|Positive WB detected in||mouse brain tissue, rat brain tissue|
|Positive IP detected in||mouse brain tissue|
|Positive IHC detected in||rat brain tissue, mouse brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||mouse brain tissue, SH-SY5Y cells|
|Western Blot (WB)||WB : 1:2000-1:10000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:500-1:1000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 25859-1-AP is TH Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||dog, Gerbils, human, mouse, rat, zebrafish|
|Host / Isotype||Rabbit / IgG|
|Immunogen||TH fusion protein Ag23075|
|Full Name||tyrosine hydroxylase|
|Calculated molecular weight||528 aa, 59 kDa|
|Observed molecular weight||50-55 kDa|
|GenBank accession number||BC104967|
|Gene ID (NCBI)||7054|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.1% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
TH(Tyrosine 3-monooxygenase) converts L-tyrosine to L-3,4-dihydroxyphenylalanine (L-DOPA), the essential and rate-limiting step to formation of dopamine and other catecholamines. TH plays an important role in the physiology of adrenergic neurons and can be used as a marker for dopaminergic and noradrenergic neurons. This protein has 6 isoformsi produced by alternative splicing with the MW from 44 kDa to 58 kDa.
|Product Specific Protocols|
|WB protocol for TH antibody 25859-1-AP||Download protocol|
|IHC protocol for TH antibody 25859-1-AP||Download protocol|
|IF protocol for TH antibody 25859-1-AP||Download protocol|
|IP protocol for TH antibody 25859-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Teresa (Verified Customer) (11-03-2017)
Standard protocol, blocked with 1% goat serum in TBS, heat-mediated antigen retrieval
Teresa (Verified Customer) (10-16-2017)
Signal was strong and specific. Could probably dilute further.