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TDP-43 (C-terminal) Polyclonal antibody
TDP-43 (C-terminal) Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (3)
Applications
WB, IP, IHC, IF, CoIP, chIP,ELISA
Conjugate
Unconjugated
111
Cat no : 12892-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | A549 cells, mouse embryo tissue, C6 cells, HeLa cells, mouse brain tissue |
Positive IP detected in | HeLa cells, mouse brain tissue |
Positive IHC detected in | mouse brain tissue, human lung cancer tissue, human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:5000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 7 publications below |
WB | See 78 publications below |
IHC | See 16 publications below |
IF | See 43 publications below |
IP | See 5 publications below |
CoIP | See 1 publications below |
ChIP | See 1 publications below |
Product Information
12892-1-AP targets TDP-43 (C-terminal) in WB, IP, IHC, IF, CoIP, chIP,ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | Drosophila, human, monkey, mouse, zebrafish |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | fusion protein |
Full Name | TAR DNA binding protein |
Calculated molecular weight | 43 kDa |
Observed molecular weight | 43-45 kDa, 35 kDa |
GenBank accession number | BC001487 |
Gene symbol | TARDBP |
Gene ID (NCBI) | 23435 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Transactivation response (TAR), DNA-binding protein of 43 kDa (also known as TARDBP or TDP-43), was first isolated as a transcriptional inactivator binding to the TAR DNA element of the HIV-1 virus. Neumann et al. (2006) found that a hyperphosphorylated, ubiquitinated, and cleaved form of TARDBP, known as pathologic TDP-43, is the major component of the tau-negative and ubiquitin-positive inclusions that characterize amyotrophic lateral sclerosis (ALS) and the most common pathological subtype of frontotemporal lobar degeneration (FTLD-U). 12892-1-AP is a rabbit polyclonal antibody raised against the C-terminal amino acids of human TDP-43. This antibody recognizes the cleavage product of 20-30 kDa in addition to the native and phosphorylated forms of TDP-43. Immunohistochemical analyses of TDP-43 using this antibody detect both normal diffuse nuclear staining and insoluble inclusions in pathologic tissues. Various forms of TDP-43 exist, including 18-35 kDa of cleaved C-terminal fragments, 45-50 kDa phosphoprotein, 55 kDa glycosylated form, 75 kDa hyperphosphorylated form, and 90-300 kDa cross-linked form. (17023659,19823856,21666678,22193176)
Recently TDP-43 has been reported to be overexpressed in triple negative breast cancer (TNBC) and it may be a potential target for TNBC diagnosis and drug design. (29581274)
Protocols
Product Specific Protocols | |
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WB protocol for TDP-43 (C-terminal) antibody 12892-1-AP | Download protocol |
IHC protocol for TDP-43 (C-terminal) antibody 12892-1-AP | Download protocol |
IF protocol for TDP-43 (C-terminal) antibody 12892-1-AP | Download protocol |
IP protocol for TDP-43 (C-terminal) antibody 12892-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Science Heterochromatin anomalies and double-stranded RNA accumulation underlie C9orf72 poly(PR) toxicity. | ||
Neuron Cytoplasmic TDP-43 De-mixing Independent of Stress Granules Drives Inhibition of Nuclear Import, Loss of Nuclear TDP-43, and Cell Death. | ||
Cell Mol Life Sci Phosphorylated and aggregated TDP-43 with seeding properties are induced upon mutant Huntingtin (mHtt) polyglutamine expression in human cellular models. | ||
Elife Chronic optogenetic induction of stress granules is cytotoxic and reveals the evolution of ALS-FTD pathology. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH David (Verified Customer) (01-13-2020) | Good for both immunofluorescence and immunoblotting. Single band in control cells in the latter, but can detect stress induced C-terminal fragments.
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FH Alinda (Verified Customer) (02-28-2019) | Good antibody
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FH Noemi (Verified Customer) (01-24-2019) | really good antibody, it detects also the cleavage product of TDP43 in WB.
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