- Featured Product
- KD/KO Validated
TNFR2 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Cat no : 19272-1-AP
CD120b, Etanercept, p75, p75TNFR, p80 TNF alpha receptor, TBP 2, TBPII, TNF R II, TNF R2, TNF R75, TNF Receptor 2, TNF Receptor II, TNF RII, TNFBR, TNFR II, TNFR1B, TNFR2, TNFR80, TNFRSF1B
Validation Data Gallery
|Positive WB detected in||Jurkat cells, HEK-293 cells, MCF-7 cells, mouse thymus tissue|
|Positive IP detected in||HEK-293 cells|
|Positive IHC detected in||human spleen tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||Hela cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
The 19272-1-AP immunogen is Fusion Protein TNFR2 protein expressed in E. coli.
|Cited Species||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||TNFR2 fusion protein Ag5866|
|Full Name||tumor necrosis factor receptor superfamily, member 1B|
|Calculated molecular weight||48 kDa|
|Observed molecular weight||75 kDa|
|GenBank accession number||BC052977|
|Gene ID (NCBI)||7133|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Tumor necrosis factor-alpha (TNFA/TNFSF2) is a multifunctional cytokine that plays a key role in regulating inflammation, immune functions, host defense, and apoptosis (PMID: 16407280). TNFA signals through two distinct cell surface receptors, TNFR1 (TNFRSF1A, CD120a, p55) and TNFR2 (TNFRSF1B, CD120b, p75). TNFR1 is widely expressed, whereas TNFR2 exhibits more restricted expression, being found on CD4 and CD8 T lymphocytes, endothelial cells, microglia, oligodendrocytes, neuron subtypes, cardiac myocytes, thymocytes and human mesenchymal stem cells (PMID: 20489699; 22374304). In contrast to TNFR1, TNFR2 does not have a death domain. TNFR2 only signals for antiapoptotic reactions. However, recent evidence indicates that TNFR2 also signals to induce TRAF2 degradation (PMID: 22374304). Various defects in the TNFR2 pathway, due to polymorphisms in the TNFR2 gene, upregulated expression of TNFR2 and TNFR2 shedding, have been implicated in the pathology of several autoimmune disorders (PMID: 20489699).
|Product Specific Protocols|
|WB protocol for TNFR2 antibody 19272-1-AP||Download protocol|
|IHC protocol for TNFR2 antibody 19272-1-AP||Download protocol|
|IF protocol for TNFR2 antibody 19272-1-AP||Download protocol|
|IP protocol for TNFR2 antibody 19272-1-AP||Download protocol|
|FC protocol for TNFR2 antibody 19272-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Expression of tumor necrosis factor receptor 2 in human non-small cell lung cancer and its role as a potential prognostic biomarker.
BMC Mol Cell Biol
EphB4/ TNFR2/ERK/MAPK signaling pathway comprises a signaling axis to mediate the positive effect of TNF-α on osteogenic differentiation.
High expression of tumor necrosis factor receptor 2 in tissue is associated with progression and prognosis of esophageal squamous cell carcinoma.
Tumor necrosis factor receptor 2 promotes growth of colorectal cancer via the PI3K/AKT signaling pathway.
Clinical significance of tumor necrosis factor receptor 2 in middle and lower thoracic esophageal squamous cell carcinoma.
Tumor necrosis factor receptor 2 may promote the proliferation and drug resistance of Kapras299 and L428 lymphoma cells via the AKT and WNT/β-catenin signaling pathways.