TFAM Polyclonal Antibody for IHC, IP, WB, ELISA
Cat no : 22586-1-AP
MtTF1, mtTFA, TCF 6, TCF6, TCF6L1, TCF6L2, TCF6L3, TFAM, Transcription factor 6, Transcription factor 6 like 2
|Positive WB detected in||MCF7 cells, K-562 cells, HeLa cells, human testis tissue, HEK-293 cells, A431 cells, Jurkat cells, HepG2 cells|
|Positive IP detected in||HEK-293 cells|
|Positive IHC detected in||mouse colon tissue, human colon cancer tissue, human testis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:2000-1:5000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The 22586-1-AP immunogen is Fusion Protein TFAM protein expressed in E. coli.
|Tested Reactivity||human, mouse|
|Cited Reactivity||chicken, human, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||TFAM fusion protein Ag18299|
|Full Name||transcription factor A, mitochondrial|
|Calculated molecular weight||246aa,29 kDa|
|Observed molecular weight||25 kDa|
|GenBank accession number||BC126366|
|Gene ID (NCBI)||7019|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
TFAM, also named as TCF6L2, MtTF1, TCF6, TCF6L1, TCF6L3 and mtTFA, is a mitochondrial transcription factor that is a key activator of mitochondrial transcription as well as a participant in mitochondrial genome replication. It is involved in mitochondrial transcription regulation. TFAM is required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. It activates transcription by binding immediately upstream of transcriptional start sites. TFAM is able to unwind and bend DNA.
Int J Neurosci
Vascular endothelial growth factor alleviates mitochondrial dysfunction and suppression of mitochondrial biogenesis in models of Alzheimer's disease.
Biochem Biophys Res Commun
Fibroblast growth factor 19 alleviates palmitic acid-induced mitochondrial dysfunction and oxidative stress via the AMPK/PGC-1α pathway in skeletal muscle.
Hepatic Mitochondrial Oxidative Metabolism and Lipogenesis Synergistically Adapt to Mediate Healthy Embryonic-to-Neonatal Transition in Chicken.
Aging (Albany NY)
Folic acid delays age-related cognitive decline in senescence-accelerated mouse prone 8: alleviating telomere attrition as a potential mechanism.
SIRT1-dependent mitochondrial biogenesis supports therapeutic effects of resveratrol against neurodevelopment damage by fluoride.
Mitochondrial Damage and Activation of the STING Pathway Lead to Renal Inflammation and Fibrosis.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Tanusree (Verified Customer) (12-18-2019)
Product worked well in WB at 1:500 dilution.