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TOM70 Polyclonal antibody

TOM70 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 14528-1-AP

Synonyms

TOM70, TOMM70A



Tested Applications

Positive WB detected inmouse liver tissue, mouse heart tissue, mouse skeletal muscle tissue, MCF-7 cells, mouse brain tissue, HeLa cells, mouse liver mitochondria tissue, rat heart tissue, rat skeletal muscle tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman heart tissue, human kidney tissue, mouse heart tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, Hela cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

14528-1-AP targets TOM70 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TOM70 fusion protein Ag6027
Full Name translocase of outer mitochondrial membrane 70 homolog A (S. cerevisiae)
Calculated molecular weight 67 kDa
Observed molecular weight 67 kDa
GenBank accession numberBC052994
Gene symbol TOMM70A
Gene ID (NCBI) 9868
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TOMM70A (or TOM70) is a mitochondrial import receptor responsible for the import of cytosol synthesized precursor proteins into the mitochondria. Tom70 is a member of the tetratricopeptide repeat (TPR) co-chaperone family and contains a conserved TPR clamp domain for interaction with Hsc70 and Hsp90. Recently overexpression of TOM70 has been linked to chemoresistance to drugs in breast cancer cell lines. It is also believed that Tom70 is significantly associated with breast cancer relapse and may predict the recurrence of ovarian cancer.

Protocols

Product Specific Protocols
WB protocol for TOM70 antibody 14528-1-APDownload protocol
IHC protocol for TOM70 antibody 14528-1-APDownload protocol
IF protocol for TOM70 antibody 14528-1-APDownload protocol
IP protocol for TOM70 antibody 14528-1-APDownload protocol
FC protocol for TOM70 antibody 14528-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

JCI Insight

Mitophagy and mitochondrial biogenesis in atrial tissue of patients undergoing heart surgery with cardiopulmonary bypass.

Authors - Allen M Andres
mouseIF

Am J Physiol Cell Physiol

Yap regulates mitochondrial structure remodeling during myoblast differentiation.

Authors - Shiyuan Huang
humanWB

PLoS One

Presequence-Independent Mitochondrial Import of DNA Ligase Facilitates Establishment of Cell Lines with Reduced mtDNA Copy Number.

Authors - Domenico Spadafora
humanWB

J Physiol Sci

EAE-induced upregulation of mitochondrial MnSOD is associated with increases of mitochondrial SGK1 and Tom20 protein in the mouse kidney cortex.

Authors - Sharanpreet Hira
mouseWB

Am J Physiol Cell Physiol

LonP1 regulates mitochondrial network remodeling through the PINK1/Parkin pathway during myoblast differentiation.

Authors - Shiyuan Huang
humanWB

J Hum Genet

Mutations in TOMM70 lead to multi-OXPHOS deficiencies and cause severe anemia, lactic acidosis, and developmental delay.

Authors - Xiujuan Wei

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Daniela (Verified Customer) (09-10-2019)

Used for Western Blot on total cell lysate from HeLa, this antibody identifies a strong band slightly below 75 kDa (proteins run on 15% acrylamide gel) and several fainter no-specific bands. Used for co-immunoprecipitation to detect over-expressed Flag-tagged protein interacting with Tom70, it identifies a sharp single band at the expected MW.

  • Applications: Western Blot,Immunoprecipitation,Cell culture,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HeLa cells