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ZO-1 Antibody 53 Publications

Rabbit Polyclonal| Catalog number: 21773-1-AP

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Con: 56 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, FC, ELISA
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Images See all

ZO-1 Antibody WB HepG2 cells 21773-1-AP

HepG2 cells were subjected to SDS PAGE followed by western blot with 21773-1-AP(ZO-1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

ZO-1 Antibody IF MDCK cells 21773-1-AP

Immunofluorescent analysis of ( -20℃ Ethanol ) fixed MDCK cells using 21773-1-AP(ZO-1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

ZO-1 Antibody IF HUVEC cells 21773-1-AP

Immunofluorescent analysis of ( -20°C Ethanol ) fixed HUVEC cells using 21773-1-AP(ZO-1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

ZO-1 Antibody FC MCF-7 cells 21773-1-AP

1X10^6 MCF-7 cells were stained with 0.2ug ZO-1 antibody (21773-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

ZO-1 Antibody IHC human kidney tissue 21773-1-AP

Immunohistochemical of paraffin-embedded human kidney using 21773-1-AP(ZO-1 antibody) at dilution of 1:100 (under 40x lens)

ZO-1 Antibody IHC human kidney tissue 21773-1-AP

Immunohistochemical of paraffin-embedded human kidney using 21773-1-AP(ZO-1 antibody) at dilution of 1:100 (under 10x lens)

ZO-1 Antibody IP HEK-293 cells 21773-1-AP

IP Result of anti-ZO-1 (IP:21773-1-AP, 5ug; Detection:21773-1-AP 1:800) with HEK-293 cells lysate 1800ug.

Species specificity:
human, dog, mouse, rat, hamster, cow, piglet
Positive WB detected in:
HepG2 cells, A431 cells, COLO 320 cells, HEK-293 cells
Positive IP detected in:
HEK-293 cells
Positive IHC detected in:
human kidney tissue
Positive IF detected in:
MDCK cells, CHO cells, HUVEC cells
Positive FC detected in:
MCF-7 cells
Recommended dilution:
WB : 1:500-1:1000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:50-1:200
IF : 1:20-1:200

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
ZO-1 fusion protein Ag16454
Full name:
tight junction protein 1 (zona occludens 1)
Calculated molecular weight:
1748aa,195 kDa
Observed molecular weight:
230 kDa
GenBank accession number:
BC111712
Gene ID (NCBI):
7082
Gene symbol
TJP1
Synonyms
Tight junction protein 1, Tight junction protein ZO 1, TJP1, ZO 1, ZO1, ZO-1, Zona occludens protein 1, Zonula occludens protein 1

Background

Tight junction (or zonula occludens) form the continuous intercellular barrier between epithelial and endothelial cells, which is required to separate tissue spaces and regulate the selective movement of solutes across the epithelium and endothelium (PMID: 20066090) (this part is copy paste from the article PMCID: PMC 2742087). ZO-1 (also known as TJP1) is a peripheral membrane phosphoprotein located on the cytoplasmic face and is expressed in tight junctions of both epithelial and endothelial cells (PMID: 3528172). It binds the transmembrane proteins occludin and the claudins linking them to cytoskeletal actin (PMID: 17418867). ZO-1 belongs to a family of multidomain proteins known as the membrane-associated guanylate kinase homologs (MAGUKs). It is a pivotal tight junction protein and may be involved in signaling mechanisms regulating cell proliferation and differentiation (PMID: 22782886).


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