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TPI1 Polyclonal antibody

TPI1 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 10713-1-AP

Synonyms

TIM, TPI, TPI1, Triose phosphate isomerase, Triosephosphate isomerase, triosephosphate isomerase 1



Tested Applications

Positive WB detected inHEK-293 cells, mouse liver tissue, A431 cells, HepG2 cells, mouse kidney tissue, rat kidney tissue, RAW 264.7 cells, rat liver tissue, NIH/3T3 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10713-1-AP targets TPI1 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity Drosophila, human, mouse, pig, rat, Yeast
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TPI1 fusion protein Ag1175
Full Name triosephosphate isomerase 1
Calculated molecular weight 27 kDa
Observed molecular weight 27 kDa
GenBank accession numberBC007086
Gene symbol TPI1
Gene ID (NCBI) 7167
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TPI1(Triosephosphate isomerase) is also named as TIM, TPI and belongs to the triosephosphate isomerase family. It is a key enzyme in cell metabolism that controls the glycolytic flow and energy production through the interconversion of dihydroxyacetone phosphate (DHAP) and D-glyceraldehyde 3-phosphate (G3P)(PMID:19251756). Defects in TPI1 are the cause of triosephosphate isomerase deficiency (TPI deficiency). It can exsit as a homodimer(PMID:18562316).

Protocols

Product Specific Protocols
WB protocol for TPI1 antibody 10713-1-APDownload protocol
IHC protocol for TPI1 antibody 10713-1-APDownload protocol
IP protocol for TPI1 antibody 10713-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Life Sci Alliance

Silica-based solid-phase extraction of cross-linked nucleic acid-bound proteins.

Authors - Claudio Asencio
WB

Nat Metab

An enolase inhibitor for the targeted treatment of ENO1-deleted cancers.

Authors - Yu-Hsi Lin
YeastWB

DNA Cell Biol

The Shape of Vesicle-Containing Organelles Is Critical for Their Functions in Vesicle Endocytosis.

Authors - Chelsea M Winters
humanWB

J Neurogenet

A mutation in Drosophila Aldolase causes temperature-sensitive paralysis, shortened lifespan, and neurodegeneration.

Authors - Miller Daniel D
yeastWB

Proteome Sci

Glucose induces rapid changes in the secretome of Saccharomyces cerevisiae.

Authors - Bennett J Giardina
DrosophilaWB

Brain Behav

A novel Drosophila SOD2 mutant demonstrates a role for mitochondrial ROS in neurodevelopment and disease.

Authors - Celotto Alicia M AM