CoraLite®594-conjugated TH Polyclonal antibody

TH Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-25859

Synonyms

TH, TYH, Tyrosine 3 hydroxylase, Tyrosine 3 monooxygenase, tyrosine hydroxylase

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Validation Data Gallery

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  IF Staining of mouse brain using CL594-25859

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite®594 TH antibody (CL594-25859) at dilution of 1:200.

• 

  IF Staining of mouse brain using CL594-25859

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite®594 TH antibody (CL594-25859) at dilution of 1:200.

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Tested Applications

• Positive IF detected in: mouse brain tissue

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL594-25859 is TH Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TH fusion protein Ag23075
• Full Name: tyrosine hydroxylase
• Calculated Molecular Weight: 528 aa, 59 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: BC104967
• Gene Symbol: TH
• Gene ID (NCBI): 7054
• RRID: AB_2919876
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TH(Tyrosine 3-monooxygenase) converts L-tyrosine to L-3,4-dihydroxyphenylalanine (L-DOPA), the essential and rate-limiting step to formation of dopamine and other catecholamines. TH plays an important role in the physiology of adrenergic neurons and can be used as a marker for dopaminergic and noradrenergic neurons. This protein has 6 isoforms produced by alternative splicing with the MW from 44 kDa to 58 kDa.