|Positive WB detected in||mouse liver tissue, A549 cells|
|Positive IHC detected in||human pancreas cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
12197-1-AP targets WWP2 in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||WWP2 fusion protein Ag2835|
|Full Name||WW domain containing E3 ubiquitin protein ligase 2|
|Calculated molecular weight||870 aa, 99 kDa|
|Observed molecular weight||110-115 kDa|
|GenBank accession number||BC013645|
|Gene ID (NCBI)||11060|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
WWP2(WW domain-containing protein 2) is also named as AIP2 and belongs to the NEDD4-like protein family. It functions as an E3 ubiquitin ligase for PTEN that plays a vital role in tumour-cell survival and is also involved in regulating transcription, embryonic stem-cell fate, cellular transport and T-cell activation processes(PMID:21532586).WWP2, which specifically interacted with ES cell-specific transcription factor Oct-4 and promoted its ubiquitination both in vitro and in vivo, is a ubiquitously expressed protein and exists in both cytoplasmic and nuclear compartments in ES cells(PMID:15047715).
Cell Biochem Funct
WWP2 ameliorates acute kidney injury by mediating p53 ubiquitylation and degradation.
J Cell Mol Med
WWP2 regulates SIRT1-STAT3 acetylation and phosphorylation involved in hypertensive angiopathy.
J Dent Res
WWP2 Promotes Odontoblastic Differentiation by Monoubiquitinating KLF5.
Br J Pharmacol
Costunolide represses hepatic fibrosis through WWP2-mediated Notch3 degradation.
Role of WW domain E3 ubiquitin protein ligase 2 in modulating ubiquitination and Degradation of Septin4 in oxidative stress endothelial injury.
Cell Death Differ
Selective targeting of ubiquitination and degradation of PARP1 by E3 ubiquitin ligase WWP2 regulates isoproterenol-induced cardiac remodeling.