TDP-43 (C-terminal) Antibody 12892-1-AP

Tested Applications

Applications:

ELISA, chip, IF, IHC, IP, WB
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A549 cells (shcontrol and shRNA of TDP43) were subjected to SDS PAGE followed by western blot with 12892-1-AP (TDP43 antibody) at dilution of 1:1000. (Data provided by Angran Biotech (www.miRNAlab.com)).

Immunohistochemistry of paraffin-embedded human gliomas tissue slide using 12892-1-AP( TDP-43 (C-terminal) antibody at dilution of 1:1000 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HeLa cells using 12892-1-AP(TDP-43 (C-terminal) antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Immunohistochemistry of paraffin-embedded human gliomas tissue slide using 12892-1-AP( TDP-43 (C-terminal) antibody at dilution of 1:1000 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

IP Result of anti-TARDBP (IP:12892-1-AP, 3ug; Detection:12892-1-AP 1:1000) with HeLa cells lysate 3000ug.

Species specificity:

human, mouse, rat, monkey, zebrafish

Positive WB detected in:

A549 cells, A2780 cells, C6 cells, HeLa cells, human heart tissue, human placenta tissue, K-562 cells, mouse heart tissue, mouse pancreas tissue

Positive IP detected in:

HeLa cells, mouse brain tissue

Positive IHC detected in:

human gliomas tissue, human gliomas tissue, human lung cancer tissue, mouse brain tissue

Positive IF detected in:

HeLa cells

Recommended dilution:

WB : 1:500-1:1000

IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB

IHC : 1:100-1:2000

IF : 1:50-1:500

Product Information

Source:

Rabbit

Purification method:

Antigen affinity purification

Isotype:

IgG

Storage:

PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20^oC. Aliquoting is unnecessary for -20^oC storage.

Immunogen Information

Immunogen:

TDP-43 (C-terminal) fusion protein Ag4003

Full name:

TAR DNA binding protein

Calculated molecular weight:

43 kDa

Observed molecular weight:

43-45 kDa,35 kDa

GenBank accession number:

BC001487

Gene ID (NCBI):

23435

Gene symbol

TARDBP

Synonyms

ALS10, TAR DNA binding protein, TAR DNA binding protein 43, TARDBP, TDP 43, TDP43

Background

Transactivation response (TAR) DNA-binding protein of 43 kDa (also known as TARDBP or TDP-43) was first isolated as a transcriptional inactivator binding to the TAR DNA element of the HIV-1 virus. Neumann et al. (2006) found that a hyperphosphorylated, ubiquitinated, and cleaved form of TARDBP, known as pathologic TDP-43, is the major component of the tau-negative and ubiquitin-positive inclusions that characterize amyotrophic lateral sclerosis (ALS) and the most common pathological subtype of frontotemporal lobar degeneration (FTLD-U). 12892-1-AP is a rabbit polyclonal antibody raised against the C-terminal amino acids of human TDP-43. This antibody recognizes the cleavage product of 20-30 kDa in addition to the native and phosphorylated forms of TDP-43. Immunohistochemical analyses of TDP-43 using this antibody detect both normal diffuse nuclear staining and insoluble inclusions in pathologic tissues.

Showing 1- [6] of [63] Publications

Author:		Hiroaki Suzuki
Pubmed ID:		25378556
Journal:		Hum Mol Genet
Title:		Nuclear TDP-43 causes neuronal toxicity by escaping from the inhibitory regulation by hnRNPs.
Application:		IF, WB
KD validated
Species:		human

Author:		Tassa K Saldi
Pubmed ID:		25391662
Journal:		EMBO J
Title:		TDP-1, the Caenorhabditis elegans ortholog of TDP-43, limits the accumulation of double-stranded RNA.
Application:		IF
KD validated
Species:		human

Author:		Dara Ditsworth
Pubmed ID:		28357566
Journal:		Acta Neuropathol
Title:		Mutant TDP-43 within motor neurons drives disease onset but not progression in amyotrophic lateral sclerosis.
Application:		WB
KO validated
Species:		mouse

Author:		Lindsay A Becker
Pubmed ID:		28405022
Journal:		Nature
Title:		Therapeutic reduction of ataxin-2 extends lifespan and reduces pathology in TDP-43 mice.
Application:		WB,IF

Species:		mouse

Author:		Martin H Schludi
Pubmed ID:		28409281
Journal:		Acta Neuropathol
Title:		Spinal poly-GA inclusions in a C9orf72 mouse model trigger motor deficits and inflammation without neuron loss.
Application:

Species:		human

Author:		Peter E A Ash
Pubmed ID:		28476168
Journal:		Mol Neurodegener
Title:		Dioxins and related environmental contaminants increase TDP-43 levels.
Application:		WB

Species:		human

WB Figures

A2780 cells were subjected to SDS PAGE followed by western blot with 12892-1-AP(TARDBP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours

C6 cells were subjected to SDS PAGE followed by western blot with 12892-1-AP( TARDBP Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours

HeLa cells were subjected to SDS PAGE followed by western blot with 12892-1-AP(TARDBP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

human heart tissue were subjected to SDS PAGE followed by western blot with 12892-1-AP(TARDBP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours

human placenta tissue were subjected to SDS PAGE followed by western blot with 12892-1-AP(TARDBP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

K-562 cells were subjected to SDS PAGE followed by western blot with 12892-1-AP(TARDBP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

mouse heart tissue were subjected to SDS PAGE followed by western blot with 12892-1-AP(TARDBP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours

mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 12892-1-AP(TARDBP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

IHC Figures

Immunohistochemical of paraffin-embedded human lung cancer using 12892-1-AP(TARDBP antibody) at dilution of 1:100 (under 10x lens)

Immunohistochemical of paraffin-embedded human lung cancer using 12892-1-AP(TARDBP antibody) at dilution of 1:100 (under 40x lens)

Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 12892-1-AP( TDP-43 (C-terminal) antibody at dilution of 1:1000 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 12892-1-AP( TDP-43 (C-terminal) antibody at dilution of 1:1000 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

IP Figures

IP Result of anti-TARDBP (IP:12892-1-AP, 3ug; Detection:12892-1-AP 1:1000) with HeLa cells lysate 3000ug.

IP Result of anti-TDP-43 (C-terminal) (IP:12892-1-AP, 4ug; Detection:12892-1-AP 1:1000) with mouse brain tissue lysate 3000ug.

IF Figures

Immunofluorescent analysis of HeLa cells using 12892-1-AP(TDP-43 (C-terminal) antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

TDP-43 (C-terminal) Antibody 63 Publications

Rabbit Polyclonal| Catalog number: 12892-1-AP

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Tested Applications

Product Information

Immunogen Information

Background

Showing 1- [6] of [63] Publications

WB Figures

IHC Figures

IP Figures

IF Figures

TDP-43 (C-terminal) Antibody 63 Publications

Rabbit Polyclonal| Catalog number: 12892-1-AP

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Tested Applications

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Immunogen Information

Background