c-Kit/CD117 Monoklonaler Antikörper
c-Kit/CD117 Monoklonal Antikörper für ELISA
Wirt / Isotyp
Maus / IgG2b
Getestete Reaktivität
human
Anwendung
ELISA
Konjugation
Unkonjugiert
CloneNo.
2E8G4
Kat-Nr. : 60169-1-Ig
Synonyme
Galerie der Validierungsdaten
Geprüfte Anwendungen
Empfohlene Verdünnung
| Anwendung | Verdünnung |
|---|---|
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Produktinformation
60169-1-Ig bindet in ELISA c-Kit/CD117 und zeigt Reaktivität mit human
| Getestete Reaktivität | human |
| Wirt / Isotyp | Maus / IgG2b |
| Klonalität | Monoklonal |
| Typ | Antikörper |
| Immunogen | Peptid |
| Vollständiger Name | v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog |
| Berechnetes Molekulargewicht | 110 kDa |
| Beobachtetes Molekulargewicht | 120 kDa |
| GenBank-Zugangsnummer | BC071593 |
| Gene symbol | KIT |
| Gene ID (NCBI) | 3815 |
| Konjugation | Unkonjugiert |
| Form | Liquid |
| Reinigungsmethode | Protein-G-Reinigung |
| Lagerungspuffer | PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3. |
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
KIT, also named as SCFR, c-Kit and CD117, is a transmembrane tyrosine kinase encoded by the cKit proto oncogene. It is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). KIT acts to regulate a variety of biological responses including cell proliferation, apoptosis, chemotaxis and adhesion. Ligand(SCF) binding to the extracellular domain leads to autophosphorylation on several tyrosine residues within the cytoplasmic domain, and activation. Phosphorylation at tyrosine 721 of KIT allows binding and activation of PI3 kinase. Loss of expression of KIT appears to be associated with progression of some tumors (melanoma) and autocrine/paracrine stimulation of the kit/SCF system may participate in human solid tumors such as lung, breast, testicular and gynecological malignancies. Mutations in Kit have been found to be important for tumor growth and progression in a variety of cancers including mast cell diseases, gastrointestinal stromal tumor, acute myeloid leukemia, Ewing sarcoma and lung cancer.
