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TIMM50 Polyclonal antibody

TIMM50 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, RIP, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 22229-1-AP

Synonyms

TIM50, TIM50L, TIMM50



Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, rat kidney tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman kidney tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 22229-1-AP is TIMM50 Fusion Protein expressed in E. coli.

Tested Reactivity human, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TIMM50 fusion protein Ag17574
Full Name translocase of inner mitochondrial membrane 50 homolog (S. cerevisiae)
Calculated molecular weight 456 aa, 50 kDa
Observed molecular weight 35-40 kDa
GenBank accession numberBC121147
Gene symbol TIMM50
Gene ID (NCBI) 92609
Conjugate Unconjugated
Form Liquid
Purification Method Antigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TIMM50 antibody 22229-1-APDownload protocol
IHC protocol for TIMM50 antibody 22229-1-APDownload protocol
IF protocol for TIMM50 antibody 22229-1-APDownload protocol
IP protocol for TIMM50 antibody 22229-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,RIP

Cell Prolif

Long non-coding RNA LOC100133669 promotes cell proliferation in oesophageal squamous cell carcinoma.

Authors - Zhuzhu Guan
  • KD Validated