TH Polyclonal antibody
TH Polyclonal Antibody for IF, IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (3)
Applications
WB, IP, IHC, IF,ELISA
Conjugate
Unconjugated
Cat no : 25859-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | rat brain tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | mouse brain tissue, SH-SY5Y cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:500-1:1000 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 16 publications below |
| IHC | See 8 publications below |
| IF | See 7 publications below |
Product Information
The immunogen of 25859-1-AP is TH Fusion Protein expressed in E. coli.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | canine, Gerbil, human, mouse, rat, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | TH fusion protein Ag23075 |
| Full Name | tyrosine hydroxylase |
| Calculated molecular weight | 528 aa, 59 kDa |
| Observed molecular weight | 50-55 kDa |
| GenBank accession number | BC104967 |
| Gene symbol | TH |
| Gene ID (NCBI) | 7054 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TH(Tyrosine 3-monooxygenase) converts L-tyrosine to L-3,4-dihydroxyphenylalanine (L-DOPA), the essential and rate-limiting step to formation of dopamine and other catecholamines. TH plays an important role in the physiology of adrenergic neurons and can be used as a marker for dopaminergic and noradrenergic neurons. This protein has 6 isoforms produced by alternative splicing with the MW from 44 kDa to 58 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TH antibody 25859-1-AP | Download protocol |
| IHC protocol for TH antibody 25859-1-AP | Download protocol |
| IF protocol for TH antibody 25859-1-AP | Download protocol |
| IP protocol for TH antibody 25859-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Int J Cardiol The effects of interleukin 17A on left stellate ganglion remodeling are mediated by neuroimmune communication in normal structural hearts. | ||
Front Cell Neurosci TRH Analog, Taltirelin Protects Dopaminergic Neurons From Neurotoxicity of MPTP and Rotenone. | ||
Mol Cell Biochem Repetitive transcranial magnetic stimulation protects mice against 6-OHDA-induced Parkinson's disease symptoms by regulating brain amyloid β1-42 level. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Teresa (Verified Customer) (11-03-2017) | Standard protocol, blocked with 1% goat serum in TBS, heat-mediated antigen retrieval
 |
FH Teresa (Verified Customer) (10-16-2017) | Signal was strong and specific. Could probably dilute further.
 |
