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TMEM70 Polyclonal antibody

TMEM70 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20388-1-AP

Synonyms

TMEM70, transmembrane protein 70



Tested Applications

Positive WB detected inA2780 cells, Jurkat cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20388-1-AP targets TMEM70 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TMEM70 fusion protein Ag14226
Full Name transmembrane protein 70
Calculated Molecular Weight 260 aa, 29 kDa
Observed Molecular Weight 18 kDa
GenBank Accession NumberBC002748
Gene Symbol TMEM70
Gene ID (NCBI) 54968
RRIDAB_10694436
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TMEM70 belongs to the TMEM70 family. It is involved in biogenesis of mitochondrial ATP synthase. Defects in TMEM70 are a cause of mitochondrial encephalocardiomyopathy neonatal due to ATP synthase deficiency (MT-ATPSD). A publication(PMID:21147908) identifies that TMEM70 gene defect as a pan-ethnic disorder and further redefines it as the most common cause of nuclear-origin ATP synthase deficiency.

Protocols

Product Specific Protocols
WB protocol for TMEM70 antibody 20388-1-APDownload protocol
IHC protocol for TMEM70 antibody 20388-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mitochondrion

Mitochondrial membrane assembly of TMEM70 protein.

Authors - Hana Kratochvílová
humanWB

Neurogenetics

TMEM70: a mutational hot spot in nuclear ATP synthase deficiency with a pivotal role in complex V biogenesis.

Authors - Torraco Alessandra A
humanWB

JIMD Rep

Common and Novel TMEM70 Mutations in a Cohort of Italian Patients with Mitochondrial Encephalocardiomyopathy.

Authors - Daria Diodato
humanWB

Life Sci Alliance

Mitochondrial stress response triggered by defects in protein synthesis quality control.

Authors - Uwe Richter
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