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STK4/MST1 Antibody 5 Publications

Rabbit Polyclonal| Catalog number: 22245-1-AP

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Con: 84 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, ELISA
Application not tested? Please see our Guarantee


Images See all

STK4/MST1 Antibody WB HeLa cells 22245-1-AP

HeLa cells were subjected to SDS PAGE followed by western blot with 22245-1-AP(STK4/MST1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

STK4/MST1 Antibody IHC human prostate cancer tissue 22245-1-AP

Immunohistochemical of paraffin-embedded human prostate cancer using 22245-1-AP(STK4/MST1 antibody) at dilution of 1:50 (under 10x lens)

STK4/MST1 Antibody IHC human prostate cancer tissue 22245-1-AP

Immunohistochemical of paraffin-embedded human prostate cancer using 22245-1-AP(STK4/MST1 antibody) at dilution of 1:50 (under 40x lens)

STK4/MST1 Antibody IF HepG2 cells 22245-1-AP

Immunofluorescent analysis of HepG2 cells using 22245-1-AP(STK4/MST1 antibody) at dilution of 1:50 and and Rhodamine-labeled goat anti-rabbit IgG (red).

STK4/MST1 Antibody IP HeLa cells 22245-1-AP

IP Result of anti-STK4/MST1 (IP:22245-1-AP, 4ug; Detection:22245-1-AP 1:1000) with HeLa cells lysate 1200ug.

Species specificity:
human, mouse, rat, sheep
Positive WB detected in:
HeLa cells, rat liver tissue
Positive IP detected in:
HeLa cells
Positive IHC detected in:
human prostate cancer tissue, human prostate hyperplasia tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HepG2 cells
Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:20-1:200
IF : 1:20-1:200
Sample-dependent, check data in Validation tab

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
STK4/MST1 fusion protein Ag17738
Full name:
serine/threonine kinase 4
Calculated molecular weight:
487aa,56 kDa
Observed molecular weight:
56 kDa
GenBank accession number:
BC093768
Gene ID (NCBI):
6789
Gene symbol
STK4
Synonyms
KRS2, MST 1, MST1, serine/threonine kinase 4, STE20 like kinase MST1, STK4, YSK3

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