CoraLite® Plus 488-conjugated THBD Monoclonal antibody
THBD Monoclonal Antibody for IF
Host / Isotype
Mouse / IgG2b
Reactivity
Human
Applications
IF
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
5A4C5
Cat no : CL488-67831
Synonyms
Validation Data Gallery
Tested Applications
Positive IF detected in | human lung cancer tissue |
Recommended dilution
Application | Dilution |
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Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Product Information
CL488-67831 targets THBD in IF applications and shows reactivity with Human samples.
Tested Reactivity | Human |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Immunogen | THBD fusion protein Ag5723 |
Full Name | thrombomodulin |
Calculated molecular weight | 60 kDa |
Observed molecular weight | 105 kDa |
GenBank accession number | BC035602 |
Gene symbol | THBD |
Gene ID (NCBI) | 7056 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Thrombomodulin, also known as CD141, is an endothelial cell surface glycoprotein that forms a 1:1 complex with the coagulation factor thrombin and plays an important role as a natural anticoagulant. Thrombomodulin serves to convert thrombin from a procoagulant protein into the activator for protein C. Once converted to activated protein C (APC), this protein serves as a major anticoagulant in blood (PMID: 2827310). Thrombomodulin is also located in other cells (keratinocytes, osteoblasts, macrophages,...) where it might be involved in cell differentiation or in inflammation (PMID: 9814688). In humans, thrombomodulin is encoded by the THBD gene. Mutations in this gene are a cause of thromboembolic disease, also known as inherited thrombophilia. Thrombomodulin is glycosylated and has an apparent molecular weight of 75 to 110 kDa (PMID: 1650405; 2827310).
Protocols
Product Specific Protocols | |
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IF protocol for CL Plus 488 THBD antibody CL488-67831 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |