|Positive WB detected in||mouse liver tissue|
|Positive IP detected in||mouse liver tissue|
|Positive IHC detected in||human breast cancer tissue, human liver cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:100-1:600|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
13054-1-AP targets THRSP in WB, IP, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||THRSP fusion protein Ag3721|
|Full Name||thyroid hormone responsive (SPOT14 homolog, rat)|
|Calculated molecular weight||146 aa, 17 kDa|
|Observed molecular weight||20 kDa|
|GenBank accession number||BC031989|
|Gene ID (NCBI)||7069|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
THRSP (Thyroid hormone-inducible hepatic protein) is a protein that in humans is encoded by the THRSP gene. The protein encoded by this gene is similar to the gene product of S14, a rat gene whose expression is limited to liver and adipose tissue and is controlled by nutritional and hormonal factors. This gene has been shown to be expressed in liver and adipocytes, particularly in lipomatous modules. It is also found to be expressed in lipogenic breast cancers, which suggests a role in controlling tumor lipid metabolism (RefSeq). This antibody works well in IHC, WB and IF.
The serum level of a novel lipogenic protein Spot 14 was reduced in metabolic syndrome.
Serum Spot 14 concentration is negatively associated with thyroid-stimulating hormone level.
Mol Cell Endocrinol
MiR-451a attenuates free fatty acids-mediated hepatocyte steatosis by targeting the thyroid hormone responsive spot 14 gene.