• Featured Product
  • KD/KO Validated

UCHL1/PGP9.5 Polyclonal antibody

UCHL1/PGP9.5 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF, CoIP

Conjugate

Unconjugated

Cat no : 14730-1-AP

Synonyms

Neuron cytoplasmic protein 9.5, PARK5, PGP 9.5, PGP9.5, Ubiquitin thioesterase L1, Uch L1, UCHL1, UCH-L1



Tested Applications

Positive WB detected inA549 cells, C6 cells, NCI-H1299 cells, mouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA549 cells, mouse brain tissue
Planning an IHC experiment? We recommend our IHCeasy UCHL1 Ready-To-Use IHC Kit. UCHL1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

14730-1-AP targets UCHL1/PGP9.5 in WB, IHC, IF, CoIP applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, monkey, mouse, Non-human primate, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UCHL1/PGP9.5 fusion protein Ag6490
Full Name ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
Calculated molecular weight 25 kDa
Observed molecular weight 27 kDa
GenBank accession numberBC000332
Gene symbol UCHL1
Gene ID (NCBI) 7345
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

UCHL1(Ubiquitin carboxyl-terminal hydrolase isozyme L1) is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. Expression of UCHL1 is highly specific to neurons and to cells of the diffuse neuroendocrine system and their tumors. It is present in all neurons(PMID: 6343558). This protein is present in brain at concentrations at least 50 times greater than in other organs and is a major protein component of neuronal cytoplasm (PMID:7217993). And UCHL1 is a Parkinson's disease susceptibility gene (PMID:15048890).

Protocols

Product Specific Protocols
WB protocol for UCHL1/PGP9.5 antibody 14730-1-APDownload protocol
IHC protocol for UCHL1/PGP9.5 antibody 14730-1-APDownload protocol
IF protocol for UCHL1/PGP9.5 antibody 14730-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratIHC

BMC Neurosci

Possible involvement of peripheral TRP channels in the hydrogen sulfide-induced hyperalgesia in diabetic rats.

Authors - José Eduardo Roa-Coria
mouseWB,IF

Aging (Albany NY)

Aging-dependent decrease in the numbers of enteric neurons, interstitial cells of Cajal and expression of connexin43 in various regions of gastrointestinal tract.

Authors - Tingyi Sun
mouse

Sci Rep

Mitochondrial uncoupling has no effect on microvascular complications in type 2 diabetes.

Authors - Lucy M Hinder
mouseWB,IF

Cell Rep

Aryl Hydrocarbon Receptor Interacting Protein Maintains Germinal Center B Cells through Suppression of BCL6 Degradation.

Authors - Dijue Sun
mouseWB,IF

Ann Clin Transl Neurol

Absence of UCHL 1 function leads to selective motor neuropathy.

Authors - Barış Genç
  • KO Validated
mouse, monkeyIF

Sci Adv

Local sympathetic innervations modulate the lung innate immune responses.

Authors - Tingting Liu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Kathryn (Verified Customer) (07-10-2020)

We used this antibody for immunofluorescence on frozen mouse ovary tissues sections and it is very specific with minimal background (stained in red in the image).

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: mouse ovary
UCHL1/PGP9.5 Antibody Immunofluorescence, validation (1:100 dilution) in mouse ovary (Cat no:14730-1-AP)
FH

Ula (Verified Customer) (11-11-2019)

The antibody produced beautiful staining in mouse skin treated with PLP fixative.

  • Applications: Immunohistochemistry,
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: paw skin
UCHL1/PGP9.5 Antibody Immunohistochemistry, validation (1:200 dilution) in paw skin (Cat no:14730-1-AP)
FH

Rachel (Verified Customer) (07-03-2019)

This product binds with high frequency and sensitivity. I would recommend it to any researchers.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: Mouse Brain Homogenate