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Alpha Tubulin Monoklonaler Antikörper
Alpha Tubulin Monoklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Wirt / Isotyp
Maus / IgG2b
Getestete Reaktivität
human, Hund, Maus, Ratte und mehr (5)
Anwendung
WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA
Konjugation
Unkonjugiert
CloneNo.
1E4C11
Kat-Nr. : 66031-1-Ig
Synonyme
"Alpha Tubulin Antibodies" Comparison
View side-by-side comparison of Alpha Tubulin antibodies from other vendors to find the one that best suits your research needs.
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | HeLa-Zellen, 4T1-Zellen, HEK-293-Zellen, HepG2-Zellen, Jurkat-Zellen, K-562-Zellen, NIH/3T3-Zellen |
Erfolgreiche IP | HeLa-Zellen |
Erfolgreiche Detektion in IHC | humanes Tonsillitisgewebe, humanes Kolonkarzinomgewebe, humanes Leberkarzinomgewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
Erfolgreiche Detektion in IF/ICC | HeLa-Zellen, HepG2-Zellen, MCF-7-Zellen |
Erfolgreiche Detektion in FC (Intra) | HeLa-Zellen |
Empfohlene Verdünnung
Anwendung | Verdünnung |
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Western Blot (WB) | WB : 1:20000-1:100000 |
Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunhistochemie (IHC) | IHC : 1:200-1:1000 |
Immunfluoreszenz (IF)/ICC | IF/ICC : 1:500-1:2000 |
Durchflusszytometrie (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
WB | See 1177 publications below |
IHC | See 1 publications below |
IF | See 95 publications below |
IP | See 7 publications below |
CoIP | See 1 publications below |
Produktinformation
66031-1-Ig bindet in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA Alpha Tubulin und zeigt Reaktivität mit human, Hund, Maus, Ratten
Getestete Reaktivität | human, Hund, Maus, Ratte |
In Publikationen genannte Reaktivität | human, Affe, Huhn, Kaninchen, Maus, Ratte, Zebrafisch, Ziege |
Wirt / Isotyp | Maus / IgG2b |
Klonalität | Monoklonal |
Typ | Antikörper |
Immunogen | Alpha Tubulin fusion protein Ag18034 |
Vollständiger Name | tubulin, alpha 1b |
Berechnetes Molekulargewicht | 50 kDa |
Beobachtetes Molekulargewicht | 50-55 kDa |
GenBank-Zugangsnummer | BC009314 |
Gene symbol | Alpha Tubulin |
Gene ID (NCBI) | 10376 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Protein-A-Reinigung |
Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
What is the function of alpha tubulin?
Alpha-tubulin belongs to a large superfamily of tubulin proteins. There are a number of different subtypes that have a molecular weight of ~50kDa and are able to bind to beta-tubulin, forming a heterodimer that polymerises to microtubules as part of the cytoskeleton. These maintain cell structure, provide platforms for intracellular transport and are also involved in cell division.
Where is alpha-tubulin expressed?
Alpha tubulin is highly conserved and is present in nearly all eukaryotic cells as one of the building blocks of microtubules. The ubiquitous nature of this protein has led to its common use as a control protein for many tissue types as well as highlighting the structure of the cytoskeleton.
What are the post-translational modifications of alpha tubulin?
The function and properties of microtubules are drastically affected by the post-translational modifications undergone by tubulin, which may occur to the tubulin dimer directly or to the polymerised mictotubule. For example, the first modification to be identified was detyrosination1, as most alpha-tubulins have a tyrosine at their terminus. This process affects microtubules more than dimers and leads to patches of detyronisation along the structure, regulating protein interactions and allowing subcellular compartments to be defined.2,3 Polyglutamylation also occurs on several sites within the carboxy-terminal tails. However, to date, the most-studied alpha tubulin modification is related to acetylation of lysine 40 (K40).
1. Gundersen, G. G., Khawaja, S. & Bulinski, J. C. Postpolymerization detyrosination of alpha-tubulin: a mechanism for subcellular differentiation of microtubules. J. Cell Biol. 105, 251-64 (1987).
2. Galjart, N. Plus-End-Tracking Proteins and Their Interactions at Microtubule Ends. Curr. Biol. 20, R528-R537 (2010).
3. Jiang, K. & Akhmanova, A. Microtubule tip-interacting proteins: a view from both ends. Curr. Opin. Cell Biol. 23, 94-101 (2011).
Protokolle
PRODUKTSPEZIFISCHE PROTOKOLLE | |
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WB protocol for Alpha Tubulin antibody 66031-1-Ig | Protokoll herunterladen |
IHC protocol for Alpha Tubulin antibody 66031-1-Ig | Protokoll herunterladenl |
IF protocol for Alpha Tubulin antibody 66031-1-Ig | Protokoll herunterladen |
IP protocol for Alpha Tubulin antibody 66031-1-Ig | Protokoll herunterladen |
FC protocol for Alpha Tubulin antibody 66031-1-Ig | Download protocol |
STANDARD-PROTOKOLLE | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
---|---|---|
Nat Biotechnol Magnify is a universal molecular anchoring strategy for expansion microscopy | ||
Cell Res Mitochondria-localized cGAS suppresses ferroptosis to promote cancer progression | ||
Nature Activity-based E3 ligase profiling uncovers an E3 ligase with esterification activity. |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Matthieu (Verified Customer) (09-24-2025) | Band is very clear at the expected size
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FH Bruna (Verified Customer) (07-17-2025) | worked very nice
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FH Jianhua (Verified Customer) (05-28-2025) | Works very well for western blot
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FH Paula (Verified Customer) (02-06-2025) | Works well. Not affected by treatments, therefore reliable loading control. (The band below is TSPO)
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FH Hannah (Verified Customer) (12-02-2024) | Fantastic specific signal with a 1h primary inclubtion and Licor secondary antibody and imaging.
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FH Scott (Verified Customer) (10-22-2024) | 20µg of protein was loaded and antibody was incubated overnight at 4oC following a total protein stain. The band appeared at the expected size. Precision plus protein standard ladder #1610373.
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FH Bartosz (Verified Customer) (08-05-2024) | Great detection, clear bands, and no problem with these antibodies, I strongly recommend them!
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FH Alexandra (Verified Customer) (03-07-2024) | Extremely efficient!
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FH Chiara (Verified Customer) (10-02-2023) | This antibody worked quite well in our immunofluorescence experiments
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FH Udesh (Verified Customer) (08-16-2023) | Worked well for WB at 1:2000
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FH Amy (Verified Customer) (08-10-2023) | Clean strong bands for western blot on HEK293T lysates.
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FH Kalin (Verified Customer) (03-12-2022) | I think this antibody works great for western blots.
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FH Silvia (Verified Customer) (02-18-2022) | This antibody worked quite well in our western blotting and it comes at a great price.
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FH Azita (Verified Customer) (06-02-2021) | Immunohistochemistry labelling of (4% PFA) fixed mice spinal cord tissues using Alpha Tubulin Monoclonal Antibody at dilution of 1:50 showed strong labelling.
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FH Scooby (Verified Customer) (10-05-2020) | happy
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FH Lauren (Verified Customer) (10-05-2020) | thanks
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FH Chun (Verified Customer) (12-05-2019) | An excellent antibody
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