Tested Applications
| Positive WB detected in | A549 cells, A431 cells, HeLa cells, Jurkat cells, K-562 cells, MCF-7 cells, PC-12 cells, NIH/3T3 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human stomach cancer tissue, human colon cancer tissue, human cervical cancer tissue, mouse colon tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells, IFN alpha treated HeLa cells |
| Positive FC (Intra) detected in | Ramos cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10253-2-AP targets STAT3 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, RIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat, pig, canine, monkey, zebrafish, sheep, marmoset |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0360 Product name: Recombinant human STAT3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 2-219 aa of BC000627 Sequence: AQWNQLQQLDTRYLEQLHQLYSDSFPMELRQFLAPWIESQDWAYAASKESHATLVFHNLLGEIDQQYSRFLQESNVLYQHNLRRIKQFLQSRYLEKPMEIARIVARCLWEESRLLQTAATAAQQGGQANHPTAAVVTEKQQMLEQHLQDVRKRVQDLEQKMKVVENLQDDFDFNYKTLKSQGDMQDLNGNNQSVTRQKMQQLEQMLTALDQMRRSIVS Predict reactive species |
| Full Name | signal transducer and activator of transcription 3 (acute-phase response factor) |
| Calculated Molecular Weight | 770 aa, 88 kDa |
| Observed Molecular Weight | 88 kDa |
| GenBank Accession Number | BC000627 |
| Gene Symbol | STAT3 |
| Gene ID (NCBI) | 6774 |
| RRID | AB_2302876 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P40763 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Signal transducer and activator of transcription 3 (acute-phase response factor) (STAT3, synonyms: APRF, FLJ20882, MGC16063) is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT3 is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. STAT3 mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of STAT3. This antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human STAT3. STAT3 exists three isoforms and the molecular weight of each isoform respectively is 83 kDa, 87 kDa and 88 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for STAT3 antibody 10253-2-AP | Download protocol |
| IF protocol for STAT3 antibody 10253-2-AP | Download protocol |
| IHC protocol for STAT3 antibody 10253-2-AP | Download protocol |
| IP protocol for STAT3 antibody 10253-2-AP | Download protocol |
| WB protocol for STAT3 antibody 10253-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The STAT3 Polyclonal antibody (Cat# 10253-2-AP) has 856 total citations. These appear in high-impact journals including Nature, Cell, Cancer Cell, Nature Communications, Nature Cell Biology, Molecular Cancer, Cell Metabolism, Nature Aging, Cell Molecular Immunology, Cancer Research, Nature Microbiology, Autophagy, Journal of Experimental & Clinical Cancer Research, Advanced Science, Cell Death & Differentiation, Cancer Letters, International Journal of Biological Sciences, Oncogene, and Phytomedicine. Research fields span oncology, immunology, inflammation, metabolism, neurology, and fibrosis.
| Species | Application | Title |
|---|---|---|
Cancer Cell Targeting the immune privilege of tumor-initiating cells to enhance cancer immunotherapy | ||
Cell Bacterial immunotherapy leveraging IL-10R hysteresis for both phagocytosis evasion and tumor immunity revitalization | ||
Mol Cancer STAT3-mediated upregulation of lncRNA HOXD-AS1 as a ceRNA facilitates liver cancer metastasis by regulating SOX4.
| ||
Cell Metab Adipokine IL-11/IL-11Ra constrains sphingolipid metabolism to limit the thermogenic capacity of beige adipocytes. | ||
Reviews
What customers say
All seven reviews rate this antibody 5 stars. Users consistently report strong, clear bands in Western Blot, with effective performance also in Immunohistochemistry, Immunofluorescence, and Immunoprecipitation. Recommended dilutions range from 1:1000 to 1:5000 depending on the application. The antibody has been validated across diverse cell types, including liver cells, colon cancer cells, TNBC lines, HeLa, MDA-MB-468, and A375. Reviewers describe it as a reliable, high-quality Stat3 antibody and highly recommend it for multiple applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
James (Verified Customer) (04-23-2026) | Very Good
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Iram (Verified Customer) (03-23-2026) | Very good antibody
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Sai Sindhura (Verified Customer) (03-23-2026) | Good antibody
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Iram (Verified Customer) (09-18-2025) | Bands are very clear band.
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Daniele (Verified Customer) (04-29-2022) | It works very well for WB, IF and IP
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Yuan (Verified Customer) (01-23-2020) | Very good Stat3 antibody for western blot. Strong signal at 1:5000 dilution. Highly recommend.
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Daniele (Verified Customer) (11-25-2019) | It works very well both in western blot and in immunoprecipitation.
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