VPS18 Polyclonal antibody

VPS18 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 10901-1-AP

Synonyms

hVPS18, KIAA1475, PEP3, VPS18



Tested Applications

Positive WB detected inmouse brain tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman lung cancer tissue, mouse testis tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA549 cells
Positive FC detected inK-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 3 publications below

Product Information

10901-1-AP targets VPS18 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VPS18 fusion protein Ag1140
Full Name vacuolar protein sorting 18 homolog (S. cerevisiae)
Calculated molecular weight 110 kDa
Observed molecular weight 100-110 kDa
GenBank accession numberBC001513
Gene symbol VPS18
Gene ID (NCBI) 57617
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Vps18 is a central member of Vps-C complex. Vps18 may play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes.

Protocols

Product Specific Protocols
WB protocol for VPS18 antibody 10901-1-APDownload protocol
IHC protocol for VPS18 antibody 10901-1-APDownload protocol
IF protocol for VPS18 antibody 10901-1-APDownload protocol
IP protocol for VPS18 antibody 10901-1-APDownload protocol
FC protocol for VPS18 antibody 10901-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Autophagy

BAG3 and SYNPO (synaptopodin) facilitate phospho-MAPT/Tau degradation via autophagy in neuronal processes.

Authors - Changyi Ji
humanWB

Curr Biol

Cellular mechanotransduction relies on tension-induced and chaperone-assisted autophagy.

Authors - Anna Ulbricht
humanWB

Autophagy

Acetylation of STX17 (syntaxin 17) controls autophagosome maturation.

Authors - Qiuhong Shen