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VDAC1/Porin Antibody 3 Publications

Mouse Monoclonal| Catalog number: 66345-1-Ig |CloneNo.: 1E2C7

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Con: 64 μg/150 μl

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Tested Applications

Applications:
WB, IHC, ELISA
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Images See all

VDAC1/Porin Antibody WB RAW 264.7 cells 66345-1-Ig

Various lysates were subjected to SDS PAGE followed by western blot with 66345-1-Ig (VDAC1/Porin antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours

VDAC1/Porin Antibody IHC human heart tissue 66345-1-Ig

Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66345-1-Ig (VDAC1/Porin antibody) at dilution of 1:100 (under 10x lens).

VDAC1/Porin Antibody IHC human heart tissue 66345-1-Ig

Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66345-1-Ig (VDAC1/Porin antibody) at dilution of 1:100 (under 40x lens).

Species specificity:
human, mouse, rat
Positive WB detected in:
RAW 264.7 cells, HEK-293 cells, Jurkat cells, K-562 cells, C6 cells, ROS1728 cells, NIH/3T3 cells, 3T3-L1 cells
Positive IHC detected in:
human heart tissue, human skeletal muscle tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution:
WB : 1:2000-1:20000
IHC : 1:50-1:500
Sample-dependent, check data in Validation tab

Product Information

Source:
Mouse
Purification method:
Protein A purification
Isotype:
IgG3
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC.Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Peptide
Full name:
voltage-dependent anion channel 1
Calculated molecular weight:
31 kDa
Observed molecular weight:
35-37 kDa
GenBank accession number:
NM_003374
Gene ID (NCBI):
7416
Gene symbol
VDAC1
Synonyms
hVDAC1, Plasmalemmal porin, Porin, PORIN 31 HL, Porin 31HL, Porin 31HM, VDAC, VDAC 1, VDAC1

Background

VDAC1, also named as VDAC, porin 31HM, porin 31HL and plasmalemmal porin, belongs to the eukaryotic mitochondrial porin family. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV, to form a channel through the mitochondrial outer membrane and also the plasma membrane. Unlike other membrane transport proteins, porins are large enough to allow passive diffusion. Studies have shown that VDAC1 is subject to both phosphorylation and acetylation (PMID: 23233904). The apparent molecular weight of VDAC1 is 30-37 kDa (PMID: 14573604; 23754752; 25681439). Hypoxic conditions were found to trigger cleavage of the VDAC1 C-terminal to yield a 26-kDa truncated but active form (PMID: 22389449; 23233904).


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