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  • Featured Product
  • KD/KO Validated

WFDC12 Polyclonal antibody

WFDC12 Polyclonal Antibody for IHC,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 25101-1-AP

Synonyms

C20orf122, SWAM2, WAP2, WFDC12, Whey acidic protein 2

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human lung cancer tissue using WFDC12 Polyclonal antibody (25101-1-AP)

    IHC staining of human lung cancer using 25101-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 25101-1-AP (WFDC12 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using WFDC12 Polyclonal antibody (25101-1-AP)

    IHC staining of human lung cancer using 25101-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 25101-1-AP (WFDC12 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using WFDC12 Polyclonal antibody (25101-1-AP)

    IHC staining of human prostate cancer using 25101-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 25101-1-AP (WFDC12 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using WFDC12 Polyclonal antibody (25101-1-AP)

    IHC staining of human prostate cancer using 25101-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 25101-1-AP (WFDC12 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive IHC detected inhuman lung cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25101-1-AP is WFDC12 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen WFDC12 fusion protein Ag18795
Full Name WAP four-disulfide core domain 12
Calculated Molecular Weight 111 aa, 12 kDa
GenBank Accession NumberBC146514
Gene Symbol WFDC12
Gene ID (NCBI) 128488
RRIDAB_2879897
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for WFDC12 antibody 25101-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

J Invest Dermatol

The Whey Acidic Protein WFDC12 Is Specifically Expressed in Terminally Differentiated Keratinocytes and Regulates Epidermal Serine Protease Activity.

Authors - Polina Kalinina
  • KD Validated
View Article
humanIHC,IF

Front Immunol

A role for whey acidic protein four-disulfide-core 12 (WFDC12) in the pathogenesis and development of psoriasis disease

Authors - Fulei Zhao
View Article
humanIHC

Cell Death Dis

WFDC12-overexpressing contributes to the development of atopic dermatitis via accelerating ALOX12/15 metabolism and PAF accumulation

Authors - Guolin Li
View Article