• Featured Product
  • KD/KO Validated

THRSP Polyclonal antibody

THRSP Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC,ELISA

Conjugate

Unconjugated

Cat no : 13054-1-AP

Synonyms

S14, Spot 14 protein, SPOT14, THRSP



Tested Applications

Positive WB detected inmouse liver tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman breast cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:100-1:600
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

13054-1-AP targets THRSP in WB, IP, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen THRSP fusion protein Ag3721
Full Name thyroid hormone responsive (SPOT14 homolog, rat)
Calculated molecular weight 146 aa, 17 kDa
Observed molecular weight 20 kDa
GenBank accession numberBC031989
Gene symbol THRSP
Gene ID (NCBI) 7069
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

THRSP (Thyroid hormone-inducible hepatic protein)  is a protein that in humans is encoded by the THRSP gene. The protein encoded by this gene is similar to the gene product of S14, a rat gene whose expression is limited to liver and adipose tissue and is controlled by nutritional and hormonal factors. This gene has been shown to be expressed in liver and adipocytes, particularly in lipomatous modules. It is also found to be expressed in lipogenic breast cancers, which suggests a role in controlling tumor lipid metabolism (RefSeq). This antibody works well in IHC, WB and IF.

Protocols

Product Specific Protocols
WB protocol for THRSP antibody 13054-1-APDownload protocol
IHC protocol for THRSP antibody 13054-1-APDownload protocol
IP protocol for THRSP antibody 13054-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

PLoS One

The serum level of a novel lipogenic protein Spot 14 was reduced in metabolic syndrome.

Authors - Yen-Ting Chen

Medicine (Baltimore)

Serum Spot 14 concentration is negatively associated with thyroid-stimulating hormone level.

Authors - Yen-Ting Chen
mouseWB

Mol Cell Endocrinol

MiR-451a attenuates free fatty acids-mediated hepatocyte steatosis by targeting the thyroid hormone responsive spot 14 gene.

Authors - Ni Zeng
  • KD Validated