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TRPT1 Polyclonal antibody

TRPT1 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15541-1-AP

Synonyms

tRNA 2 phosphotransferase 1, tRNA phosphotransferase 1, TRPT1

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TRPT1 Polyclonal antibody (15541-1-AP)

    IHC staining of human liver cancer using 15541-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15541-1-AP (TRPT1 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TRPT1 Polyclonal antibody (15541-1-AP)

    IHC staining of human liver cancer using 15541-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15541-1-AP (TRPT1 Antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

15541-1-AP targets TRPT1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityrat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRPT1 fusion protein Ag7897
Full Name tRNA phosphotransferase 1
Calculated Molecular Weight 28 kDa
GenBank Accession NumberBC005133
Gene Symbol TRPT1
Gene ID (NCBI) 83707
RRIDAB_2878149
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRPT1 catalyzes the last step of tRNA splicing, the transfer of the splice junction 2'-phosphate from ligated tRNA to NAD to produce ADP-ribose 1''-2'' cyclic phosphate. It has four isoforms with MW 22-24 and 27-29 kDa.

Protocols

Product Specific Protocols
IHC protocol for TRPT1 antibody 15541-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Int J Mol Sci

Impaired 26S Proteasome Assembly Precedes Neuronal Loss in Mutant UBQLN2 Rats.

Authors - Wenjuan Zhang
View Article