CoraLite® Plus 488-conjugated THBD Monoclonal antibody

THBD Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

5A4C5

Cat no : CL488-67831

Synonyms

CD141, Fetomodulin, THBD, THRM, thrombomodulin, TM

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Validation Data Gallery

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  IF Staining of human lung cancer using CL488-67831

  Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using CoraLite® Plus 488 THBD antibody (CL488-67831, Clone: 5A4C5 ) at dilution of 1:200.

• 

  IF Staining of human lung cancer using CL488-67831

  Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using CoraLite® Plus 488 THBD antibody (CL488-67831, Clone: 5A4C5 ) at dilution of 1:200.

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Tested Applications

• Positive IF detected in: human lung cancer tissue

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Product Information

CL488-67831 targets THBD in IF applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: THBD fusion protein Ag5723
• Full Name: thrombomodulin
• Calculated molecular weight: 60 kDa
• Observed molecular weight: 105 kDa
• GenBank accession number: BC035602
• Gene symbol: THBD
• Gene ID (NCBI): 7056
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Thrombomodulin, also known as CD141, is an endothelial cell surface glycoprotein that forms a 1:1 complex with the coagulation factor thrombin and plays an important role as a natural anticoagulant. Thrombomodulin serves to convert thrombin from a procoagulant protein into the activator for protein C. Once converted to activated protein C (APC), this protein serves as a major anticoagulant in blood (PMID: 2827310). Thrombomodulin is also located in other cells (keratinocytes, osteoblasts, macrophages,...) where it might be involved in cell differentiation or in inflammation (PMID: 9814688). In humans, thrombomodulin is encoded by the THBD gene. Mutations in this gene are a cause of thromboembolic disease, also known as inherited thrombophilia. Thrombomodulin is glycosylated and has an apparent molecular weight of 75 to 110 kDa (PMID: 1650405; 2827310).