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TDP-43 (C-terminal) Antibody 77 Publications

Rabbit Polyclonal| Catalog number: 12892-1-AP

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Con: 61 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, chip, ELISA
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Images See all

TDP-43 (C-terminal) Antibody WB A549 cells 12892-1-AP

A549 cells (shcontrol and shRNA of TDP43) were subjected to SDS PAGE followed by western blot with 12892-1-AP (TDP43 antibody) at dilution of 1:1000.

TDP-43 (C-terminal) Antibody IHC human gliomas tissue 12892-1-AP

Immunohistochemistry of paraffin-embedded human gliomas tissue slide using 12892-1-AP( TDP-43 (C-terminal) antibody at dilution of 1:1000 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

TDP-43 (C-terminal) Antibody IF HeLa cells 12892-1-AP

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HeLa cells using 12892-1-AP(TDP-43 (C-terminal) antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

TDP-43 (C-terminal) Antibody IHC human gliomas tissue 12892-1-AP

Immunohistochemistry of paraffin-embedded human gliomas tissue slide using 12892-1-AP( TDP-43 (C-terminal) antibody at dilution of 1:1000 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

TDP-43 (C-terminal) Antibody IP HeLa cells 12892-1-AP

IP Result of anti-TARDBP (IP:12892-1-AP, 3ug; Detection:12892-1-AP 1:1000) with HeLa cells lysate 3000ug.

Species specificity:
human, mouse, rat, monkey, zebrafish
Positive WB detected in:
A549 cells, A2780 cells, C6 cells, HeLa cells, human heart tissue, human placenta tissue, K-562 cells, mouse heart tissue, mouse pancreas tissue
Positive IP detected in:
HeLa cells, mouse brain tissue
Positive IHC detected in:
human gliomas tissue, human lung cancer tissue, mouse brain tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HeLa cells
Recommended dilution:
WB : 1:500-1:1000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:100-1:2000
IF : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
fusion protein
Full name:
TAR DNA binding protein
Calculated molecular weight:
43 kDa
Observed molecular weight:
43-45 kDa,35 kDa
GenBank accession number:
BC001487
Gene ID (NCBI):
23435
Gene symbol
TARDBP
Synonyms
ALS10, TAR DNA binding protein, TAR DNA binding protein 43, TARDBP, TDP 43, TDP43

Background

Transactivation response (TAR), DNA-binding protein of 43 kDa (also known as TARDBP or TDP-43), was first isolated as a transcriptional inactivator binding to the TAR DNA element of the HIV-1 virus. Neumann et al. (2006) found that a hyperphosphorylated, ubiquitinated, and cleaved form of TARDBP, known as pathologic TDP-43, is the major component of the tau-negative and ubiquitin-positive inclusions that characterize amyotrophic lateral sclerosis (ALS) and the most common pathological subtype of frontotemporal lobar degeneration (FTLD-U). 12892-1-AP is a rabbit polyclonal antibody raised against the C-terminal amino acids of human TDP-43. This antibody recognizes the cleavage product of 20-30 kDa in addition to the native and phosphorylated forms of TDP-43. Immunohistochemical analyses of TDP-43 using this antibody detect both normal diffuse nuclear staining and insoluble inclusions in pathologic tissues. Various forms of TDP-43 exist, including 18-35 kDa of cleaved C-terminal fragments, 45-50 kDa phosphoprotein, 55 kDa glycosylated form, 75 kDa hyperphosphorylated form, and 90-300 kDa cross-linked form. (17023659,19823856,21666678,22193176)

Recently TDP-43 has been reported to be overexpressed in triple negative breast cancer (TNBC) and it may be a potential target for TNBC diagnosis and drug design. (29581274)


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