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TH Antibody 13 Publications

Rabbit Polyclonal| Catalog number: 25859-1-AP

2 customer reviews

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Con: 31 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, ELISA
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Images See all

TH Antibody WB mouse brain tissue 25859-1-AP

Various lysates were subjected to SDS PAGE followed by western blot with 25859-1-AP (TH antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours

TH Antibody IP mouse brain tissue 25859-1-AP

IP result of anti-TH(IP:25859-1-AP, 4ug; Detection:25859-1-AP 1:1000) with mouse brain tissue lysate 4000 ug

TH Antibody IHC rat brain tissue 25859-1-AP

Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 25859-1-AP (TH antibody) at dilution of 1:1000 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

TH Antibody IHC rat brain tissue 25859-1-AP

Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 25859-1-AP (TH antibody) at dilution of 1:1000 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

TH Antibody IF mouse brain tissue 25859-1-AP

Immunofluorescent analysis of ( 4% PFA) fixed mouse brain tissue using 25859-1-AP (TH antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:
human, mouse, rat, dog, zebrafish
Positive WB detected in:
mouse brain tissue, rat brain tissue
Positive IP detected in:
mouse brain tissue
Positive IHC detected in:
rat brain tissue, mouse brain tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
mouse brain tissue, SH-SY5Y cells
Recommended dilution:
WB : 1:2000-1:10000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:500-1:1000
IF : 1:50-1:500
Sample-dependent, check data in Validation tab

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
TH fusion protein Ag23075
Full name:
tyrosine hydroxylase
Calculated molecular weight:
528aa,59 kDa
Observed molecular weight:
50-55 kDa
GenBank accession number:
BC104967
Gene ID (NCBI):
7054
Gene symbol
TH
Synonyms
TH, TYH, Tyrosine 3 hydroxylase, Tyrosine 3 monooxygenase, tyrosine hydroxylase

Background

TH(Tyrosine 3-monooxygenase) converts L-tyrosine to L-3,4-dihydroxyphenylalanine (L-DOPA), the essential and rate-limiting step to formation of dopamine and other catecholamines. TH plays an important role in the physiology of adrenergic neurons and can be used as a marker for dopaminergic and noradrenergic neurons. This protein has 6 isoformsi produced by alternative splicing with the MW from 44 kDa to 58 kDa.

Customer Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

Share your scientific insight on this antibody

TH Rabbit Polyclonal antibody

Teresa (Verified Customer), 11-03-2017
Applications
Immunofluorescence
Primary Antibody Dilution
1:200
Secondary Antibody Dilution
1:200
TH Antibody Immunofluorescence validation (1:200 dilution) in wildtype mouse brain (Cat no:25859-1-AP)

TH Antibody Immunofluorescence validation (1:200 dilution) in wildtype mouse brain (Cat no:25859-1-AP)

Cell/Tissue Type
wildtype mouse brain
Lot Number
00034940
Standard protocol, blocked with 1% goat serum in TBS, heat-mediated antigen retrieval

TH Rabbit Polyclonal antibody

Teresa (Verified Customer), 10-16-2017
Applications
Immunohistochemistry
Primary Antibody Dilution
1:200
TH Antibody Immunohistochemistry validation (1:200 dilution) in mouse brain (Cat no:25859-1-AP)

TH Antibody Immunohistochemistry validation (1:200 dilution) in mouse brain (Cat no:25859-1-AP)

Cell/Tissue Type
mouse brain
Lot Number
00024940
Signal was strong and specific. Could probably dilute further.

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