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TDP-43 (C-terminal) Antibody 96 Publications

Rabbit Polyclonal| Catalog number: 12892-1-AP

Featured Product KD/KO validated

3 customer reviews

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Con: 61 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, chIP, ELISA
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Images See all

TDP-43 (C-terminal) Antibody IHC mouse brain tissue 12892-1-AP

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12892-1-AP( TDP-43 (C-terminal) antibody at dilution of 1:1000 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

TDP-43 (C-terminal) Antibody IF HeLa cells 12892-1-AP

Immunofluorescent analysis of ( 4% PFA) fixed HeLa cells using 12892-1-AP (TDP-43 (C-terminal) antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

TDP-43 (C-terminal) Antibody WB A549 cells 12892-1-AP

A549 cells (shcontrol and shRNA of TDP43) were subjected to SDS PAGE followed by western blot with 12892-1-AP (TDP43 antibody) at dilution of 1:1000.

TDP-43 (C-terminal) Antibody WB HeLa cells 12892-1-AP

HeLa cells were subjected to SDS PAGE followed by western blot with 12892-1-AP (TDP-43 (C-terminal) antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours

TDP-43 (C-terminal) Antibody IP HeLa cells 12892-1-AP

IP Result of anti-TARDBP (IP:12892-1-AP, 3ug; Detection:12892-1-AP 1:1000) with HeLa cells lysate 3000ug.

Species specificity:
human, mouse, rat, Drosophila, monkey, zebrafish
Positive WB detected in:
A549 cells, mouse embryo tissue, C6 cells, HeLa cells, mouse brain tissue
Positive IP detected in:
HeLa cells, mouse brain tissue
Positive IHC detected in:
mouse brain tissue, human lung cancer tissue, human gliomas tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HeLa cells
Recommended dilution:
WB : 1:500-1:5000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:500-1:2000
IF : 1:50-1:500
Sample-dependent, check data in Validation tab

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
fusion protein
Full name:
TAR DNA binding protein
Calculated molecular weight:
43 kDa
Observed molecular weight:
43-45 kDa,35 kDa
GenBank accession number:
BC001487
Gene ID (NCBI):
23435
Gene symbol
TARDBP
Synonyms
ALS10, TAR DNA binding protein, TAR DNA binding protein 43, TDP 43, TDP43

Background

Transactivation response (TAR), DNA-binding protein of 43 kDa (also known as TARDBP or TDP-43), was first isolated as a transcriptional inactivator binding to the TAR DNA element of the HIV-1 virus. Neumann et al. (2006) found that a hyperphosphorylated, ubiquitinated, and cleaved form of TARDBP, known as pathologic TDP-43, is the major component of the tau-negative and ubiquitin-positive inclusions that characterize amyotrophic lateral sclerosis (ALS) and the most common pathological subtype of frontotemporal lobar degeneration (FTLD-U). 12892-1-AP is a rabbit polyclonal antibody raised against the C-terminal amino acids of human TDP-43. This antibody recognizes the cleavage product of 20-30 kDa in addition to the native and phosphorylated forms of TDP-43. Immunohistochemical analyses of TDP-43 using this antibody detect both normal diffuse nuclear staining and insoluble inclusions in pathologic tissues. Various forms of TDP-43 exist, including 18-35 kDa of cleaved C-terminal fragments, 45-50 kDa phosphoprotein, 55 kDa glycosylated form, 75 kDa hyperphosphorylated form, and 90-300 kDa cross-linked form. (17023659,19823856,21666678,22193176)

Recently TDP-43 has been reported to be overexpressed in triple negative breast cancer (TNBC) and it may be a potential target for TNBC diagnosis and drug design. (29581274)

Customer Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

Share your scientific insight on this antibody

TDP-43 (C-terminal) Rabbit Polyclonal antibody

David (Verified Customer), 01-14-2020
Applications
Western Blot,Immunofluorescence,
Primary Antibody Dilution
1:500 (IF); 1:1000 (WB)
No image submitted with review

No image submitted with review

Cell/Tissue Type
SH-SY5Y
Lot Number
00050567
Good for both immunofluorescence and immunoblotting. Single band in control cells in the latter, but can detect stress induced C-terminal fragments.

TDP-43 (C-terminal) Rabbit Polyclonal antibody

Alinda (Verified Customer), 03-01-2019
Applications
Immunofluorescence,
Primary Antibody Dilution
1:2000
No image submitted with review

No image submitted with review

Cell/Tissue Type
SHSY5Y
Lot Number
00050567
Good antibody

TDP-43 (C-terminal) Rabbit Polyclonal antibody

Noemi (Verified Customer), 01-24-2019
Applications
Western Blot,
Primary Antibody Dilution
1:1000
No image submitted with review

No image submitted with review

Cell/Tissue Type
human astrocytes
Lot Number
really good antibody, it detects also the cleavage product of TDP43 in WB.

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