TH Antibody 1 Publications

Rabbit Polyclonal| Catalog number: 25859-1-AP

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Reviews(2)


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Freight/Packing

Con: 31 μg/150 μl

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Species specificity:
human, mouse, rat

Positive WB detected in:
mouse brain tissue

Positive IP detected in:
mouse brain tissue

Positive IHC detected in:
mouse brain tissue, rat brain tissue

Positive IF detected in:
SH-SY5Y cells

Recommended dilution:
WB : 1:500-1:1000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:20-1:200
IF : 1:20-1:200

Product Information


Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
tyrosine hydroxylase

Calculated molecular weight:
528aa,59 kDa

Observed molecular weight:
50-55 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol
TH

Synonyms
TH, TYH, Tyrosine 3 hydroxylase, Tyrosine 3 monooxygenase, tyrosine hydroxylase
Background

TH(Tyrosine 3-monooxygenase) converts L-tyrosine to L-3,4-dihydroxyphenylalanine (L-DOPA), the essential and rate-limiting step to formation of dopamine and other catecholamines. TH plays an important role in the physiology of adrenergic neurons and can be used as a marker for dopaminergic and noradrenergic neurons. This protein has 6 isoformsi produced by alternative splicing with the MW from 44 kDa to 58 kDa.

TH Rabbit Polyclonal antibody

Teresa Gunn, 11-03-2017

Applications
Immunofluorescence
Lot Number
00034940

Primary Antibody Dilution
1:200
Secondary Antibody Dilution
1:200

Cell/Tissue Type
wildtype mouse brain


Standard protocol, blocked with 1% goat serum in TBS, heat-mediated antigen retrieval

TH Rabbit Polyclonal antibody

Teresa Gunn, 10-16-2017

Applications
Immunohistochemistry
Lot Number
00024940

Primary Antibody Dilution
1:200

Cell/Tissue Type
mouse brain


Signal was strong and specific. Could probably dilute further.

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