WWP2 Polyclonal antibody

WWP2 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 12197-1-AP

Synonyms

AIP2, WW domain containing protein 2, WWP2, WWp2 like



Tested Applications

Positive WB detected inmouse liver tissue, A549 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

12197-1-AP targets WWP2 in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen WWP2 fusion protein Ag2835
Full Name WW domain containing E3 ubiquitin protein ligase 2
Calculated molecular weight 870 aa, 99 kDa
Observed molecular weight 110-115 kDa
GenBank accession numberBC013645
Gene symbol WWP2
Gene ID (NCBI) 11060
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

WWP2(WW domain-containing protein 2) is also named as AIP2 and belongs to the NEDD4-like protein family. It functions as an E3 ubiquitin ligase for PTEN that plays a vital role in tumour-cell survival and is also involved in regulating transcription, embryonic stem-cell fate, cellular transport and T-cell activation processes(PMID:21532586).WWP2, which specifically interacted with ES cell-specific transcription factor Oct-4 and promoted its ubiquitination both in vitro and in vivo, is a ubiquitously expressed protein and exists in both cytoplasmic and nuclear compartments in ES cells(PMID:15047715).

Protocols

Product Specific Protocols
WB protocol for WWP2 antibody 12197-1-APDownload protocol
IHC protocol for WWP2 antibody 12197-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Biochem Funct

WWP2 ameliorates acute kidney injury by mediating p53 ubiquitylation and degradation.

Authors - Hong Che
mouseWB

J Cell Mol Med

WWP2 regulates SIRT1-STAT3 acetylation and phosphorylation involved in hypertensive angiopathy.

Authors - Ying Zhang
mouseWB,IHC,IF

J Dent Res

WWP2 Promotes Odontoblastic Differentiation by Monoubiquitinating KLF5.

Authors - J Fu
humanWB

Br J Pharmacol

Costunolide represses hepatic fibrosis through WWP2-mediated Notch3 degradation.

Authors - Mao-Xu Ge
mouseWB

Redox Biol

Role of WW domain E3 ubiquitin protein ligase 2 in modulating ubiquitination and Degradation of Septin4 in oxidative stress endothelial injury.

Authors - Naijin Zhang
humanWB

Cell Death Differ

Selective targeting of ubiquitination and degradation of PARP1 by E3 ubiquitin ligase WWP2 regulates isoproterenol-induced cardiac remodeling.

Authors - Naijin Zhang
  • KD Validated