beta Amyloid Polyclonal antibody

beta Amyloid Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse , rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27320-1-AP

Synonyms

A4, AAA, ABETA, ABPP, AD1, AICD 50, AICD 57, AICD 59, AID(50), AID(57), AID(59), Amyloid beta A4 protein, amyloid P, APP, APPI, beta Amyloid, CTFgamma, CVAP, Gamma CTF(50) C31, PN II, PN2, PreA4, Protease nexin II, S APP alpha Soluble APP beta



Tested Applications

Positive WB detected infetal human brain tissue, U-251 cells, mouse brain tissue
Positive IHC detected inmouse brain tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 27320-1-AP is beta Amyloid Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse , rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen beta Amyloid fusion protein Ag26299
Full Name amyloid beta (A4) precursor protein
Calculated molecular weight 86 aa, 9 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC004369
Gene symbol APP
Gene ID (NCBI) 351
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Aβ derives from APP via proteolytic cleavage by proteases called α-, β- and γ-secretase. The α-secretase cleavage precludes the formation of Aβ, while the β- and γ-cleavages generate APP components with amyloidogenic features. Amyloid beta A4 precursor protein(APP), encoded by APP gene which locate on human chromosome 21q, is a cell surface receptor and performs physiological functions on the surface of neurons relevant to neurite growth, neuronal adhesion and axonogenesis. APP expressed in all fetal tissues and is pronounced in brain, kidney, heart and spleen, but weak in liver. Defects in APP are the cause of Alzheimer disease type 1 (AD1). Amyloid β (Aβ) precursor protein (APP) is a 100-140 kDa transmembrane glycoprotein that exists as several isoforms. This . APP can be cleaved into several chains, this antibody could recognize N-terminal fragment of APP (N-APP).

Protocols

Product Specific Protocols
WB protocol for beta Amyloid antibody 27320-1-APDownload protocol
IHC protocol for beta Amyloid antibody 27320-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols