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PI3 Kinase p110 Beta Polyclonal antibody
PI3 Kinase p110 Beta Polyclonal Antibody for FC, IHC, IP, WB, ELISA
Cat no : 20584-1-AP
|Positive WB detected in
|rat liver tissue
|Positive IP detected in
|Positive IHC detected in
|human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive FC detected in
|Western Blot (WB)
|WB : 1:200-1:1000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:50-1:500
|Flow Cytometry (FC)
|FC : 0.20 ug per 10^6 cells in a 100 µl suspension
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
20584-1-AP targets PI3 Kinase p110 Beta in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, rat samples.
|human, mouse, rat, chicken, rabbit, fish, zebrafish, pig, canine, Megalobrama amblycephala
|Host / Isotype
|Rabbit / IgG
|phosphoinositide-3-kinase, catalytic, beta polypeptide
|Calculated molecular weight
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
PIK3CB(phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform) is also named as PIK3C1, PI3K-beta, p110beta. The gene encodes a 1070 amino acid protein which belongs to the PI3/PI4-kinase family. Phosphoinositide 3-kinases (PI3Ks) have been implicated as participants in signaling pathways regulating cell growth by virtue of their activation in response to various mitogenic stimuli. The class I PI3 kinases are heterodimers composed of 110 kDa catalytic subunits that associate with regulatory adaptor proteins. Four class I catalytic subunits have been identified, PIK3CA (p110α), PIK3CB (p110β), PIK3CD (p110δ) and PIK3CG (p110γ)(PMID:19177002). This antibody is specific to PIK3CB.
|Product Specific Protocols
|WB protocol for PI3 Kinase p110 Beta antibody 20584-1-AP
|IHC protocol for PI3 Kinase p110 Beta antibody 20584-1-AP
|IP protocol for PI3 Kinase p110 Beta antibody 20584-1-AP
|FC protocol for PI3 Kinase p110 Beta antibody 20584-1-AP
|Click here to view our Standard Protocols
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Macarena Lucia (Verified Customer) (10-17-2022)