|Positive WB detected in||Jurkat cells, NIH/3T3 cells|
|Positive IP detected in||Jurkat cells|
|Positive IHC detected in||human kidney tissue, human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 17670-1-AP is JAK2 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse, pig, rat, zebrafish|
|Host / Isotype||Rabbit / IgG|
|Immunogen||JAK2 fusion protein Ag11264|
|Full Name||Janus kinase 2 (a protein tyrosine kinase)|
|Calculated molecular weight||1132 aa, 130 kDa|
|Observed molecular weight||150 kDa|
|GenBank accession number||BC039695|
|Gene ID (NCBI)||3717|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Janus kinases (JAKs) are non-receptor tyrosine kinases that are activated by cytokine binding to cytokine receptors resulting in stimulation of the JAK-STAT signaling pathway. Aberrant activation of this signaling pathway is associated with numerous immune disorders and cancer. This antibody can detect full-length band of 130 kDa, and another bands 150 kDa after posttranslational modification.
Brain Res Bull
Inhibition of Connexin43 Hemichannels with Gap19 Protect-s Cerebral Ischemia/Reperfusion Injury via the JAK2/STAT3 Pathway in Mice.
FGFR2 Promotes Expression of PD-L1 in Colorectal Cancer via the JAK/STAT3 Signaling Pathway.
Colloids Surf B Biointerfaces
Toxicological exploration of peptide-based cationic liposomes in siRNA delivery.
Naunyn Schmiedebergs Arch Pharmacol
Protocatechuic aldehyde protects against isoproterenol-induced cardiac hypertrophy via inhibition of the JAK2/STAT3 signaling pathway.
Am J Physiol Renal Physiol
Triptolide delays disease progression in an adult rat model of polycystic kidney disease through the JAK2/STAT3 pathway.
Oxid Med Cell Longev
Mild Hypothermia Attenuates Hepatic Ischemia-Reperfusion Injury through Regulating the JAK2/STAT3-CPT1a-Dependent Fatty Acid β-Oxidation.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Carly (Verified Customer) (11-17-2020)
Tested using EDTA plasma on an antibody microarray