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TRIM9 Polyclonal antibody

TRIM9 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC,ELISA

Conjugate

Unconjugated

Cat no : 10786-1-AP

Synonyms

KIAA0282, RING finger protein 91, RNF91, SPRING, TRIM9, tripartite motif containing 9



Tested Applications

Positive WB detected inmouse brain tissue, HepG2 cells, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inrat brain tissue, human prostate cancer tissue, rat cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10786-1-AP targets TRIM9 in WB, IP, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRIM9 fusion protein Ag1238
Full Name tripartite motif-containing 9
Calculated molecular weight 80 kDa
Observed molecular weight 79 kDa, 90 kDa, 61 kDa
GenBank accession numberBC013414
Gene symbol TRIM9
Gene ID (NCBI) 114088
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TRIM9(E3 ubiquitin-protein ligase TRIM9) is also named as RNF91 and belongs to the TRIM/RBCC family. TRIM9 protein is a brain-specific E3 ubiquitin ligase. Importantly, TRIM9 is present in LBs found in DLB and PD, suggesting that TRIM9 not only plays roles in the regulation of neuronal functions, but also participates in the formation or breakdown of abnormal inclusions through its ligase activity and besides the striatum and hippocampus, the highest expression of TRIM9 protein is seen in the frontal, temporal and occipital cortices through the western blot (PMID:20085810). It has been reported that TRIM9 is highly expressed in the cerebral cortex in mouse and human brains and that TRIM9 is decreased in damaged brains in patients who have Parkinson disease and dementia with Lewy bodies(PMID:22337885). It has 3 isoforms with molecular mass of 79, 90 and 61 kDa.

Protocols

Product Specific Protocols
WB protocol for TRIM9 antibody 10786-1-APDownload protocol
IHC protocol for TRIM9 antibody 10786-1-APDownload protocol
IP protocol for TRIM9 antibody 10786-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Springerplus

Promoter methylation of TRIM9 as a marker for detection of circulating tumor DNA in breast cancer patients.

Authors - Chieko Mishima
human, mouse, ratWB

Neurobiol Dis

TRIM9, a novel brain-specific E3 ubiquitin ligase, is repressed in the brain of Parkinson's disease and dementia with Lewy bodies.

Authors - Tanji Kunikazu K
humanWB

Cell Syst

A Multi-network Approach Identifies Protein-Specific Co-expression in Asymptomatic and Symptomatic Alzheimer's Disease.

Authors - Nicholas T Seyfried
humanWB

Cell Res

PMID: 26915459

Authors - Yunfei Qin
  • KD Validated