ZO-1 Antibody 30 Publications

Rabbit Polyclonal| Catalog number: 21773-1-AP

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Freight/Packing

Con: 56 μg/150 μl

Choose size:

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Species specificity:
human, dog, mouse, rat

Positive WB detected in:
HepG2 cells, A431 cells, COLO 320 cells, HEK-293 cells

Positive IP detected in:
HEK-293 cells

Positive IHC detected in:
human kidney tissue

Positive IF detected in:
MDCK cells, HUVEC cells

Positive FC detected in:
MCF-7 cells

Recommended dilution:
WB : 1:500-1:1000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:50-1:200
IF : 1:20-1:200

Product Information


Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
tight junction protein 1 (zona occludens 1)

Calculated molecular weight:
1748aa,195 kDa

Observed molecular weight:
230 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol
TJP1

Synonyms
Tight junction protein 1, Tight junction protein ZO 1, TJP1, ZO 1, ZO1, ZO-1, Zona occludens protein 1, Zonula occludens protein 1
Background

Tight junction (or zonula occludens) form the continuous intercellular barrier between epithelial and endothelial cells, which is required to separate tissue spaces and regulate selective movement of solutes across the epithelium and endothelium (PMID: 20066090). ZO-1 (also known as TJP1) is a peripheral membrane phosphoprotein located on the cytoplasmic face and is expressed in tight junctions of both epithelial and endothelial cells (PMID: 3528172). It binds the transmembrane proteins occludin and the claudins linking them to cytoskeletal actin (PMID: 17418867). ZO-1 belongs to a family of multidomain proteins known as the membrane-associated guanylate kinase homologs (MAGUKs). It is a pivotal tight junction protein and may be involved in signalling mechanisms regulating cell proliferation and differentiation (PMID: 22782886).


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