TFAM Antibody

TFAM Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IP, IHC, chIP, ELISA

Conjugate

Unconjugated

Cat no : 22586-1-AP

Synonyms

MtTF1, mtTFA, TCF 6, TCF6, TCF6L1, TCF6L2, TCF6L3, TFAM, Transcription factor 6, Transcription factor 6 like 2



Tested Applications

Positive WB detected inMCF7 cells, K-562 cells, HeLa cells, human testis tissue, HEK-293 cells, A431 cells, Jurkat cells, HepG2 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inmouse colon tissue, human colon cancer tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The 22586-1-AP immunogen is Fusion Protein TFAM protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity chicken, human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TFAM fusion protein Ag18299
Full Name transcription factor A, mitochondrial
Calculated molecular weight 246aa,29 kDa
Observed molecular weight 25 kDa
GenBank accession number BC126366
Gene symbol TFAM
Gene ID (NCBI) 7019
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TFAM, also named as TCF6L2, MtTF1, TCF6, TCF6L1, TCF6L3 and mtTFA, is a mitochondrial transcription factor that is a key activator of mitochondrial transcription as well as a participant in mitochondrial genome replication. It is involved in mitochondrial transcription regulation. TFAM is required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. It activates transcription by binding immediately upstream of transcriptional start sites. TFAM is able to unwind and bend DNA.

Protocols

Product Specific Protocols
WB protocol for TFAM antibody 22586-1-APDownload protocol
IHC protocol for TFAM antibody 22586-1-APDownload protocol
IP protocol for TFAM antibody 22586-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Int J Neurosci

Vascular endothelial growth factor alleviates mitochondrial dysfunction and suppression of mitochondrial biogenesis in models of Alzheimer's disease.

Authors - Xiangtian Liu
mouseWB

Biochem Biophys Res Commun

Fibroblast growth factor 19 alleviates palmitic acid-induced mitochondrial dysfunction and oxidative stress via the AMPK/PGC-1α pathway in skeletal muscle.

Authors - Ai Guo
chickenWB

Sci Rep

Hepatic Mitochondrial Oxidative Metabolism and Lipogenesis Synergistically Adapt to Mediate Healthy Embryonic-to-Neonatal Transition in Chicken.

Authors - Chaitra Surugihalli
mouseWB

Aging (Albany NY)

Folic acid delays age-related cognitive decline in senescence-accelerated mouse prone 8: alleviating telomere attrition as a potential mechanism.

Authors - Xin Lv
human,ratWB,IHC,chIP

Theranostics

SIRT1-dependent mitochondrial biogenesis supports therapeutic effects of resveratrol against neurodevelopment damage by fluoride.

Authors - Qian Zhao
mouseIHC

Cell Metab

Mitochondrial Damage and Activation of the STING Pathway Lead to Renal Inflammation and Fibrosis.

Authors - Ki Wung Chung

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Tanusree (Verified Customer) (12-18-2019)

Product worked well in WB at 1:500 dilution.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Mouse brain
No image submitted with review