Scientist II – Assay Development and Cell Culture

Proteintech is a fast-growing Biotech company located in the greater Chicago area. Our Mission is to support our customers in all their endeavors, be it accelerating life sciences research and/or clinical diagnostics and therapies.

Proteintech is an international biotechnology company that manufactures antibodies, ELISA kits, and proteins for use in life science research. Proteintech makes all its own products to the highest standards possible, with complete control over the whole manufacturing, validation, and QC process. 200,000+ citations of Proteintech products demonstrates what matters most to the company: the success of its reagents in the hands of scientists, helping them make discoveries to change and explain the world around us. 

Position Summary:

We are seeking a Scientist II with a strong background in cell biology, assay development, and cell culture  to join our team focused on the expression, purification, and functional validation of human proteins. This role will lead efforts in bioassay development, protein activity testing, and scale-up of mammalian cell culture processes. The successful candidate will bring hands-on expertise and scientific leadership to advance our pipeline of high-quality protein reagents.

Key Responsibilities:

• Design, development, and validation of cell-based and biochemical assays to assess protein bioactivity.
• Perform cell culture on various mammalian and murine cell lines
• Perform transient and stable transfections; develop and maintain stable cell lines.
• Apply Design of Experiments (DOE) for process optimization in a GMP-aligned environment.
• Use ISO 13485 standards based validation of assays
• Statistical analysis of assay results. 
• Knowledge of statistical software such as GraphPAD prism is a must
• Analyze, interpret, and summarize experimental data; prepare detailed reports and presentations.
• Maintain accurate laboratory records and ensure compliance with safety and quality standards.
• Contribute to maintaining a clean, safe, and well-organized lab environment.

Preferred Education/Experience:

• B.S  in Cell Biology, Biochemistry, Molecular Biology, or a related field with 3–5 years of industry or Academic lab experience or M.S in any life science discipline with 2-4 years of relevant experience

Knowledge, Skills, and Abilities:

• Strong foundation in cell biology, protein biochemistry, and assay development.
• Demonstrated expertise in mammalian cell culture, transfection, and protein expression.
• Familiarity with various statistical methods is a plus
• Working knowledge of GMP and international quality standards.
• Excellent organizational skills and ability to manage multiple tasks simultaneously.
• Strong communication skills—both written and verbal.
• Proficiency with data analysis tools (Excel, statistical software, etc.).
• Self-motivated, detail-oriented, and able to work independently as well as collaboratively.

Benefits:

• Paid time off
• 401K with company match
• Health, dental and vision insurance

Contact Katie Bellows katie@ptglab.com 

Proteintech group is an EEO/Affirmative Action Employer and does not discriminate on the basis of race, color, religion, sex, sexual orientation, gender identity, national origin, protected veteran status, disability or any other legally protected status.

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