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Alpha Tubulin Monoklonaler Antikörper

Alpha Tubulin Monoklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Hund, Maus, Ratte und mehr (5)

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(1317)

CloneNo.

1E4C11

Kat-Nr. : 66031-1-Ig

Synonyme

TUBA1B, Tubulin alpha 1B chain, 1E4C11, Alpha-tubulin ubiquitous, EC:3.6.5.-


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  • Western Blot (WB) analysis of various lysates using Alpha Tubulin Monoclonal antibody (66031-1-Ig)

    WB analysis using 66031-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66031-1-Ig (Alpha Tubulin antibody) at dilution of 1:200000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using Alpha Tubulin Monoclonal antibody (66031-1-Ig)

    WB analysis of HeLa using 66031-1-Ig

    WB result of Alpha Tubulin antibody (66031-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Alpha Tubulin transfected HeLa cells.

  • Immunoprecipitation (IP) experiment of HeLa cells using Alpha Tubulin Monoclonal antibody (66031-1-Ig)

    IP experiment of HeLa using 66031-1-Ig

    IP result of anti-Alpha Tubulin (IP:66031-1-Ig, 5ug; Detection:11224-1-AP 1:1000) with HeLa cells lysate 2800ug.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using Alpha Tubulin Monoclonal antibody (66031-1-Ig)

    IHC staining of human tonsillitis using 66031-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66031-1-Ig (alpha Tubulin antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Alpha Tubulin Monoclonal antibody (66031-1-Ig)

    IHC staining of human colon cancer using 66031-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66031-1-Ig (Alpha Tubulin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Alpha Tubulin Monoclonal antibody (66031-1-Ig)

    IHC staining of human colon cancer using 66031-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66031-1-Ig (Alpha Tubulin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Alpha Tubulin Monoclonal antibody (66031-1-Ig)

    IHC staining of human liver cancer using 66031-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 66031-1-Ig(alpha Tubulin antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using Alpha Tubulin Monoclonal antibody (66031-1-Ig)

    IHC staining of human tonsillitis using 66031-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66031-1-Ig (alpha Tubulin antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Alpha Tubulin Monoclonal antibody (66031-1-Ig)

    IF Staining of HepG2 using 66031-1-Ig

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed HepG2 cells using 66031-1-Ig(alpha Tubulin antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using Alpha Tubulin Monoclonal antibody (66031-1-Ig)

    IF Staining of MCF-7 using 66031-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11 ) at dilution of 1:1000 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), ZO-1 antibody (21773-1-AP, green).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Alpha Tubulin Monoclonal antibody (66031-1-Ig)

    IF Staining of HeLa using 66031-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11 ) at dilution of 1:1000 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), Histone H1.2 antibody (19649-1-AP, green).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using Alpha Tubulin Monoclonal antibody (66031-1-Ig)

    IF Staining of MCF-7 using 66031-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), ZO-1 antibody (21773-1-AP, red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Alpha Tubulin Monoclonal antibody (66031-1-Ig)

    IF Staining of HepG2 using 66031-1-Ig

    Immunofluorescent analysis of HepG2 cells using 66031-1-Ig (alpha Tubulin antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

  • Flow cytometry (FC) experiment of HeLa cells using Alpha Tubulin Monoclonal antibody (66031-1-Ig)

    FC experiment of HeLa using 66031-1-Ig

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Alpha Tubulin (66031-1-Ig, Clone:1E4C11) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

Erfolgreiche Detektion in WBHeLa-Zellen, 4T1-Zellen, HEK-293-Zellen, HepG2-Zellen, Jurkat-Zellen, K-562-Zellen, NIH/3T3-Zellen
Erfolgreiche IPHeLa-Zellen
Erfolgreiche Detektion in IHChumanes Tonsillitisgewebe, humanes Kolonkarzinomgewebe, humanes Leberkarzinomgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF/ICCHeLa-Zellen, HepG2-Zellen, MCF-7-Zellen
Erfolgreiche Detektion in FC (Intra)HeLa-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:20000-1:100000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:200-1:1000
Immunfluoreszenz (IF)/ICCIF/ICC : 1:500-1:2000
Durchflusszytometrie (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

66031-1-Ig bindet in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA Alpha Tubulin und zeigt Reaktivität mit human, Hund, Maus, Ratten

Getestete Reaktivität human, Hund, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Affe, Huhn, Kaninchen, Maus, Ratte, Zebrafisch, Ziege
Wirt / Isotyp Maus / IgG2b
Klonalität Monoklonal
Typ Antikörper
Immunogen Alpha Tubulin fusion protein Ag18034
Vollständiger Name tubulin, alpha 1b
Berechnetes Molekulargewicht 50 kDa
Beobachtetes Molekulargewicht 50-55 kDa
GenBank-ZugangsnummerBC009314
Gene symbol Alpha Tubulin
Gene ID (NCBI) 10376
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

What is the function of alpha tubulin?

Alpha-tubulin belongs to a large superfamily of tubulin proteins. There are a number of different subtypes that have a molecular weight of ~50kDa and are able to bind to beta-tubulin, forming a heterodimer that polymerises to microtubules as part of the cytoskeleton. These maintain cell structure, provide platforms for intracellular transport and are also involved in cell division.

 Where is alpha-tubulin expressed?

Alpha tubulin is highly conserved and is present in nearly all eukaryotic cells as one of the building blocks of microtubules. The ubiquitous nature of this protein has led to its common use as a control protein for many tissue types as well as highlighting the structure of the cytoskeleton. 

What are the post-translational modifications of alpha tubulin?

The function and properties of microtubules are drastically affected by the post-translational modifications undergone by tubulin, which may occur to the tubulin dimer directly or to the polymerised mictotubule. For example, the first modification to be identified was detyrosination1, as most alpha-tubulins have a tyrosine at their terminus. This process affects microtubules more than dimers and leads to patches of detyronisation along the structure, regulating protein interactions and allowing subcellular compartments to be defined.2,3  Polyglutamylation also occurs on several sites within the carboxy-terminal tails. However, to date, the most-studied alpha tubulin modification is related to acetylation of lysine 40 (K40).

 1. Gundersen, G. G., Khawaja, S. & Bulinski, J. C. Postpolymerization detyrosination of alpha-tubulin: a mechanism for subcellular differentiation of microtubules. J. Cell Biol. 105, 251-64 (1987).

2. Galjart, N. Plus-End-Tracking Proteins and Their Interactions at Microtubule Ends. Curr. Biol. 20, R528-R537 (2010).

3. Jiang, K. & Akhmanova, A. Microtubule tip-interacting proteins: a view from both ends. Curr. Opin. Cell Biol. 23, 94-101 (2011).


Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for Alpha Tubulin antibody 66031-1-IgProtokoll herunterladen
IHC protocol for Alpha Tubulin antibody 66031-1-IgProtokoll herunterladenl
IF protocol for Alpha Tubulin antibody 66031-1-IgProtokoll herunterladen
IP protocol for Alpha Tubulin antibody 66031-1-IgProtokoll herunterladen
FC protocol for Alpha Tubulin antibody 66031-1-IgDownload protocol
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
WB

Nature

Structural basis for FGF hormone signalling

Authors - Lingfeng Chen
humanIF

Nat Biotechnol

Magnify is a universal molecular anchoring strategy for expansion microscopy

Authors - Aleksandra Klimas
mouse,humanWB

Cell Res

AMPK targets PDZD8 to trigger carbon source shift from glucose to glutamine

Authors - Mengqi Li
humanWB

Cell Res

Mitochondria-localized cGAS suppresses ferroptosis to promote cancer progression

Authors - Shiqiao Qiu
monkey,humanWB,IF

Nature

ER proteins decipher the tubulin code to regulate organelle distribution

Authors - Pengli Zheng

Nature

Activity-based E3 ligase profiling uncovers an E3 ligase with esterification activity.

Authors - Kuan-Chuan Pao

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Matthieu (Verified Customer) (09-24-2025)

Band is very clear at the expected size

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: human fibroblats
FH

Bruna (Verified Customer) (07-17-2025)

worked very nice

  • Applications: Western Blot, Immunofluorescence
  • Primary Antibody Dilution: tubulin 1:150 for IF and 1:2000 for WB
  • Cell Tissue Type: mice
Alpha Tubulin Antibody Western Blot,Immunofluorescence validation (tubulin 1:150 for IF and 1:2000 for WB dilution) in mice (Cat no:66031-1-Ig)
FH

Jianhua (Verified Customer) (05-28-2025)

Works very well for western blot

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: human cancer cells
FH

Paula (Verified Customer) (02-06-2025)

Works well. Not affected by treatments, therefore reliable loading control. (The band below is TSPO)

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:10000
  • Cell Tissue Type: Human Bone Marrow Mesenchymal Stem Cells
Alpha Tubulin Antibody Western Blot validation (1:10000 dilution) in Human Bone Marrow Mesenchymal Stem Cells (Cat no:66031-1-Ig)
FH

Hannah (Verified Customer) (12-02-2024)

Fantastic specific signal with a 1h primary inclubtion and Licor secondary antibody and imaging.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/30,000
  • Cell Tissue Type: human fibroblasts
Alpha Tubulin Antibody Western Blot validation (1/30,000 dilution) in human fibroblasts (Cat no:66031-1-Ig)
FH

Scott (Verified Customer) (10-22-2024)

20µg of protein was loaded and antibody was incubated overnight at 4oC following a total protein stain. The band appeared at the expected size. Precision plus protein standard ladder #1610373.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: Mouse Brain Tissue Lysate
Alpha Tubulin Antibody Western Blot validation (1:5000 dilution) in Mouse Brain Tissue Lysate (Cat no:66031-1-Ig)
FH

Bartosz (Verified Customer) (08-05-2024)

Great detection, clear bands, and no problem with these antibodies, I strongly recommend them!

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:20000
  • Cell Tissue Type: SH-SY5Y differentiated/human-origin
FH

Alexandra (Verified Customer) (03-07-2024)

Extremely efficient!

  • Applications: Western Blot
  • Primary Antibody Dilution: 1000x
  • Cell Tissue Type: U2OS human osteosarcoma cell line
Alpha Tubulin Antibody Western Blot validation (1000x dilution) in U2OS human osteosarcoma cell line (Cat no:66031-1-Ig)
FH

Chiara (Verified Customer) (10-02-2023)

This antibody worked quite well in our immunofluorescence experiments

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: human primary skin fibroblasts
Alpha Tubulin Antibody Immunofluorescence validation (1:1000 dilution) in human primary skin fibroblasts (Cat no:66031-1-Ig)
FH

Udesh (Verified Customer) (08-16-2023)

Worked well for WB at 1:2000

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: Bone Marrow MSCs
FH

Amy (Verified Customer) (08-10-2023)

Clean strong bands for western blot on HEK293T lysates.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:50,000
  • Cell Tissue Type: HEK293T
FH

Kalin (Verified Customer) (03-12-2022)

I think this antibody works great for western blots.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:10000
  • Cell Tissue Type: Sea urchin lysate
FH

Silvia (Verified Customer) (02-18-2022)

This antibody worked quite well in our western blotting and it comes at a great price.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: HeLa
FH

Azita (Verified Customer) (06-02-2021)

Immunohistochemistry labelling of (4% PFA) fixed mice spinal cord tissues using Alpha Tubulin Monoclonal Antibody at dilution of 1:50 showed strong labelling.

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1:50
  • Cell Tissue Type: mice spinal cord tissues
FH

Scooby (Verified Customer) (10-05-2020)

happy

  • Applications: Immunofluorescence
  • Cell Tissue Type: n/a
FH

Lauren (Verified Customer) (10-05-2020)

thanks

  • Applications: Western Blot
FH

Chun (Verified Customer) (12-05-2019)

An excellent antibody

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:2,000
  • Cell Tissue Type: HeLa cells