TRUB1 Polyclonal antibody

TRUB1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12520-1-AP

Synonyms

PUS4, TRUB1



Tested Applications

Positive WB detected inmouse liver tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

12520-1-AP targets TRUB1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRUB1 fusion protein Ag3213
Full Name TruB pseudouridine (psi) synthase homolog 1 (E. coli)
Calculated molecular weight 349 aa, 37 kDa
Observed molecular weight 37 kDa
GenBank accession numberBC030601
Gene symbol TRUB1
Gene ID (NCBI) 142940
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TRUB1 gene encodes a 349-amino acid product, with a VFAVHKPKGPTSA box in positions 71-83 corresponding to motif I of the TruB family (probably involved in conserving protein structure). This protein may be responsible for synthesis of pseudouridine from uracil in transfer RNAs.

Protocols

Product Specific Protocols
WB protocol for TRUB1 antibody 12520-1-APDownload protocol
IHC protocol for TRUB1 antibody 12520-1-APDownload protocol
IP protocol for TRUB1 antibody 12520-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols