Tested Applications
| Positive WB detected in | rat liver tissue |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 ul suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
20584-1-AP targets PI3 Kinase p110 Beta in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, rat samples.
| Tested Reactivity | human, rat |
| Cited Reactivity | human, rat, pig, rabbit, canine, chicken, zebrafish, fish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | phosphoinositide-3-kinase, catalytic, beta polypeptide |
| Calculated Molecular Weight | 123 kDa |
| Observed Molecular Weight | 110-130 kDa |
| GenBank Accession Number | NM_006219 |
| Gene Symbol | PI3 Kinase p110 Beta |
| Gene ID (NCBI) | 5291 |
| RRID | AB_10734439 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P42338 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PIK3CB(phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform) is also named as PIK3C1, PI3K-beta, p110beta. The gene encodes a 1070 amino acid protein which belongs to the PI3/PI4-kinase family. Phosphoinositide 3-kinases (PI3Ks) have been implicated as participants in signaling pathways regulating cell growth by virtue of their activation in response to various mitogenic stimuli. The class I PI3 kinases are heterodimers composed of 110 kDa catalytic subunits that associate with regulatory adaptor proteins. Four class I catalytic subunits have been identified, PIK3CA (p110α), PIK3CB (p110β), PIK3CD (p110δ) and PIK3CG (p110γ)(PMID:19177002). This antibody is specific to PIK3CB.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for PI3 Kinase p110 Beta antibody 20584-1-AP | Download protocol |
| IF protocol for PI3 Kinase p110 Beta antibody 20584-1-AP | Download protocol |
| IHC protocol for PI3 Kinase p110 Beta antibody 20584-1-AP | Download protocol |
| IP protocol for PI3 Kinase p110 Beta antibody 20584-1-AP | Download protocol |
| WB protocol for PI3 Kinase p110 Beta antibody 20584-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
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| Species | Application | Title |
|---|---|---|
Mol Cancer CMTM6 overexpression confers trastuzumab resistance in HER2-positive breast cancer | ||
Exploration (Beijing) Ion cocktail therapy for myocardial infarction by synergistic regulation of both structural and electrical remodeling | ||
Bioact Mater Synergistic mitochondrial homeostasis regulation and cholinergic circuits reconstruction via a one-step synthesized multifunctional hydrogel facilitates spinal cord injury repair. | ||
Nat Commun hnRNPL phase separation activates PIK3CB transcription and promotes glycolysis in ovarian cancer | ||
ACS Nano Microenvironment-Responsive Hydrogels Comprising Engineering Zeolitic Imidazolate Framework-8-Anchored Parathyroid Hormone-Related Peptide-1 for Osteoarthritis Therapy | ||
J Adv Res Dietary neoagarotetraose extends lifespan and impedes brain aging in mice via regulation of microbiota-gut-brain axis |
Reviews
What customers say
Both reviewers rated the product 4 out of 5 stars. One user applied it for Western Blot at a 1:1000 dilution and noted "nice band," indicating clean and specific detection. The other used it for Immunofluorescence and gave a positive rating without additional comments. Overall, customers report satisfactory performance across different applications with good signal quality.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Shriya (Verified Customer) (10-26-2025) |
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Macarena Lucia (Verified Customer) (10-17-2022) | nice band
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