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TGF-beta 2-Specific Polyclonal antibody

TGF-beta 2-Specific Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 19999-1-AP

Synonyms

BSC 1 cell growth inhibitor, Cetermin, G TSF, Polyergin, TGF beta 2, TGF beta2, TGFB2, TGF-beta 2, TGFβ2



Tested Applications

Positive WB detected inK-562 cells, HeLa cells, A549 cells, mouse lung tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

19999-1-AP targets TGF-beta 2-Specific in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name transforming growth factor, beta 2
Calculated molecular weight 48 kDa
Observed molecular weight 48 kDa
GenBank accession numberNM_003238
Gene symbol TGFB2
Gene ID (NCBI) 7042
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Tranforming growth factor beta-2 (TGFB2), also named G-TSF, BSC-1 cell growth inhibitor, Polyergin and Cetermin, belongs to the TGF-beta family. TGFB2 is a secreted protein and contains a 19aa signal peptides at its N-terminal. Heterodimers with TGFB1 and with TGFB2 have been found in bone. It was proved to have suppressive effects on interleukin-2 dependent T-cell growth. This antibody is TGFB2 specific not detecting other TGFBs.

Protocols

Product Specific Protocols
WB protocol for TGF-beta 2-Specific antibody 19999-1-APDownload protocol
IHC protocol for TGF-beta 2-Specific antibody 19999-1-APDownload protocol
IF protocol for TGF-beta 2-Specific antibody 19999-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

J Cell Mol Med

The role and mechanism of transforming growth factor beta 3 in human myocardial infarction-induced myocardial fibrosis.

Authors - Ke Xue
humanWB,IHC

Oncotarget

Gender differences in fibrosis remodeling in patients with long-standing persistent atrial fibrillation.

Authors - Zhi Li
humanWB

ACS Nano

Gold Nanoparticles Induce Tumor Vessel Normalization and Impair Metastasis by Inhibiting Endothelial Smad2/3 Signaling.

Authors - Na Huang
humanWB

Ann Anat

TGF-β2 is involved in the preservation of the chondrocyte phenotype under hypoxic conditions.

Authors - R Das
humanWB,IF

Cancer Res

Osteoblast-secreted factors mediate dormancy of metastatic prostate cancer in the bone via activation of the TGFβRIII-p38MAPK-pS249/T252RB pathway.

Authors - Li-Yuan Yu-Lee
  • KD Validated
humanWB

Int J Ophthalmol

EDIL3 depletion suppress epithelial-mesenchymal transition of lens epithelial cellsviatransforming growth factor β pathway.

Authors - Rui Zhang