TNF alpha Antibody 20 Publications

Mouse Monoclonal| Catalog number: 60291-1-Ig |CloneNo.: 7B8A11

Featured Product KD/KO validated

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Freight/Packing

Con: 222 μg/150 μl

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Species specificity:
human, mouse, rat

Positive WB detected in:
LPS treated RAW 264.7 cells, HeLa cells

Positive IHC detected in:
human breast cancer tissue, human breast cancer tissue, human colon cancer tissue, human liver cancer tissue

Recommended dilution:
WB : 1:1000-1:4000
IHC : 1:200-1:1000

Product Information


Source:
Mouse

Purification method:
Protein A purification

Isotype:
IgG2b

Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
tumor necrosis factor (TNF superfamily, member 2)

Calculated molecular weight:
233aa,26 kDa

Observed molecular weight:
17-22 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol
TNF

Synonyms
Cachectin, DIF, TNF, TNF a, TNF alpha, TNFA, TNF-a, TNFSF2, TNFα, Tumor necrosis factor
Background

TNF, as also known as TNF-alpha, or cachectin, is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. It is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-alpha converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.


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