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THBD Monoclonal antibody

THBD Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

5A4C5

Cat no : 67831-1-Ig

Synonyms

CD141, Fetomodulin, THBD, THRM, thrombomodulin, TM

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using THBD Monoclonal antibody (67831-1-Ig)

    WB analysis using 67831-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67831-1-Ig (THBD antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

  • Western Blot (WB) analysis of human placenta tissue using THBD Monoclonal antibody (67831-1-Ig)

    WB analysis of human placenta using 67831-1-Ig

    human placenta tissue were subjected to SDS PAGE followed by western blot with 67831-1-Ig (THBD antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using THBD Monoclonal antibody (67831-1-Ig)

    IHC staining of human lung cancer using 67831-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67831-1-Ig (THBD antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using THBD Monoclonal antibody (67831-1-Ig)

    IHC staining of human lung cancer using 67831-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67831-1-Ig (THBD antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using THBD Monoclonal antibody (67831-1-Ig)

    IHC staining of human placenta using 67831-1-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67831-1-Ig (THBD antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using THBD Monoclonal antibody (67831-1-Ig)

    IHC staining of human placenta using 67831-1-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67831-1-Ig (THBD antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inA431 cells, human placenta tissue, THP-1 cells, HUVEC cells
Positive IHC detected inhuman lung cancer tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67831-1-Ig targets THBD in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen THBD fusion protein Ag5723
Full Name thrombomodulin
Calculated Molecular Weight 60 kDa
Observed Molecular Weight105 kDa
GenBank Accession NumberBC035602
Gene Symbol THBD
Gene ID (NCBI) 7056
RRIDAB_2918593
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Thrombomodulin, also known as CD141, is an endothelial cell surface glycoprotein that forms a 1:1 complex with the coagulation factor thrombin and plays an important role as a natural anticoagulant. Thrombomodulin serves to convert thrombin from a procoagulant protein into the activator for protein C. Once converted to activated protein C (APC), this protein serves as a major anticoagulant in blood (PMID: 2827310). Thrombomodulin is also located in other cells (keratinocytes, osteoblasts, macrophages,...) where it might be involved in cell differentiation or in inflammation (PMID: 9814688). In humans, thrombomodulin is encoded by the THBD gene. Mutations in this gene are a cause of thromboembolic disease, also known as inherited thrombophilia. Thrombomodulin is glycosylated and has an apparent molecular weight of 75 to 110 kDa (PMID: 1650405; 2827310).

Protocols

Product Specific Protocols
WB protocol for THBD antibody 67831-1-IgDownload protocol
IHC protocol for THBD antibody 67831-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols