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  • Featured Product
  • KD/KO Validated

TDP-43 (C-terminal) Polyclonal antibody

TDP-43 (C-terminal) Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (4)

Applications

WB, IP, IHC, IF, CoIP, chIP, ELISA

Conjugate

Unconjugated

Cat no : 12892-1-AP

Synonyms

ALS10, TAR DNA binding protein, TAR DNA binding protein 43, TDP 43, TDP43, TDP-43 (C-terminal)

Validation Data Gallery

Applications:
  • WB analysis of A549 cells using 12892-1-AP

    WB analysis of A549 cells using 12892-1-AP

    A549 cells (shcontrol and shRNA of TDP43) were subjected to SDS PAGE followed by western blot with 12892-1-AP (TDP43 antibody) at dilution of 1:1000.

  • WB analysis of HeLa using 12892-1-AP

    WB analysis of HeLa using 12892-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12892-1-AP (TDP-43 (C-terminal) antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse brain using 12892-1-AP

    WB analysis of mouse brain using 12892-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 12892-1-AP (TDP-43 (C-terminal) antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • WB analysis of C6 using 12892-1-AP

    WB analysis of C6 using 12892-1-AP

    C6 cells were subjected to SDS PAGE followed by western blot with 12892-1-AP (TARDBP Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 12892-1-AP

    IP experiment of HeLa using 12892-1-AP

    IP Result of anti-TARDBP (IP:12892-1-AP, 3ug; Detection:12892-1-AP 1:1000) with HeLa cells lysate 3000ug.

  • IP experiment of mouse brain using 12892-1-AP

    IP experiment of mouse brain using 12892-1-AP

    IP Result of anti-TDP-43 (C-terminal) (IP:12892-1-AP, 4ug; Detection:12892-1-AP 1:1000) with mouse brain tissue lysate 3000ug.

  • IHC staining of mouse brain using 12892-1-AP

    IHC staining of mouse brain using 12892-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12892-1-AP (TDP-43 (C-terminal) antibody at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 12892-1-AP

    IHC staining of mouse brain using 12892-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12892-1-AP (TDP-43 (C-terminal) antibody at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 12892-1-AP

    IHC staining of human lung cancer using 12892-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 12892-1-AP (TARDBP antibody) at dilution of 1:100 (under 40x lens).

  • IHC staining of human gliomas using 12892-1-AP

    IHC staining of human gliomas using 12892-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12892-1-AP (TDP-43 (C-terminal) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 12892-1-AP

    IF Staining of HeLa using 12892-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TDP-43 (C-terminal) antibody (12892-1-AP) at dilution of 1:4000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • IF Staining of HeLa using 12892-1-AP

    IF Staining of HeLa using 12892-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TDP-43 (C-terminal) antibody (12892-1-AP) at dilution of 1:2400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • IF Staining of Neuro-2a using 12892-1-AP

    IF Staining of Neuro-2a using 12892-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using TDP-43 (C-terminal) antibody (12892-1-AP) at dilution of 1:8000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of Neuro-2a using 12892-1-AP

    IF Staining of Neuro-2a using 12892-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using TDP-43 (C-terminal) antibody (12892-1-AP) at dilution of 1:8000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inA549 cells, mouse brain tissue, HeLa cells, C6 cells
Positive IP detected inHeLa cells, mouse brain tissue
Positive IHC detected inmouse brain tissue, human lung cancer tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, Neuro-2a cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:2000-1:8000
Sample-dependent, check data in validation data gallery

Product Information

12892-1-AP targets TDP-43 (C-terminal) in WB, IP, IHC, IF, CoIP, chIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, chicken, Drosophila, monkey, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen fusion protein
Full Name TAR DNA binding protein
Calculated molecular weight 43 kDa
Observed molecular weight 43-45 kDa, 35 kDa
GenBank accession numberBC001487
Gene symbol TARDBP
Gene ID (NCBI) 23435
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Transactivation response (TAR), DNA-binding protein of 43 kDa (also known as TARDBP or TDP-43), was first isolated as a transcriptional inactivator binding to the TAR DNA element of the HIV-1 virus. Neumann et al. (2006) found that a hyperphosphorylated, ubiquitinated, and cleaved form of TARDBP, known as pathologic TDP-43, is the major component of the tau-negative and ubiquitin-positive inclusions that characterize amyotrophic lateral sclerosis (ALS) and the most common pathological subtype of frontotemporal lobar degeneration (FTLD-U). 12892-1-AP is a rabbit polyclonal antibody raised against the C-terminal amino acids of human TDP-43. This antibody recognizes the cleavage product of 20-30 kDa in addition to the native and phosphorylated forms of TDP-43. Immunohistochemical analyses of TDP-43 using this antibody detect both normal diffuse nuclear staining and insoluble inclusions in pathologic tissues. Various forms of TDP-43 exist, including 18-35 kDa of cleaved C-terminal fragments, 45-50 kDa phosphoprotein, 55 kDa glycosylated form, 75 kDa hyperphosphorylated form, and 90-300 kDa cross-linked form. (17023659,19823856,21666678,22193176)

Recently TDP-43 has been reported to be overexpressed in triple negative breast cancer (TNBC) and it may be a potential target for TNBC diagnosis and drug design. (29581274)

Protocols

Product Specific Protocols
WB protocol for TDP-43 (C-terminal) antibody 12892-1-APDownload protocol
IHC protocol for TDP-43 (C-terminal) antibody 12892-1-APDownload protocol
IF protocol for TDP-43 (C-terminal) antibody 12892-1-APDownload protocol
IP protocol for TDP-43 (C-terminal) antibody 12892-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Nature

TDP-43 loss and ALS-risk SNPs drive mis-splicing and depletion of UNC13A.

Authors - Anna-Leigh Brown
View Article
humanWB,IHC

Science

HSP70 chaperones RNA-free TDP-43 into anisotropic intranuclear liquid spherical shells.

Authors - Haiyang Yu
  • KO Validated
View Article
human,mouseIF

Nature

TDP-43 and RNA form amyloid-like myo-granules in regenerating muscle.

Authors - Thomas O Vogler
View Article
mouseWB,IF

Nature

Therapeutic reduction of ataxin-2 extends lifespan and reduces pathology in TDP-43 mice.

Authors - Lindsay A Becker
View Article
humanIF

Nat Med

Childhood amyotrophic lateral sclerosis caused by excess sphingolipid synthesis.

Authors - Payam Mohassel
View Article
IHC

Acta Neuropathol

Splicing repression is a major function of TDP-43 in motor neurons.

Authors - Aneesh Donde
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Xin (Verified Customer) (01-23-2022)

Good performance in WB (around 45 kD)

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: mouse cortex lysate
TDP-43 (C-terminal) Antibody Western Blot validation (1:2000 dilution) in mouse cortex lysate (Cat no:12892-1-AP)
FH

Azita (Verified Customer) (06-17-2021)

Immunocytochemistry labelling of (4% PFA) fixed NSC-34 cells by TDP-43 (C-terminal) Polyclonal antibody at dilution of 1:500 showed strong labelling.

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: NSC-34 cells
FH

Jacob (Verified Customer) (03-25-2021)

Good for IF staining. It worked for cells fixation with 4% PFA.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:250
  • Cell Tissue Type: Neuron
TDP-43 (C-terminal) Antibody Immunofluorescence validation (1:250 dilution) in Neuron (Cat no:12892-1-AP)
FH

David (Verified Customer) (01-13-2020)

Good for both immunofluorescence and immunoblotting. Single band in control cells in the latter, but can detect stress induced C-terminal fragments.

  • Applications: Western Blot, Immunofluorescence,
  • Primary Antibody Dilution: 1:500 (IF); 1:1000 (WB)
  • Cell Tissue Type: SH-SY5Y
FH

Alinda (Verified Customer) (02-28-2019)

Good antibody

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: SHSY5Y
FH

Noemi (Verified Customer) (01-24-2019)

really good antibody, it detects also the cleavage product of TDP43 in WB.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: human astrocytes