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TH Antibody 3 Publications

Rabbit Polyclonal| Catalog number: 25859-1-AP

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Reviews(2)

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Con: 31 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, ELISA
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Images See all

TH Antibody WB mouse brain tissue 25859-1-AP

mouse brain tissue were subjected to SDS PAGE followed by western blot with 25859-1-AP (TH antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours

TH Antibody IP mouse brain tissue 25859-1-AP

IP result of anti-TH(IP:25859-1-AP, 4ug; Detection:25859-1-AP 1:1000) with mouse brain tissue lysate 4000 ug

TH Antibody IHC mouse brain tissue 25859-1-AP

Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 25859-1-AP( TH Antibody) at dilution of 1:200 (under 40x lens).

TH Antibody IHC rat brain tissue 25859-1-AP

Immunohistochemistry of paraffin-embedded rat brain tissue slide using 25859-1-AP( TH Antibody) at dilution of 1:200 (under 40x lens).

TH Antibody IF SH-SY5Y cells 25859-1-AP

Immunofluorescent analysis of SH-SY5Y cells using 25859-1-AP(TH antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:
human, mouse, rat, zebrafish
Positive WB detected in:
mouse brain tissue, rat brain tissue
Positive IP detected in:
mouse brain tissue
Positive IHC detected in:
mouse brain tissue, rat brain tissue
Positive IF detected in:
SH-SY5Y cells
Recommended dilution:
WB : 1:1000-1:4000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:20-1:200
IF : 1:20-1:200

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
TH fusion protein Ag23075
Full name:
tyrosine hydroxylase
Calculated molecular weight:
528aa,59 kDa
Observed molecular weight:
50-55 kDa
GenBank accession number:
BC104967
Gene ID (NCBI):
7054
Gene symbol
TH
Synonyms
TH, TYH, Tyrosine 3 hydroxylase, Tyrosine 3 monooxygenase, tyrosine hydroxylase

Background

TH(Tyrosine 3-monooxygenase) converts L-tyrosine to L-3,4-dihydroxyphenylalanine (L-DOPA), the essential and rate-limiting step to formation of dopamine and other catecholamines. TH plays an important role in the physiology of adrenergic neurons and can be used as a marker for dopaminergic and noradrenergic neurons. This protein has 6 isoformsi produced by alternative splicing with the MW from 44 kDa to 58 kDa.

Customer Reviews

Share your scientific insight on this antibody

TH Rabbit Polyclonal antibody

Teresa Gunn, 11-03-2017
Applications
Immunofluorescence
Lot Number
00034940
Primary Antibody Dilution
1:200
Secondary Antibody Dilution
1:200
Cell/Tissue Type
wildtype mouse brain

Standard protocol, blocked with 1% goat serum in TBS, heat-mediated antigen retrieval

TH Rabbit Polyclonal antibody

Teresa Gunn, 10-16-2017
Applications
Immunohistochemistry
Lot Number
00024940
Primary Antibody Dilution
1:200
Cell/Tissue Type
mouse brain

Signal was strong and specific. Could probably dilute further.

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