6789 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
Product Specific Protocols | |
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WB protocol for STK4/MST1 antibody 22245-1-AP | Download protocol |
IHC protocol for STK4/MST1 antibody 22245-1-AP | Download protocol |
IF protocol for STK4/MST1 antibody 22245-1-AP | Download protocol |
IP protocol for STK4/MST1 antibody 22245-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Theriogenology Expression of Hippo signaling pathway components in Hu sheep male reproductive tract and spermatozoa. | ||
Cell Death Dis Alginate oligosaccharide attenuates α2,6-sialylation modification to inhibit prostate cancer cell growth via the Hippo/YAP pathway. | ||
Front Oncol FAM83A Promotes Lung Cancer Progression by Regulating the Wnt and Hippo Signaling Pathways and Indicates Poor Prognosis. | ||
Tissue Cell Energy restriction affect liver development in Hu sheep ram lambs through Hippo signaling pathway. | ||
Neuroscience Differential Levels of Hippo Signaling in Selected Brain and Peripheral Tissues in Streptozotocin-Induced Cognitive Dysfunction in Mice. | ||
Cell Death Dis REGγ deficiency suppresses tumor progression via stabilizing CK1ε in renal cell carcinoma. |