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UCHL1/PGP9.5 Polyclonal antibody

UCHL1/PGP9.5 Polyclonal Antibody for WB, IF, FC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (4)





Cat no : 14730-1-AP


Neuron cytoplasmic protein 9.5, PARK5, PGP 9.5, PGP9.5, Ubiquitin thioesterase L1, Uch L1, UCHL1, UCH-L1, UCHL1/PGP9.5

Tested Applications

Positive WB detected inA549 cells, NCI-H1299 cells, SH-SY5Y cells, Neuro-2a cells, C6 cells, mouse brain tissue, rat brain tissue
Positive IF detected inA549 cells, mouse brain tissue
Positive FC detected inC6 cells

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14730-1-AP targets UCHL1/PGP9.5 in WB, IF, FC, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, monkey, pig, Non-human primate, canine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UCHL1/PGP9.5 fusion protein Ag6490
Full Name ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
Calculated Molecular Weight 25 kDa
Observed Molecular Weight 27 kDa
GenBank Accession NumberBC000332
Gene Symbol UCHL1
Gene ID (NCBI) 7345
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

UCHL1(Ubiquitin carboxyl-terminal hydrolase isozyme L1) is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. Expression of UCHL1 is highly specific to neurons and to cells of the diffuse neuroendocrine system and their tumors. It is present in all neurons(PMID: 6343558). This protein is present in brain at concentrations at least 50 times greater than in other organs and is a major protein component of neuronal cytoplasm (PMID:7217993). And UCHL1 is a Parkinson's disease susceptibility gene (PMID:15048890).


Product Specific Protocols
WB protocol for UCHL1/PGP9.5 antibody 14730-1-APDownload protocol
IF protocol for UCHL1/PGP9.5 antibody 14730-1-APDownload protocol
FC protocol for UCHL1/PGP9.5 antibody 14730-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




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Cell Stem Cell

Injury Induces Endogenous Reprogramming and Dedifferentiation of Neuronal Progenitors to Multipotency.

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Metabolic stress drives sympathetic neuropathy within the liver.

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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Dan (Verified Customer) (10-30-2023)

This antibody works really well and gives a positive signal on HCC1806 cells and MDAMB436 cells and shows negative expression in colorectal cancer cell lines.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: Breast cancer and colorectal cancer cell lines
UCHL1/PGP9.5 Antibody Western Blot validation (1:2000 dilution) in Breast cancer and colorectal cancer cell lines (Cat no:14730-1-AP)

Kathryn (Verified Customer) (07-10-2020)

We used this antibody for immunofluorescence on frozen mouse ovary tissues sections and it is very specific with minimal background (stained in red in the image).

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: mouse ovary
UCHL1/PGP9.5 Antibody Immunofluorescence, validation (1:100 dilution) in mouse ovary (Cat no:14730-1-AP)

Ula (Verified Customer) (11-11-2019)

The antibody produced beautiful staining in mouse skin treated with PLP fixative.

  • Applications: Immunohistochemistry,
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: paw skin
UCHL1/PGP9.5 Antibody Immunohistochemistry, validation (1:200 dilution) in paw skin (Cat no:14730-1-AP)

Rachel (Verified Customer) (07-03-2019)

This product binds with high frequency and sensitivity. I would recommend it to any researchers.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: Mouse Brain Homogenate