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  • Featured Product
  • KD/KO Validated

TOM70 Polyclonal antibody

TOM70 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 14528-1-AP

Synonyms

TOM70, TOMM70A

Validation Data Gallery

Applications:
  • Western Blot (WB) analysis of MCF-7 cells using TOM70 Polyclonal antibody (14528-1-AP)

    WB analysis of MCF-7 using 14528-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 14528-1-AP (TOM70 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using TOM70 Polyclonal antibody (14528-1-AP)

    WB analysis of HeLa using 14528-1-AP

    WB result of TOM70 antibody (14528-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TOM70 transfected HeLa cells.

  • Western Blot (WB) analysis of rat heart tissue using TOM70 Polyclonal antibody (14528-1-AP)

    WB analysis of rat heart using 14528-1-AP

    rat heart tissue were subjected to SDS PAGE followed by western blot with 14528-1-AP (TOM70 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat skeletal muscle tissue using TOM70 Polyclonal antibody (14528-1-AP)

    WB analysis of rat skeletal muscle using 14528-1-AP

    rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14528-1-AP (TOM70 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using TOM70 Polyclonal antibody (14528-1-AP)

    IP experiment of mouse brain using 14528-1-AP

    IP Result of anti-TOM70 (IP:14528-1-AP, 3ug; Detection:14528-1-AP 1:500) with mouse brain tissue lysate 6000ug.

  • Immunohistochemistry (IHC) staining of human heart tissue using TOM70 Polyclonal antibody (14528-1-AP)

    IHC staining of human heart using 14528-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 14528-1-AP (TOM70 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using TOM70 Polyclonal antibody (14528-1-AP)

    IHC staining of human heart using 14528-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 14528-1-AP (TOM70 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using TOM70 Polyclonal antibody (14528-1-AP)

    IHC staining of human liver using 14528-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 14528-1-AP (TOM70 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using TOM70 Polyclonal antibody (14528-1-AP)

    IHC staining of human liver using 14528-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 14528-1-AP (TOM70 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of mouse heart tissue using TOM70 Polyclonal antibody (14528-1-AP)

    IHC staining of mouse heart using 14528-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 14528-1-AP (TOM70 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse heart tissue using TOM70 Polyclonal antibody (14528-1-AP)

    IHC staining of mouse heart using 14528-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 14528-1-AP (TOM70 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using TOM70 Polyclonal antibody (14528-1-AP)

    IHC staining of human kidney using 14528-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14528-1-AP (TOM70 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using TOM70 Polyclonal antibody (14528-1-AP)

    IHC staining of human kidney using 14528-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14528-1-AP (TOM70 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TOM70 Polyclonal antibody (14528-1-AP)

    IF Staining of HepG2 using 14528-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TOM70 antibody (14528-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HeLa cells using TOM70 Polyclonal antibody (14528-1-AP)

    FC experiment of HeLa using 14528-1-AP

    1X10^6 HeLa cells were stained with .2ug TOM70 antibody (14528-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). FITC-Goat anti-Rabbit IgG with dilution 1:100.

Tested Applications

Positive WB detected inMCF-7 cells, HeLa cells, rat heart tissue, rat skeletal muscle tissue, mouse heart tissue, mouse brain tissue, mouse liver tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman heart tissue, human kidney tissue, mouse heart tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

14528-1-AP targets TOM70 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TOM70 fusion protein Ag6027
Full Name translocase of outer mitochondrial membrane 70 homolog A (S. cerevisiae)
Calculated molecular weight 67 kDa
Observed molecular weight 67 kDa
GenBank accession numberBC052994
Gene symbol TOMM70A
Gene ID (NCBI) 9868
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TOMM70A (or TOM70) is a mitochondrial import receptor responsible for the import of cytosol synthesized precursor proteins into the mitochondria. Tom70 is a member of the tetratricopeptide repeat (TPR) co-chaperone family and contains a conserved TPR clamp domain for interaction with Hsc70 and Hsp90. Recently overexpression of TOM70 has been linked to chemoresistance to drugs in breast cancer cell lines. It is also believed that Tom70 is significantly associated with breast cancer relapse and may predict the recurrence of ovarian cancer.

Protocols

Product Specific Protocols
WB protocol for TOM70 antibody 14528-1-APDownload protocol
IHC protocol for TOM70 antibody 14528-1-APDownload protocol
IF protocol for TOM70 antibody 14528-1-APDownload protocol
IP protocol for TOM70 antibody 14528-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nature

The ADP/ATP translocase drives mitophagy independent of nucleotide exchange.

Authors - Atsushi Hoshino
View Article
humanWB

Nature

In vivo imaging of mitochondrial membrane potential in non-small-cell lung cancer.

Authors - Milica Momcilovic
View Article
humanWB

Nature

Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.

Authors - Sarraf Shireen A SA
View Article
humanWB

Nature

The mitochondrial deubiquitinase USP30 opposes parkin-mediated mitophagy.

Authors - Baris Bingol
View Article
WB

Nat Biotechnol

Efficient proximity labeling in living cells and organisms with TurboID.

Authors - Tess C Branon
View Article
humanWB

Nat Methods

Directed evolution of APEX2 for electron microscopy and proximity labeling.

Authors - Stephanie S Lam
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Tom (Verified Customer) (04-15-2021)

Cells firxed with 3.7% paraformaldehyde. Antibody visualized using anti-rabbit Alexa Fluor 647 secondary.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Cos7
TOM70 Antibody Immunofluorescence validation (1:100 dilution) in Cos7 (Cat no:14528-1-AP)
FH

Daniela (Verified Customer) (09-10-2019)

Used for Western Blot on total cell lysate from HeLa, this antibody identifies a strong band slightly below 75 kDa (proteins run on 15% acrylamide gel) and several fainter no-specific bands.Used for co-immunoprecipitation to detect over-expressed Flag-tagged protein interacting with Tom70, it identifies a sharp single band at the expected MW.

  • Applications: Western Blot, Immunoprecipitation, Cell culture,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HeLa cells