Filter: AllWBIPIHCIF-PIF-FroIF/ICC

WB analysis using 25859-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 25859-1-AP (TH antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 25859-1-AP
IP result of anti-TH (IP:25859-1-AP, 4ug; Detection:25859-1-AP 1:20000) with mouse brain tissue lysate 2120 ug.
IHC staining of mouse brain using 25859-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25859-1-AP (TH antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 25859-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25859-1-AP (TH antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 25859-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25859-1-AP (TH antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse brain using 25859-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using TH antibody (25859-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of mouse brain using 25859-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using TH antibody (25859-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of mouse brain using 25859-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using TH antibody (25859-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of mouse brain using 25859-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using TH antibody (25859-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of rat brain using 25859-1-AP
Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded rat brain tissue using TH antibody (25859-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), MAP2 antibody (67015-1-Ig, Clone: 1C3E6, red).
IF Staining of PC-12 using 25859-1-AP
Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using TH antibody (25859-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

TH Polyclonal antibody

TH Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IF-Fro, IP, ELISA

Cat No. 25859-1-AP

Publications(184)

Reviews (4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF/ICC, IF-P, IF-Fro, IP, ELISA

Synonyms

tyrosine hydroxylase, DYT14, DYT5b, EC:1.14.16.2, TYH

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite®594

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Publications (184)
Protocols
Reviews (4)

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	rat brain tissue
Positive IP detected in	mouse brain tissue
Positive IHC detected in	mouse brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse brain tissue
Positive IF-Fro detected in	rat brain tissue
Positive IF/ICC detected in	PC-12 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
Immunofluorescence (IF)-P	IF-P : 1:200-1:800
Immunofluorescence (IF)-FRO	IF-FRO : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 107 publications below
IHC	See 62 publications below
IF	See 76 publications below
ELISA	See 1 publications below

Product Information

25859-1-AP targets TH in WB, IHC, IF/ICC, IF-P, IF-Fro, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, canine, zebrafish, gerbil
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	TH fusion protein Ag23075 Predict reactive species
Full Name	tyrosine hydroxylase
Calculated Molecular Weight	528 aa, 59 kDa
Observed Molecular Weight	55-60 kDa
GenBank Accession Number	BC104967
Gene Symbol	TH
Gene ID (NCBI)	7054
RRID	AB_2716568
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P07101
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TH(Tyrosine 3-monooxygenase) converts L-tyrosine to L-3,4-dihydroxyphenylalanine (L-DOPA), the essential and rate-limiting step to formation of dopamine and other catecholamines. TH plays an important role in the physiology of adrenergic neurons and can be used as a marker for dopaminergic and noradrenergic neurons. This protein has 6 isoforms produced by alternative splicing with the MW from 44 kDa to 58 kDa.

Protocols

Product Specific Protocols
WB protocol for TH antibody 25859-1-AP	Download protocol
IHC protocol for TH antibody 25859-1-AP	Download protocol
IF protocol for TH antibody 25859-1-AP	Download protocol
IP protocol for TH antibody 25859-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
rat	WB,IHC	Signal Transduct Target Ther Identifying genetic targets in clinical subtypes of Parkinson's disease for optimizing pharmacological treatment strategies Authors - Dewen Kong View Article
mouse	IF	Neuron Gut-induced alpha-Synuclein and Tau propagation initiate Parkinson's and Alzheimer's disease co-pathology and behavior impairments Authors - Jie Xiang View Article
mouse	WB,IF	Adv Sci (Weinh) OTUD5 Protects Dopaminergic Neurons by Promoting the Degradation of α-Synuclein in Parkinson's Disease Model Authors - Xiaomeng Song View Article
mouse	IF	Sci Adv Lamellar Schwann cells in the Pacinian corpuscle potentiate vibration perception Authors - Yuh-Tarng Chen View Article
mouse	WB	Nat Commun Imprinted lncRNA Dio3os preprograms intergenerational brown fat development and obesity resistance. Authors - Yan-Ting Chen View Article
mouse	IF	Nat Commun NAc-VTA circuit underlies emotional stress-induced anxiety-like behavior in the three-chamber vicarious social defeat stress mouse model. Authors - Guangjian Qi View Article

Reviews

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The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH Kenzo (Verified Customer) (01-05-2024)	This antibody worked well for immunolabeling on mouse brain tissue sections Applications: Immunofluorescence Primary Antibody Dilution: 1:5000 Cell Tissue Type: Mouse brain
FH Raphael (Verified Customer) (08-02-2021)	This antibody is good for western blot. The dilution at 1/1000 was too strong but at 1/2000 it works perfectly. Applications: Western Blot Primary Antibody Dilution: 1/2000 Cell Tissue Type: Rat brain TH Antibody Western Blot validation (1/2000 dilution) in Rat brain (Cat no:25859-1-AP)
FH Teresa (Verified Customer) (11-03-2017)	Standard protocol, blocked with 1% goat serum in TBS, heat-mediated antigen retrieval Applications: Immunofluorescence Primary Antibody Dilution: 1:200 Cell Tissue Type: wildtype mouse brain TH Antibody Immunofluorescence validation (1:200 dilution) in wildtype mouse brain (Cat no:25859-1-AP)
FH Teresa (Verified Customer) (10-16-2017)	Signal was strong and specific. Could probably dilute further. Applications: Immunohistochemistry Primary Antibody Dilution: 1:200 Cell Tissue Type: mouse brain TH Antibody Immunohistochemistry validation (1:200 dilution) in mouse brain (Cat no:25859-1-AP)