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TDP-43 (C-terminal) Polyclonal antibody
TDP-43 (C-terminal) Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Cat no : 12892-1-AP
|Positive WB detected in||A549 cells, mouse embryo tissue, C6 cells, HeLa cells, mouse brain tissue|
|Positive IP detected in||HeLa cells, mouse brain tissue|
|Positive IHC detected in||mouse brain tissue, human lung cancer tissue, human gliomas tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:5000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:500-1:2000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
12892-1-AP targets TDP-43 (C-terminal) in WB, IP, IHC, IF, chIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||Drosophila, human, monkey, mouse, zebrafish|
|Host / Isotype||Rabbit / IgG|
|Full Name||TAR DNA binding protein|
|Calculated molecular weight||43 kDa|
|Observed molecular weight||43-45 kDa, 35 kDa|
|GenBank accession number||BC001487|
|Gene ID (NCBI)||23435|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.1% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Transactivation response (TAR), DNA-binding protein of 43 kDa (also known as TARDBP or TDP-43), was first isolated as a transcriptional inactivator binding to the TAR DNA element of the HIV-1 virus. Neumann et al. (2006) found that a hyperphosphorylated, ubiquitinated, and cleaved form of TARDBP, known as pathologic TDP-43, is the major component of the tau-negative and ubiquitin-positive inclusions that characterize amyotrophic lateral sclerosis (ALS) and the most common pathological subtype of frontotemporal lobar degeneration (FTLD-U). 12892-1-AP is a rabbit polyclonal antibody raised against the C-terminal amino acids of human TDP-43. This antibody recognizes the cleavage product of 20-30 kDa in addition to the native and phosphorylated forms of TDP-43. Immunohistochemical analyses of TDP-43 using this antibody detect both normal diffuse nuclear staining and insoluble inclusions in pathologic tissues. Various forms of TDP-43 exist, including 18-35 kDa of cleaved C-terminal fragments, 45-50 kDa phosphoprotein, 55 kDa glycosylated form, 75 kDa hyperphosphorylated form, and 90-300 kDa cross-linked form. (17023659,19823856,21666678,22193176)
Recently TDP-43 has been reported to be overexpressed in triple negative breast cancer (TNBC) and it may be a potential target for TNBC diagnosis and drug design. (29581274)
|Product Specific Protocols|
|WB protocol for TDP-43 (C-terminal) antibody 12892-1-AP||Download protocol|
|IHC protocol for TDP-43 (C-terminal) antibody 12892-1-AP||Download protocol|
|IF protocol for TDP-43 (C-terminal) antibody 12892-1-AP||Download protocol|
|IP protocol for TDP-43 (C-terminal) antibody 12892-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Heterochromatin anomalies and double-stranded RNA accumulation underlie C9orf72 poly(PR) toxicity.
TDP-43 proteinopathy in Theiler's murine encephalomyelitis virus infection.
RNA Binding Antagonizes Neurotoxic Phase Transitions of TDP-43.
Cytoplasmic TDP-43 De-mixing Independent of Stress Granules Drives Inhibition of Nuclear Import, Loss of Nuclear TDP-43, and Cell Death.
Cell Mol Life Sci
Phosphorylated and aggregated TDP-43 with seeding properties are induced upon mutant Huntingtin (mHtt) polyglutamine expression in human cellular models.
Chronic optogenetic induction of stress granules is cytotoxic and reveals the evolution of ALS-FTD pathology.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
David (Verified Customer) (01-13-2020)
Good for both immunofluorescence and immunoblotting. Single band in control cells in the latter, but can detect stress induced C-terminal fragments.
Alinda (Verified Customer) (02-28-2019)
Noemi (Verified Customer) (01-24-2019)
really good antibody, it detects also the cleavage product of TDP43 in WB.