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USP32 Polyclonal antibody

USP32 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 18838-1-AP

Synonyms

Deubiquitinating enzyme 32, NY REN 60, Ubiquitin thiolesterase 32, USP10, USP32

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using USP32 Polyclonal antibody (18838-1-AP)

    WB analysis using 18838-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18838-1-AP (USP32 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using USP32 Polyclonal antibody (18838-1-AP)

    IP experiment of HeLa using 18838-1-AP

    IP result of anti-USP32 (IP:18838-1-AP, 4ug; Detection:18838-1-AP 1:500) with HeLa cells lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using USP32 Polyclonal antibody (18838-1-AP)

    IHC staining of human pancreas using 18838-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 18838-1-AP (USP32 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using USP32 Polyclonal antibody (18838-1-AP)

    IHC staining of human pancreas using 18838-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 18838-1-AP (USP32 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using USP32 Polyclonal antibody (18838-1-AP)

    IHC staining of mouse testis using 18838-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 18838-1-AP (USP32 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells
Positive IP detected inHeLa cells
Positive IHC detected inmouse testis tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18838-1-AP targets USP32 in WB, IP, IHC, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name ubiquitin specific peptidase 32
Calculated Molecular Weight 182 kDa
Observed Molecular Weight230 kDa
GenBank Accession NumberBC094680
Gene Symbol USP32
Gene ID (NCBI) 84669
RRIDAB_10598326
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

USP32 belongs to the peptidase C19 family. USP32 catalytic the reaction :Ubiquitin C-terminal thioester + H2O = ubiquitin + a thiol.

Protocols

Product Specific Protocols
WB protocol for USP32 antibody 18838-1-APDownload protocol
IHC protocol for USP32 antibody 18838-1-APDownload protocol
IP protocol for USP32 antibody 18838-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,CoIP

Acta Biochim Biophys Sin (Shanghai)

Roles of the hsa_circ_0013880/USP32/Rap1b axis in the proliferation and apoptosis of acute myeloid leukemia cells

Authors - Heyang Zhang
  • KD Validated
View Article