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TARS Polyclonal antibody

TARS Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14773-1-AP

Synonyms

TARS, Threonine tRNA ligase, threonyl tRNA synthetase, ThrRS

Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using TARS Polyclonal antibody (14773-1-AP)

    WB analysis of A549 using 14773-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 14773-1-AP (TARS antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using TARS Polyclonal antibody (14773-1-AP)

    WB analysis of A549 using 14773-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 14773-1-AP (TARS antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of A549 cells using TARS Polyclonal antibody (14773-1-AP)

    IP experiment of A549 using 14773-1-AP

    IP result of anti-TARS (IP:14773-1-AP, 4ug; Detection:14773-1-AP 1:500) with A549 cells lysate 2400ug.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TARS Polyclonal antibody (14773-1-AP)

    IHC staining of human breast cancer using 14773-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14773-1-AP (TARS Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TARS Polyclonal antibody (14773-1-AP)

    IHC staining of human breast cancer using 14773-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14773-1-AP (TARS Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using TARS Polyclonal antibody (14773-1-AP)

    IF Staining of MCF-7 using 14773-1-AP

    Immunofluorescent analysis of MCF-7 cells, using TARS antibody 14773-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TARS Polyclonal antibody (14773-1-AP)

    IF Staining of HeLa using 14773-1-AP

    Immunofluorescent analysis of Hela cells, using TARS antibody 14773-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TARS Polyclonal antibody (14773-1-AP)

    IF Staining of HepG2 using 14773-1-AP

    Immunofluorescent analysis of HepG2 cells, using TARS antibody 14773-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Tested Applications

Positive WB detected inA549 cells
Positive IP detected inA549 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells, Hela cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14773-1-AP targets TARS in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TARS fusion protein Ag6614
Full Name threonyl-tRNA synthetase
Calculated Molecular Weight 83 kDa
Observed Molecular Weight 66-72 kDa
GenBank Accession NumberBC000517
Gene Symbol TARS
Gene ID (NCBI) 6897
RRIDAB_2200654
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TARS (Threonyl-tRNA synthetase), also known as threonine-tRNA ligase or ThrRS, belongs to the aminoacyl-tRNA synthetase family. Aminoacyl-tRNA synthetase (aaRS) catalyzes the specific esterification of an amino acid with the 3'-terminal hydroxyl group of its cognate tRNA. Specificity of aminoacylation catalyzed by aaRSs ensures the accurate translation of the genetic code in the first step of protein synthesis (PMID: 26324710). TARS has some isoforms with the molecular mass of 83, 87 kDa and other MW.

Protocols

Product Specific Protocols
WB protocol for TARS antibody 14773-1-APDownload protocol
IHC protocol for TARS antibody 14773-1-APDownload protocol
IF protocol for TARS antibody 14773-1-APDownload protocol
IP protocol for TARS antibody 14773-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cell

Mitochondrial Threonyl-tRNA Synthetase TARS2 Is Required for Threonine-Sensitive mTORC1 Activation.

Authors - Sung-Hoon Kim
View Article
humanWB,IP

Clin Transl Sci

Screening and characterization of myositis-related autoantibodies in COVID-19 patients

Authors - Kai-Fa Teo
View Article
humanWB

Nat Commun

Comprehensive proteogenomic characterization of early duodenal cancer reveals the carcinogenesis tracks of different subtypes

Authors - Lingling Li
View Article