CoraLite® Plus 488-conjugated TOM70 Polyclonal antibody

• Featured Product
• KD/KO Validated

TOM70 Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-14528

Synonyms

FLJ90470, KIAA0719, KIAA0719, TOM70, TOM70, TOMM70A

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Validation Data Gallery

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  FC experiment of HepG2 using CL488-14528

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human TOM70 (CL488-14528) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive FC detected in: HepG2 cells

Recommended dilution

• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-14528 targets TOM70 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TOM70 fusion protein Ag6027
• Full Name: translocase of outer mitochondrial membrane 70 homolog A (S. cerevisiae)
• Calculated Molecular Weight: 67 kDa
• GenBank Accession Number: BC052994
• Gene Symbol: TOMM70A
• Gene ID (NCBI): 9868
• RRID: AB_2919095
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TOMM70A (or TOM70) is a mitochondrial import receptor responsible for the import of cytosol synthesized precursor proteins into the mitochondria. Tom70 is a member of the tetratricopeptide repeat (TPR) co-chaperone family and contains a conserved TPR clamp domain for interaction with Hsc70 and Hsp90. Recently overexpression of TOM70 has been linked to chemoresistance to drugs in breast cancer cell lines. It is also believed that Tom70 is significantly associated with breast cancer relapse and may predict the recurrence of ovarian cancer.

Related Products

Flow Cytometry Perm Buffer (10X)

PF00011-C