Tested Applications
| Positive IF-P detected in | mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-25859 targets TH in IF-P applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag23075 Product name: Recombinant human TH protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 1-260 aa of BC104967 Sequence: MPTPDATTPQAKGFRRAVSELDAKQAEAIMSPRFIGRRQSLIEDARKEREAAVAAAAAAVPSEPGDPLEAVAFEEKEGKAMLNLLFSPRATKPSALSRAVKVFETFEAKIHHLETRPAQRPRAGGPHLEYFVRLEVRRGDLAALLSGVRQVSEDVRSPAGPKVPWFPRKVSELDKCHHLVTKFDPDLDLDHPGFSDQVYRQRRKLIAEIAFQYRHGDPIPRVEYTAEEIATWKEVYTTLKGLYATHACGEHLEAFALLER Predict reactive species |
| Full Name | tyrosine hydroxylase |
| Calculated Molecular Weight | 528 aa, 59 kDa |
| Observed Molecular Weight | 50-55 kDa |
| GenBank Accession Number | BC104967 |
| Gene Symbol | TH |
| Gene ID (NCBI) | 7054 |
| RRID | AB_2919206 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Excitation Laser | Blue laser (488 nm) |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P07101 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TH(Tyrosine 3-monooxygenase) converts L-tyrosine to L-3,4-dihydroxyphenylalanine (L-DOPA), the essential and rate-limiting step to formation of dopamine and other catecholamines. TH plays an important role in the physiology of adrenergic neurons and can be used as a marker for dopaminergic and noradrenergic neurons. This protein has 6 isoforms produced by alternative splicing with the MW from 44 kDa to 58 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 TH antibody CL488-25859 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
CL488-25859 is a CoraLite® Plus 488-conjugated TH (Tyrosine Hydroxylase) polyclonal antibody. It has 3 citations across three journals: Investigative Ophthalmology & Visual Science (corneal circadian biology), Development (Notch signaling in rat intestine), and Neuropsychiatric Disease and Treatment (dopaminergic neurons in post-stroke depression). The associated research fields span ophthalmology, developmental biology, and neuroscience/psychiatry. All three studies used the antibody for immunofluorescence (IF) in mouse or rat samples.
| Species | Application | Title |
|---|---|---|
Invest Ophthalmol Vis Sci Chronic Restraint Stress Misaligns Corneal Clock via β-Adrenergic and Glucocorticoid Signaling, Altering Epithelial, Neural, Immune, Metabolic States. | ||
Neuropsychiatr Dis Treat Inhibition of SK Channels in VTA Affects Dopaminergic Neurons to Improve the Depression-Like Behaviors of Post-Stroke Depression Rats |
Reviews
What customers say
One reviewer (Angela Xie) rated CL488-25859 five stars, using it for immunofluorescence and flow cytometry. She reported excellent performance in both ICC and flow cytometry applications, noting that the pre-conjugated antibody format significantly simplified her lab workflows. Overall, the feedback is positive, highlighting ease of use and reliable results across multiple assays.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Angela (Verified Customer) (07-25-2025) | Worked great for ICC and flow cytometry. The conjugated antibody format simplifies our workflows considerably!
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