transgelin/SM22 Monoclonal antibody

transgelin/SM22 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, pig, mouse

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

2A10C2

Cat no : 60213-1-Ig

Synonyms

SM22, SM22 alpha, SM22a, SM22-a, SM22alpha, Smooth muscle protein 22 alpha, TAGLN, TAGLN1, transgelin, transgelin/SM22 alpha



Tested Applications

Positive WB detected inhuman colon tissue, human stomach tissue, pig stomach tissue, rat colon tissue, rat stomach tissue
Positive IHC detected inhuman colon tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse heart tissue
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:400
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 60213-1-Ig is transgelin/SM22 Fusion Protein expressed in E. coli.

Tested Reactivity human, rat, pig, mouse
Cited Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen transgelin/SM22 fusion protein Ag0764
Full Name transgelin
Calculated molecular weight 22 kDa
Observed molecular weight 18+22 kDa
GenBank accession numberBC004927
Gene symbol TAGLN
Gene ID (NCBI) 6876
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The transgelin family is a group of proteins that belong to 22 kDa actin-related corpnin superfamily. Of all three isoforms, transgelin 1 is the best characterized. Transgelin 1, also known as SM22 alpha, is a specific marker for differentiated smooth muscle cells. Transgelin 2, also known as SM22 beta, is expressed by both smooth muscle and non-smooth muscle cells in a temporally and spatially regulated pattern. Trangenlin 3, also known as NP25, is only found in highly differentiated neuronal cells.

Protocols

Product Specific Protocols
WB protocol for transgelin/SM22 antibody 60213-1-IgDownload protocol
IHC protocol for transgelin/SM22 antibody 60213-1-IgDownload protocol
IF protocol for transgelin/SM22 antibody 60213-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanFC

Cells

Characterization of Carotid Smooth Muscle Cells during Phenotypic Transition.

Authors - Haize Goikuria
humanWB

Oncotarget

Specific role of RhoC in tumor invasion and metastasis.

Authors - Sarah Lang