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TDP-43 (C-terminal) Polyclonal antibody

TDP-43 (C-terminal) Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IP, IHC, IF, chIP, ELISA

Conjugate

Unconjugated

Cat no : 12892-1-AP

Synonyms

ALS10, TAR DNA binding protein, TAR DNA binding protein 43, TDP 43, TDP43



Tested Applications

Positive WB detected inA549 cells, mouse embryo tissue, C6 cells, HeLa cells, mouse brain tissue
Positive IP detected inHeLa cells, mouse brain tissue
Positive IHC detected inmouse brain tissue, human lung cancer tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

12892-1-AP targets TDP-43 (C-terminal) in WB, IP, IHC, IF, chIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity Drosophila, human, monkey, mouse, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen fusion protein
Full Name TAR DNA binding protein
Calculated molecular weight 43 kDa
Observed molecular weight 43-45 kDa, 35 kDa
GenBank accession numberBC001487
Gene symbol TARDBP
Gene ID (NCBI) 23435
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Transactivation response (TAR), DNA-binding protein of 43 kDa (also known as TARDBP or TDP-43), was first isolated as a transcriptional inactivator binding to the TAR DNA element of the HIV-1 virus. Neumann et al. (2006) found that a hyperphosphorylated, ubiquitinated, and cleaved form of TARDBP, known as pathologic TDP-43, is the major component of the tau-negative and ubiquitin-positive inclusions that characterize amyotrophic lateral sclerosis (ALS) and the most common pathological subtype of frontotemporal lobar degeneration (FTLD-U). 12892-1-AP is a rabbit polyclonal antibody raised against the C-terminal amino acids of human TDP-43. This antibody recognizes the cleavage product of 20-30 kDa in addition to the native and phosphorylated forms of TDP-43. Immunohistochemical analyses of TDP-43 using this antibody detect both normal diffuse nuclear staining and insoluble inclusions in pathologic tissues. Various forms of TDP-43 exist, including 18-35 kDa of cleaved C-terminal fragments, 45-50 kDa phosphoprotein, 55 kDa glycosylated form, 75 kDa hyperphosphorylated form, and 90-300 kDa cross-linked form. (17023659,19823856,21666678,22193176)

Recently TDP-43 has been reported to be overexpressed in triple negative breast cancer (TNBC) and it may be a potential target for TNBC diagnosis and drug design. (29581274)

Protocols

Product Specific Protocols
WB protocol for TDP-43 (C-terminal) antibody 12892-1-APDownload protocol
IHC protocol for TDP-43 (C-terminal) antibody 12892-1-APDownload protocol
IF protocol for TDP-43 (C-terminal) antibody 12892-1-APDownload protocol
IP protocol for TDP-43 (C-terminal) antibody 12892-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC,IF

Science

Heterochromatin anomalies and double-stranded RNA accumulation underlie C9orf72 poly(PR) toxicity.

Authors - Yong-Jie Zhang
humanWB

PLoS Pathog

TDP-43 proteinopathy in Theiler's murine encephalomyelitis virus infection.

Authors - Katsuhisa Masaki
humanWB

Neuron

RNA Binding Antagonizes Neurotoxic Phase Transitions of TDP-43.

Authors - Jacob R Mann
humanWB,IF

Neuron

Cytoplasmic TDP-43 De-mixing Independent of Stress Granules Drives Inhibition of Nuclear Import, Loss of Nuclear TDP-43, and Cell Death.

Authors - Fatima Gasset-Rosa
humanWB

Cell Mol Life Sci

Phosphorylated and aggregated TDP-43 with seeding properties are induced upon mutant Huntingtin (mHtt) polyglutamine expression in human cellular models.

Authors - Laurent Coudert
humanIF

Elife

Chronic optogenetic induction of stress granules is cytotoxic and reveals the evolution of ALS-FTD pathology.

Authors - Peipei Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

David (Verified Customer) (01-13-2020)

Good for both immunofluorescence and immunoblotting. Single band in control cells in the latter, but can detect stress induced C-terminal fragments.

  • Applications: Western Blot,Immunofluorescence,
  • Primary Antibody Dilution: 1:500 (IF); 1:1000 (WB)
  • Cell Tissue Type: SH-SY5Y
FH

Alinda (Verified Customer) (02-28-2019)

Good antibody

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: SHSY5Y
FH

Noemi (Verified Customer) (01-24-2019)

really good antibody, it detects also the cleavage product of TDP43 in WB.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: human astrocytes