Name: TDP-43 (C-terminal) Rabbit Polyclonal antibody
Brand: Proteintech
SKU: 12892-1-AP
Rating: 4.50 (2 reviews)

Tested Applications

Applications:

WB, IP, IHC, IF, chIP, ELISA
Application not tested? Please see our Guarantee

Images See all

TDP-43 (C-terminal) Antibody WB A549 cells 12892-1-AP

A549 cells (shcontrol and shRNA of TDP43) were subjected to SDS PAGE followed by western blot with 12892-1-AP (TDP43 antibody) at dilution of 1:1000.

TDP-43 (C-terminal) Antibody IHC human gliomas tissue 12892-1-AP

Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12892-1-AP( TDP-43 (C-terminal) antibody at dilution of 1:1000 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

TDP-43 (C-terminal) Antibody IF HeLa cells 12892-1-AP

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HeLa cells using 12892-1-AP(TDP-43 (C-terminal) antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12892-1-AP( TDP-43 (C-terminal) antibody at dilution of 1:1000 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

TDP-43 (C-terminal) Antibody IP HeLa cells 12892-1-AP

IP Result of anti-TARDBP (IP:12892-1-AP, 3ug; Detection:12892-1-AP 1:1000) with HeLa cells lysate 3000ug.

Species specificity:

human, mouse, rat, monkey, zebrafish

Positive WB detected in:

A549 cells, A2780 cells, C6 cells, HeLa cells, human heart tissue, human placenta tissue, K-562 cells, mouse heart tissue, mouse pancreas tissue

Positive IP detected in:

HeLa cells, mouse brain tissue

Positive IHC detected in:

human gliomas tissue, human lung cancer tissue, mouse brain tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Positive IF detected in:

HeLa cells

Recommended dilution:

WB : 1:500-1:1000

IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB

IHC : 1:100-1:2000

IF : 1:50-1:500

Sample-dependent, check data in Validation tab

Product Information

Source:

Rabbit

Purification method:

Antigen affinity purification

Isotype:

IgG

Storage:

PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20^oC. Aliquoting is unnecessary for -20^oC storage.

Immunogen Information

Immunogen:

fusion protein

Full name:

TAR DNA binding protein

Calculated molecular weight:

43 kDa

Observed molecular weight:

43-45 kDa,35 kDa

GenBank accession number:

BC001487

Gene ID (NCBI):

23435

Gene symbol

TARDBP

Synonyms

ALS10, TAR DNA binding protein, TAR DNA binding protein 43, TARDBP, TDP 43, TDP43

Background

Transactivation response (TAR), DNA-binding protein of 43 kDa (also known as TARDBP or TDP-43), was first isolated as a transcriptional inactivator binding to the TAR DNA element of the HIV-1 virus. Neumann et al. (2006) found that a hyperphosphorylated, ubiquitinated, and cleaved form of TARDBP, known as pathologic TDP-43, is the major component of the tau-negative and ubiquitin-positive inclusions that characterize amyotrophic lateral sclerosis (ALS) and the most common pathological subtype of frontotemporal lobar degeneration (FTLD-U). 12892-1-AP is a rabbit polyclonal antibody raised against the C-terminal amino acids of human TDP-43. This antibody recognizes the cleavage product of 20-30 kDa in addition to the native and phosphorylated forms of TDP-43. Immunohistochemical analyses of TDP-43 using this antibody detect both normal diffuse nuclear staining and insoluble inclusions in pathologic tissues. Various forms of TDP-43 exist, including 18-35 kDa of cleaved C-terminal fragments, 45-50 kDa phosphoprotein, 55 kDa glycosylated form, 75 kDa hyperphosphorylated form, and 90-300 kDa cross-linked form. (17023659,19823856,21666678,22193176)

Recently TDP-43 has been reported to be overexpressed in triple negative breast cancer (TNBC) and it may be a potential target for TNBC diagnosis and drug design. (29581274)

Customer Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

Share your scientific insight on this antibody

TDP-43 (C-terminal) Rabbit Polyclonal antibody

Alinda (Verified Customer), 03-01-2019

Applications

Immunofluorescence,

Primary Antibody Dilution

1:2000

No image submitted with review

Cell/Tissue Type

SHSY5Y

Lot Number

Good antibody

TDP-43 (C-terminal) Rabbit Polyclonal antibody

Noemi (Verified Customer), 01-24-2019

Applications

Western Blot,

Primary Antibody Dilution

1:1000

No image submitted with review

Cell/Tissue Type

human astrocytes

Lot Number

really good antibody, it detects also the cleavage product of TDP43 in WB.

Showing 1- [6] of [86] Publications

Author:		Hiroaki Suzuki
Pubmed ID:		25378556
Journal:		Hum Mol Genet
Title:		Nuclear TDP-43 causes neuronal toxicity by escaping from the inhibitory regulation by hnRNPs.
Application:		IF, WB
KD validated
Species:		human

Author:		Tassa K Saldi
Pubmed ID:		25391662
Journal:		EMBO J
Title:		TDP-1, the Caenorhabditis elegans ortholog of TDP-43, limits the accumulation of double-stranded RNA.
Application:		IF
KD validated
Species:		human

Author:		Kai Wang
Pubmed ID:		29728763
Journal:		J Cancer Res Clin Oncol
Title:		Identification of aberrantly expressed F-box proteins in squamous-cell lung carcinoma.
Application:		WB
KD validated
Species:		human

Author:		Dara Ditsworth
Pubmed ID:		28357566
Journal:		Acta Neuropathol
Title:		Mutant TDP-43 within motor neurons drives disease onset but not progression in amyotrophic lateral sclerosis.
Application:		WB
KO validated
Species:		mouse

Author:		Lindsay A Becker
Pubmed ID:		28405022
Journal:		Nature
Title:		Therapeutic reduction of ataxin-2 extends lifespan and reduces pathology in TDP-43 mice.
Application:		WB,IF

Species:		mouse

Author:		Martin H Schludi
Pubmed ID:		28409281
Journal:		Acta Neuropathol
Title:		Spinal poly-GA inclusions in a C9orf72 mouse model trigger motor deficits and inflammation without neuron loss.
Application:

Species:		human

WB Figures

TDP-43 (C-terminal) Antibody WB A2780 cells 12892-1-AP

A2780 cells were subjected to SDS PAGE followed by western blot with 12892-1-AP(TARDBP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours

A549 cells (shcontrol and shRNA of TDP43) were subjected to SDS PAGE followed by western blot with 12892-1-AP (TDP43 antibody) at dilution of 1:1000.

TDP-43 (C-terminal) Antibody WB C6 cells 12892-1-AP

C6 cells were subjected to SDS PAGE followed by western blot with 12892-1-AP( TARDBP Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours

TDP-43 (C-terminal) Antibody WB HeLa cells 12892-1-AP

HeLa cells were subjected to SDS PAGE followed by western blot with 12892-1-AP(TARDBP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

TDP-43 (C-terminal) Antibody WB human heart tissue 12892-1-AP

human heart tissue were subjected to SDS PAGE followed by western blot with 12892-1-AP(TARDBP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours

TDP-43 (C-terminal) Antibody WB human placenta tissue 12892-1-AP

human placenta tissue were subjected to SDS PAGE followed by western blot with 12892-1-AP(TARDBP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

TDP-43 (C-terminal) Antibody WB K-562 cells 12892-1-AP

K-562 cells were subjected to SDS PAGE followed by western blot with 12892-1-AP(TARDBP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

TDP-43 (C-terminal) Antibody WB mouse heart tissue 12892-1-AP

mouse heart tissue were subjected to SDS PAGE followed by western blot with 12892-1-AP(TARDBP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours

TDP-43 (C-terminal) Antibody WB mouse pancreas tissue 12892-1-AP

mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 12892-1-AP(TARDBP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

IHC Figures

TDP-43 (C-terminal) Antibody IHC human lung cancer tissue 12892-1-AP

Immunohistochemical analysis of paraffin-embedded human lung cancer using 12892-1-AP(TARDBP antibody) at dilution of 1:100 (under 10x lens)

Immunohistochemical analysis of paraffin-embedded human lung cancer using 12892-1-AP(TARDBP antibody) at dilution of 1:100 (under 40x lens)

TDP-43 (C-terminal) Antibody IHC mouse brain tissue 12892-1-AP

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12892-1-AP( TDP-43 (C-terminal) antibody at dilution of 1:1000 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12892-1-AP( TDP-43 (C-terminal) antibody at dilution of 1:1000 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

IP Figures

IP Result of anti-TARDBP (IP:12892-1-AP, 3ug; Detection:12892-1-AP 1:1000) with HeLa cells lysate 3000ug.

TDP-43 (C-terminal) Antibody IP mouse brain tissue 12892-1-AP

IP Result of anti-TDP-43 (C-terminal) (IP:12892-1-AP, 4ug; Detection:12892-1-AP 1:1000) with mouse brain tissue lysate 3000ug.

IF Figures

Immunofluorescent analysis of HeLa cells using 12892-1-AP(TDP-43 (C-terminal) antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

TDP-43 (C-terminal) Antibody 86 Publications

Rabbit Polyclonal| Catalog number: 12892-1-AP

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Tested Applications

Product Information

Immunogen Information

Background

Customer Reviews

TDP-43 (C-terminal) Rabbit Polyclonal antibody

TDP-43 (C-terminal) Rabbit Polyclonal antibody

Showing 1- [6] of [86] Publications

WB Figures

IHC Figures

IP Figures

IF Figures

Product-specific protocols

Standard Protocols

TDP-43 (C-terminal) Antibody 86 Publications

Rabbit Polyclonal| Catalog number: 12892-1-AP

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Related Primary Antibodies

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Tested Applications

Product Information

Immunogen Information

Background

Customer Reviews

TDP-43 (C-terminal) Rabbit Polyclonal antibody

TDP-43 (C-terminal) Rabbit Polyclonal antibody