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  • KD/KO Validated

TSG101 Antibody

TSG101 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC, IF, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat no : 14497-1-AP

Synonyms

ESCRT I complex subunit TSG101, TSG10, TSG101, tumor susceptibility gene 101, VPS23



Tested Applications

Positive WB detected inNIH/3T3 cells, HeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman colon cancer tissue, human heart tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

14497-1-AP targets TSG101 in FC, IF, IHC, IP, WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Reactivity human, mouse, rat
Cited Species human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TSG101 fusion protein Ag5920
Full Name tumor susceptibility gene 101
Calculated molecular weight 44 kDa
Observed molecular weight 46 kDa
GenBank accession number BC002487
Gene symbol TSG101
Gene ID (NCBI) 7251
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20oC. Aliquoting is unnecessary for -20oC storage.

Background Information

TSG101(Tumor susceptibility gene 101 protein) is essential for endosomal sorting, membrane receptor degradation and the final stages of cytokinesis. It plays a crucial role for cell proliferation and cell survival. TSG101 has been identified as a candidate tumor suppressor gene and belongs to the ubiquitin-conjugating enzyme family. TSG101 is a marker for exosome. This protein has 2 isoforms produced by alternative splicing with the molecular mass of 44 and 32 kDa.

Protocols

Product Specific Protocols
WB protocol for TSG101 antibody 14497-1-APDownload protocol
IHC protocol for TSG101 antibody 14497-1-APDownload protocol
IF protocol for TSG101 antibody 14497-1-APDownload protocol
IP protocol for TSG101 antibody 14497-1-APDownload protocol
FC protocol for TSG101 antibody 14497-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

EBioMedicine

A GBM-like V-ATPase signature directs cell-cell tumor signaling and reprogramming via large oncosomes.

Authors - Irene Bertolini
WB

Int J Biochem Cell Biol

Exosomes from hypoxia-treated human adipose-derived mesenchymal stem cells enhance angiogenesis through VEGF/VEGF-R.

Authors - Yudi Han
ratWB

Stem Cells Transl Med

Impaired Bone Regenerative Effect of Exosomes Derived from Bone Marrow Mesenchymal Stem Cells in Type 1 Diabetes.

Authors - Yu Zhu
humanWB

J Biol Chem

Modulation of calreticulin expression reveals a novel exosome mediated mechanism of Z variant alpha-1 antitrypsin disposal.

Authors - Nazli Khodayari
humanWB

Int J Biol Macromol

Exosomal miR-1229 derived from colorectal cancer cells promotes angiogenesis by targeting HIPK2.

Authors - Hui-Ying Hu
humanWB

Stem Cell Res Ther

Exosomes secreted by mesenchymal stromal/stem cell-derived adipocytes promote breast cancer cell growth via activation of Hippo signaling pathway.

Authors - Shihua Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Pasquale D'Acunzo (11-06-2019)

The antibody recognize the exosomal marker TSG101 as claimed. We tested it by Western blotting in our exosome preps isolated from murine brains and it gave the signal at the correct molecular weight in the fractions that are enriched in exosomes, so it seems to be specific. However, we report here some drawbacks that we have experienced using this antibody (see the file attached): 1. There is a strong cross-reactivity of the antibody with the molecular weight marker we use (The Bio-Rad Dual Color Standard, cat. number 1610374) 2. The signal over noise ratio is a little low (the background coming from the membrane is pretty high)

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1 to 1000
  • Cell Tissue Type: Murine brain extracellular vesicles fractions
TSG101 Antibody Western Blot, validation (1 to 1000 dilution) in Murine brain extracellular vesicles fractions (Cat no:14497-1-AP)