Validation Data Gallery
|Positive WB detected in||A549 cells, C6 cells, NCI-H1299 cells, mouse brain tissue, rat brain tissue|
|Positive IHC detected in||mouse brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||A549 cells, mouse brain tissue|
|Western Blot (WB)||WB : 1:2000-1:12000|
|Immunohistochemistry (IHC)||IHC : 1:500-1:2000|
|Immunofluorescence (IF)||IF : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
14730-1-AP targets UCHL1/PGP9.5 in WB, IHC, IF, CoIP applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, monkey, mouse, Non-human primate, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||UCHL1/PGP9.5 fusion protein Ag6490|
|Full Name||ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)|
|Calculated molecular weight||25 kDa|
|Observed molecular weight||27 kDa|
|GenBank accession number||BC000332|
|Gene ID (NCBI)||7345|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
UCHL1(Ubiquitin carboxyl-terminal hydrolase isozyme L1) is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. Expression of UCHL1 is highly specific to neurons and to cells of the diffuse neuroendocrine system and their tumors. It is present in all neurons(PMID: 6343558). This protein is present in brain at concentrations at least 50 times greater than in other organs and is a major protein component of neuronal cytoplasm (PMID:7217993). And UCHL1 is a Parkinson's disease susceptibility gene (PMID:15048890).
Possible involvement of peripheral TRP channels in the hydrogen sulfide-induced hyperalgesia in diabetic rats.
Aging (Albany NY)
Aging-dependent decrease in the numbers of enteric neurons, interstitial cells of Cajal and expression of connexin43 in various regions of gastrointestinal tract.
Mitochondrial uncoupling has no effect on microvascular complications in type 2 diabetes.
Aryl Hydrocarbon Receptor Interacting Protein Maintains Germinal Center B Cells through Suppression of BCL6 Degradation.
Ann Clin Transl Neurol
Absence of UCHL 1 function leads to selective motor neuropathy.
Local sympathetic innervations modulate the lung innate immune responses.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Kathryn (Verified Customer) (07-10-2020)
We used this antibody for immunofluorescence on frozen mouse ovary tissues sections and it is very specific with minimal background (stained in red in the image).
Ula (Verified Customer) (11-11-2019)
The antibody produced beautiful staining in mouse skin treated with PLP fixative.
Rachel (Verified Customer) (07-03-2019)
This product binds with high frequency and sensitivity. I would recommend it to any researchers.