Phospho-TDP43 (Ser409/410) Monoclonal antibody

Phospho-TDP43 (Ser409/410) Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1A2C1

Cat no : 66318-1-Ig

Synonyms

ALS10, phospho(409/410)-TDP43, Phospho-TDP43 (Ser409/410), TAR DNA binding protein, TAR DNA binding protein 43, TDP 43, TDP43



Tested Applications

Positive WB detected inEthacrynic acid treated HeLa cells, HEK-293 cells, human placenta tissue, Neuro-2a cells, C6 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

66318-1-Ig targets Phospho-TDP43 (Ser409/410) in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name TAR DNA binding protein
Calculated molecular weight 43 kDa
Observed molecular weight 45-50 kDa, 35 kDa
GenBank accession numberNM_007375
Gene symbol TARDBP
Gene ID (NCBI) 23435
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The TARDBP gene encodes the TDP-43 protein, initially found to repress HIV-1 transcription by binding TAR DNA. TDP-43 has since been shown to bind RNA as well as DNA, and have multiple functions in transcriptional repression, translational regulation and pre-mRNA splicing. For instance, it is reported to regulate alternate splicing of the CTFR gene. In 2006 Neumann et al. found that hyperphosphorylated, ubiquitinated and/or cleaved forms of TDP-43, collectively known as pathological TDP-43, play a major role in the disease mechanisms of ubiquitin-positive, tau- and alpha-synuclein-negative frontotemporal dementia (FTLD-U) and in amyotrophic lateral sclerosis (ALS). Various forms of TDP-43 exist, including 18-35 kDa of cleaved C-terminal fragments, 45-50 kDa phospho-protein, 55 kDa glycosylated form, 75 kDa hyperphosphorylated form, and 90-300 kDa cross-linked form. (PMID: 17023659,19823856, 21666678, 22193176). 66318-1-Ig is a phospho-dependent monoclonal antibody specifically recognizing phospho-TDP43 (409/410). It does not react with native TDP-43.

Protocols

Product Specific Protocols
WB protocol for Phospho-TDP43 (Ser409/410) antibody 66318-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Neuropathology

Clinicopathological description of two cases with SQSTM1 gene mutation associated with frontotemporal dementia.

Authors - Gabor G Kovacs
mouseWB

Neurochem Res

Endoplasmic Reticulum Stress Signalling Induces Casein Kinase 1-Dependent Formation of Cytosolic TDP-43 Inclusions in Motor Neuron-Like Cells.

Authors - David A Hicks
humanWB,IF

Mol Cell Biol

The N-termini of TAR DNA-binding protein-43 (TDP43) C-terminal fragments influence degradation, aggregation propensity and morphology.

Authors - Yasar Arfat T Kasu
humanIHC

Eur J Neurol

SOD1 p.D12Y variant is associated with amyotrophic lateral sclerosis/distal myopathy spectrum.

Authors - G Tasca

PLoS Genet

Progressive ataxia of Charolais cattle highlights a role of KIF1C in sustainable myelination.

Authors - Amandine Duchesne
humanIF

Mol Cell

Stress Induces Dynamic, Cytotoxicity-Antagonizing TDP-43 Nuclear Bodies via Paraspeckle LncRNA NEAT1-Mediated Liquid-Liquid Phase Separation.

Authors - Chen Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Alejandro (Verified Customer) (04-08-2020)

Good product

  • Applications: Immunohistochemistry