Tested Applications
| Positive WB detected in | mouse brain tissue |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human lung cancer tissue, human testis tissue, mouse brain tissue, mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10901-1-AP targets VPS18 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1140 Product name: Recombinant human VPS18 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 226-355 aa of BC001513 Sequence: QRLFQFIGRAAEGAEAQGFSGLFAAYTDHPPPFREFPSNLGYSELAFYTPKLRSAPRAFAWMMGDGVLYGALDCGRPDSLLSEERVWEYPEGVGPGASPPLAIVLTQFHFLLLLADRVEAVCTLTGQVVL Predict reactive species |
| Full Name | vacuolar protein sorting 18 homolog (S. cerevisiae) |
| Calculated Molecular Weight | 110 kDa |
| Observed Molecular Weight | 100-110 kDa |
| GenBank Accession Number | BC001513 |
| Gene Symbol | VPS18 |
| Gene ID (NCBI) | 57617 |
| RRID | AB_2273089 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9P253 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Vps18 is a central member of Vps-C complex. Vps18 may play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for VPS18 antibody 10901-1-AP | Download protocol |
| IHC protocol for VPS18 antibody 10901-1-AP | Download protocol |
| IP protocol for VPS18 antibody 10901-1-AP | Download protocol |
| WB protocol for VPS18 antibody 10901-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The VPS18 Polyclonal antibody (Cat# 10901-1-AP) has 10 citations. They appear in journals including Autophagy (3), Nature Communications, Oncogene, Current Biology, Virulence, International Journal of Molecular Sciences, Scientific Reports, and Journal of Biological Chemistry. Research fields span autophagy and vesicle-mediated protein trafficking, endocytosis, glioblastoma, ovarian cancer, lung tumorigenesis, and viral replication.
| Species | Application | Title |
|---|---|---|
Autophagy BAG3 and SYNPO (synaptopodin) facilitate phospho-MAPT/Tau degradation via autophagy in neuronal processes. | ||
Autophagy SDC1-dependent TGM2 determines radiosensitivity in glioblastoma by coordinating EPG5-mediated fusion of autophagosomes with lysosomes | ||
Nat Commun Deacetylated SNAP47 recruits HOPS to facilitate autophagosome-lysosome fusion independent of STX17 | ||
Oncogene Complementary intra- and extracellular AGR2 activities support epithelial ovarian cancer cell aggressiveness. | ||
Curr Biol Cellular mechanotransduction relies on tension-induced and chaperone-assisted autophagy. |
