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  • KD/KO Validated

PI3 Kinase p110 Beta Polyclonal antibody

PI3 Kinase p110 Beta Polyclonal Antibody for FC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat and More (8)

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 20584-1-AP

Synonyms

p110 BETA, PI3 Kinase p110 beta, PI3 kinase subunit beta, PI3K, PI3K beta, PI3K p110(beta), PI3Kbeta, PI3KCB, PIK3C1, PIK3CB, PtdIns 3 kinase subunit beta



Tested Applications

Positive WB detected inrat liver tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

20584-1-AP targets PI3 Kinase p110 Beta in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Cited Reactivityhuman, mouse, rat, chicken, rabbit, fish, zebrafish, pig, canine, Megalobrama amblycephala
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name phosphoinositide-3-kinase, catalytic, beta polypeptide
Calculated molecular weight 123 kDa
Observed molecular weight 110-130 kDa
GenBank accession numberNM_006219
Gene symbol PIK3CB
Gene ID (NCBI) 5291
RRIDAB_10734439
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PIK3CB(phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform) is also named as PIK3C1, PI3K-beta, p110beta. The gene encodes a 1070 amino acid protein which belongs to the PI3/PI4-kinase family. Phosphoinositide 3-kinases (PI3Ks) have been implicated as participants in signaling pathways regulating cell growth by virtue of their activation in response to various mitogenic stimuli. The class I PI3 kinases are heterodimers composed of 110 kDa catalytic subunits that associate with regulatory adaptor proteins. Four class I catalytic subunits have been identified, PIK3CA (p110α), PIK3CB (p110β), PIK3CD (p110δ) and PIK3CG (p110γ)(PMID:19177002). This antibody is specific to PIK3CB.

Protocols

Product Specific Protocols
WB protocol for PI3 Kinase p110 Beta antibody 20584-1-APDownload protocol
IHC protocol for PI3 Kinase p110 Beta antibody 20584-1-APDownload protocol
IP protocol for PI3 Kinase p110 Beta antibody 20584-1-APDownload protocol
FC protocol for PI3 Kinase p110 Beta antibody 20584-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human

Brain

MMP13 inhibition rescues cognitive decline in Alzheimer transgenic mice via BACE1 regulation.

Authors - Bing-Lin Zhu
mouse

Redox Biol

GSTP1-mediated S-glutathionylation of Pik3r1 is a redox hub that inhibits osteoclastogenesis through regulating autophagic flux

Authors - Xiaoxiao Ji
human

Adv Healthc Mater

A ROS-Responsive Liposomal Composite Hydrogel Integrating Improved Mitochondrial Function and Pro-Angiogenesis for Efficient Treatment of Myocardial Infarction.

Authors - Zhi Zheng
humanIHC

Clin Cancer Res

STAT3/HOTAIR signaling axis regulates HNSCC growth in an EZH2-dependent manner.

Authors - Shanshan Sun
human,rabbit

Environ Res

Effects of external environment on promoter methylation of PIK3R5 and related pathway regulation in steroid-induced femoral head necrosis

Authors - Wentao Qin
mouse

Phytomedicine

Deciphering the pharmacological mechanisms of Rostellularia procumbens (L) Nees. Extract alleviates adriamycin-induced nephropathy in vivo and in vitro

Authors - Zhongzhu Ai

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Macarena Lucia (Verified Customer) (10-17-2022)

nice band

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000